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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Sheep,Conjoined twins,Diplopagus,Notomelus,Tribrachius
- چکیده:
- چکیده انگلیسی: Sheep conjoined twins have been reported less than cow. An apparently female conjoined twin lambs was examined based on external and internal features. In radiology, two vertebral columns and two pairs of the ribs were seen. Only two heads and two necks were separated (thoraco-omphalopygopagus). There were three forelimbs (tribrachius), one of which grew on dorsal region as a notomelus. Teat buds of the monsters differed in number. Only one lamb had umbilicus, including one umbilical vein, and two umbilical arteries locating besides one urinary bladder. This lamb had also one uterus. Two-separated alimentary tracts were observed in a common abdomen. Common thorax and abdominal cavities were separated by a diaphragm. There were two esophageal hiatuses, and two caval foramina but only one aortic hiatus. Two pairs of lungs and two unequal and connected hearts in a common pericardium were observed. Abnormality of the circulatory system might have caused the death of the twins.
- انتشار مقاله: 28-11-1392
- نویسندگان: Yazdan Mazaheri,Jamal Nourinezhad,Reza Ranjbar,Mahmood Khaksary Mahabady,Ali Reza Ghadiri,Hamid Lombeshkon
- مشاهده
- جایگاه : پژوهشی
- مجله: Sociological of Studies of youth
- نوع مقاله: Journal Article
- کلمات کلیدی: Social Capital,socio-economic status,cultural capital,religiosity,consumption of cultural goods
- چکیده:
- چکیده انگلیسی: One of the most common methods to identify and represent oneself, especially in contemporary era, is cultural consumption. In cultural consumption, people often consume a variety of valuable goods and symbols, not because of their material value but due to their cultural one in order to create desirable images of themselves in the minds of others. In the meantime, the choice of a particular pattern of cultural consumption or cultural practices or preferences requires cultural resources or cultural capital; or in other words, cultural consumption needs cultural resources and cultural capital. In the present study, the statistical population including 15-29 year olds in Yasuj and a sample size of 379 people were selected. Amos software was used to analyze the data. The results suggested that, there is a relationship between independent variables (religiosity, cultural capital, social capital and socio-economic status), and the consumption of cultural goods. The output of the model also indicates that the variable of cultural capital (0.32) has explained the highest amount of variance of cultural consumption. Moreover, the variables of social capital and religiosity explained 18% and -18 %t of the variance of cultural consumption in the workplace.
- انتشار مقاله: 12-10-1399
- نویسندگان: Seyed Ali Hashemianfar,Ali Seyfzadeh,Parvaneh Bayati,MohammadReza Ranjbar Mohammadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Influenza,HSP70,M2e,N-trimethyl chitosan (TMC),Intra nasal delivery
- چکیده: زمینه مطالعه: شیوع آنفلوانزا در سطح جهانی خطری بالقوه برای بهداشت عمومی در دنیا است. واکسن های مخاطی قادرند سیستم ایمنی عمومی و مخاطی را تحریک کرده و موجب تولید آنتی بادی های IgG و IgA درمخاط شوند، و به این ترتیب دو لایه محافظت در برابر عوامل عفونی نظیر ویروس آنفلوانزاایجاد می شود. سطح مخاطات پوشیده از موکوس میباشد که به طور پیوسته طی مکانیسمی به نام پاکسازی مخاطی مژهای ازسطح مخاط پاک شده و لایه موکوس جدید جایگزین میگردد. بدین ترتیب آنتی ژن تجویز شده از طریق داخل بینی فرصت کمی برای نفوذ به مخاط و تحریک سیستم ایمنی را دارد. هدف: در این مطالعه اثر تری متیل کایتوزان به عنوان حامل موثر برای DNA کدکننده c70e/HSP2M در تجویز داخل بینی در موش مورد بررسی قرار گرفت. روش کار: ژن e2M حراست شده ویروس آنفلوانزا که قادر به ایجاد ایمنی متقاطع در بین گونههای مختلف میباشد، در وکتور 3.1 pcDNAبا قسمت 70c-terminal HSP متصل شده و پس از کپسـوله شدن با یکی از مشتقات کایتوزان به نام تری متیل کایتوزان ازراه داخل بینی تجویز شد. پس از کپسوله شدن پلاسمید ویژگیهای فیزیکی ذرات حاصل توسط دستگاه (Malvern Instruments, UK) 3000 ®Zetasizer بررسی شدند. نتایج: ذرات تولیدی دارای اندازه حدود nm90-120 بوده و دارای بار سطحی مثبت بودند. تجویز پلاسمید c70e/HSP2M به صورت کپسوله با TMC موجب تولید آنتی بـادی IgG در گـردش علیـه e2M شـد کـه میـزان آنتـی بـادی تولیدی تفاوتی معنادار نسبت به تجویزc70e/HSP2M بدون TMC و e2pcDNA/M داشت. نتیجه گیری نهایی: در این تحقیق نشان داده شد که کپسوله کردن c70e/HSP2M در TMC موجب تقویت تحریک سیