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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Medicinal plants and By-product
- نوع مقاله: Journal Article
- کلمات کلیدی: Phytochemical screening,Enzyme inhibition,Anti-diabetic,Glycogen phosphorylase,Vitis elegans
- چکیده:
- چکیده انگلیسی: In this research, we investigated the antidiabetic activity of Vitis elegans rhizome, which is used as traditional treatment for diabetes mellitus. The methanol, chloroform, petroleum ether, and hexane extracts of the plant root were obtained through serial exhaustive extraction and were analyzed by Thin Layer Chromatography (TLC). Glycogen phosphorylase (GP) assay was done to determine the inhibitory effects of respective extracts on GP enzyme. Total phenol content was measured using the Folin-Ciocalteu method and brine shrimp test was done to evaluate the toxicity of the extracts. Evaluation of TLC plates alone and after spraying with different reagents indicated that terpenoid was the major component of the sample followed by alkaloid and phenol. Chloroform extract applied more inhibitory effects on GP enzyme activity with percentages of 39.65 in concentration of 2.5 mg/ml. This suppression effect was higher than glucose, as a standard inhibitory agent in the body. The highest amount of phenol was found in the methanol extract, equal to 49 mg GAE g-1. Petroleum ether, chloroform and methanol extracts were considered as non-toxic solvents with LC50 values of 8.9, 3.5 and 3.7 mg/ml respectively. While hexane with 0.089 mg/ml LC50 value was classified as toxic extract. Based on the results of this study, we concluded that Vitis elegans rhizome, has the potential to be further studied for anti-hyperglycemic properties.
- انتشار مقاله: 10-05-1396
- نویسندگان: Fatemeh Sadeghi,Jamaludin Bin Mohamad,Samad Muhammadnejad,Mahnaz Haddadi,Mohammad Shirkhoda,Sepideh Mehrzad,Tahereh Barati
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: hypertension,L-citrulline,vasorelaxation,NO,Aortic ring
- چکیده: زمینه مطالعه: دستیابی به یک ترکیب موثر ولی با عوارض کمتر، برای پیشگیری و درمان فشار خون بالا در سال های اخیر مورد توجه بوده است.
هدف: هدف از مطالعه حاضر بررسی تأثیر پیش درمانی سیترولین روی انقباض قطعات ایزوله عروقی جدا شده از آئورت موش صحرایی بوده است.
روشکار: شانزده موش صحرایی نر نژاد ویستار (g 350-300) به صورت تصادفی به دو گروه کنترل و آزمون تقسیم شدند. به گروه شاهد یک میلی لیتر آب مقطر تزریق شد، در حالی که حیوانات گروه آزمون مقدار mg/kg 200 سیترولین از راه صفاقی، برای مدت 7 روز دریافت کردند. آئورت های قفسه سینه آنها بلافاصله بیرون آورده شده و در محلول حاوی (KHS)اKerebs-Henseleit قرار داده شدند. آئورت از بافت های اطراف تمیز شد و در حضور OC2 %5+O2 95% به چند حلقه 3 میلیمتری تقسیم شدند. حلقههای آئورت به 6 زیرگروه تقسیم شدند و در حمام بافت حاوی KHS در دمای C˚37 معلق شدند. حلقههای جدا شده توسط فنیل افرین) (Phe) یا mmol 60 پتاسیم کلراید (KCl) منقبض شدند. پس از ایجاد کفه، غلظت تجمعی از استیل کولین (ACh) برای ایجاد شل شدگی به حمام بافت افزوده شد. اثرات سیترولین بر شل شدگی و نقش نیتریک اکساید (NO) با استفاده از L-NAME به عنوان یک ابزار فارماکولوژیک، مورد بررسی قرار گرفت.
نتایج: تزریق موشها با سیترولین به طور معنیداری (05/0>P) باعث کاهش انقباض عضلانی ناشی از Phe شد. سیترولین همچنین، به طور معنیداری (01/0>P) انقباض ناشی از KCl و L-NAME+Phe را کاهش داد. اما، اضافه شدن دوزهای تجمعی از ACh به طور معنیداری (001/ 0>P) باعث کاهش انقباض ناشی از Phe، اما نه ناشی از KCl و L-NAME+Phe، در گروه کنترل و دریافت کننده سیترولین شد.
نتیجه گیری نهایی: نتایج این مطالعه نشان میدهد که سیترولین میتواند سبب کاهش انقباض عروقی از طریق آزاد کردن NO در رگها شود.- چکیده انگلیسی: Background: The achievement of an effective compound for prevention/treatment of hypertension with fewer complications has been of interest in recent years.
OBJECTIVES: This study aimed to examine the effects of L-citrulline pretreatment on the tension of isolated rat aortic tissues precontracted by different vasoconstrictors.
METHODS: Sixteen male Wistar rats (300-350g) were randomly divided into two groups of control and test. The control group was injected 1ml distilled water, while the animal in the test group received 200 mg/Kg L-citrulline (CIT) i.p. for 7days. Rats were euthanized, their thoracic aortas were immediately separated and placed into a petridish containing cold Kerebs-Henseleit solution (KHS). The aorta were cleaned of the surrounding tissues and cut into 4 rings in the presence of 95% O2+5% CO2. The aortic rings divided into 6 subgroups, were suspended into organ bath containing KHS at 37oC. The isolated rings were contracted by 2×10-6 M phenylephrine (Phe) and 60Mm KCl. When the plateau was reached, a cumulative concentration of acetylcholine (Ach) was added into organ bath to induce relaxation. The effects of CIT on relaxation and the role of NO were tested using L-NAME as a pharmacological probe.
RESULTS: The pretreatment of rats by CIT significantly (P<0.05) reduced the plateau contraction induced by Phe. CIT also significantly (P<0.01) decreased the contraction induced by KCl and L-NAME+Phe. However, cumulative addition of ACh significantly (P<0.001) decreased the vasoconstriction induced by Phe but not by KCl and L-NAME+Phe in both control and CIT-treated group.
CONCLUSIONS: It suggests that, CIT can reduce the rat aorta vasoconstriction through releasing NO.- انتشار مقاله: 12-04-1397
- نویسندگان: Seyyed Reza Hashemi,Hossein Ali Arab,Behjat Seifi,Samad Muhammadnejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: immunohistochemistry,Stomach Neoplasm,Xenograft model,AGS,Athymic nude mice
- چکیده:
- چکیده انگلیسی: Objective(s): Gastric cancer is the third leading cause of cancer-related death worldwide. The overall survival rate of patients is poor because gastric cancers are usually diagnosed at the late stages. Therefore, further research is needed and appropriate research tools are required to develop novel therapeutic approaches.
Materials and Methods: Eight female athymic nude mice with a C57BL/6 background were used in this study. AGS cells were inoculated into the flank. The tumor volumes were calculated and growth curves were drawn. When the volume of the tumors reached 1000 mm3, the animals were humanely euthanized with CO2 gas. After harvesting, tumors were analyzed with Hematoxylin and Eosin (H&E) and immunohistochemistry (IHC). A pathologist confirmed tumor entity through H&E staining. Tumors were evaluated for expression of HER-2, P53, Ki-67, CD34, cytokeratin 8 (CK8), vimentin, estrogen receptor (ER), and progesterone receptor (PR) utilizing immunohistochemistry.
Results: The tumor take rate was 62.5%, mean doubling time was 40.984 d, and the latency period was 30.62 days. The H&E staining results showed highly malignant hyperchromatin epithelial cells. IHC assessment showed the mutation status of P53 gene. The expression score of the CK8 protein in the tumor cells was +3. Vimentin protein was not expressed and changes in mesenchymal phenotype were not observed. Ki-67 IHC indicated that the proliferation rate was >43% and angiogenesis was defined as high MVD.
Conclusion: The respective AGS xenograft model provides an opportunity to understand the pattern of tumor growth as well as to evaluate new gastric cancer therapies in in vivo studies.- انتشار مقاله: 28-01-1396
- نویسندگان: Tahereh Barati,Mahnaz Haddadi,Fatemeh Sadeghi,Samad Muhammadnejad,Ahad Muhammadnejad,Ronak Heidarian,Motahareh Arjomandnejad,Saeid Amanpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Personalized Medicine,Glioblastomamultiform,In vitro chemosensitivity and resistance assay,Primary,explant technique,Stereotactic biopsy
- چکیده:
- چکیده انگلیسی: Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs.
Materials and Methods: Single cell suspension was prepared from 1 g weight explants of the established xenograft tumor of GBM. Also, primary culture was carried out on 35 mg weight specimens, as a surrogate for stereotactic biopsies. Then, chemoresponse profile of cells obtained by direct cell disaggregation and primary culture was determined using temozolomide and carmustine by clonogenic assay[AGA1] .
Results: There was no statistically significant difference in the cytotoxicity of temozolomide and carmustine between cells obtained from both methods.
Conclusion: This work supports the feasibility of using stereotactic biopsies of GBM in CSRAs.- انتشار مقاله: 20-09-1393
- نویسندگان: Fariba Sadeghi Fazel,Mahnaz Haddadi,Alireza Khoshnevisan,Samad Muhammadnejad,Ahad Muhammadnejad,Zohreh Mazaheri,Motahareh Arjomandnejad,Reza Shirkoohi,Mohammad-Ali Oghabian,Narjes Sherkat-Khameneh,Saeid Amanpour,Monireh Kazemimanesh
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: sorafenib,Hepatocellular carcinoma,human placenta Mesenchymal stem cell,animal model
- چکیده:
- چکیده انگلیسی: Background: Hepatocellular carcinoma (HCC) is the fifth most diagnosed cancer and the third leading cause
of cancer-related death. sorafenib is used as a standard therapy to treat HCC. mesenchymal stromal cells (MSCs)
have also been used to suppress HCC. Here we investigate the development of a xenograft model of liver cancer to
study the homing of hpMSC-GFP cells, tumor kinetics and molecular characterizations of HCC. Methods: To create
xenograft models of HCC, HepG2 cell lines were inoculated into the flanks of 9 nude mice bilaterally. Animals were
then divided into three groups: the first group received hpMSC-GFP systemically, the second received intra-tumoral
hpMSC-GFP and the third received PBS. The first two groups were sacrificed after 72 hours of MSCs injection but
the third group was followed up for forty days. One tumor from each animal was then transferred to formalin buffer
for H&E staining and immunohistochemistry analysis (KI67 and CD34), and the other tumor was used for ex-vivo
imaging. Blood samples were taken from all subjects before sacrificing them. Results: Histopathological fidelity of
heterotopic HePG2 xenograft models to human HCC tumors was demonstrated. Biochemical evaluation suggested
the health of the animal’s liver and kidneys. Ex-vivo imaging illustrated homing of more hpMSC-GFP cells in tumor
tissues derived from the group receiving intra-tumoral hpMSC-GFP. Conclusion: A standard method was used to
inoculate tumor cells and the intervention was shown to be safe to liver and kidneys. Local injection of MSCs can be
used as cell therapy to fight neoplasms.- انتشار مقاله: 19-09-1396
- نویسندگان: Saieh Hajighasemlou,Saeedreza Pakzad,Jafar Ai,Samad Muhammadnejad,Milad Mirmoghtadaei,Faezeh Hosseinzadeh,Safoora Gharibzadeh,Amir Kamali,Akbar Ahmadi,Javad Verdi
- مشاهده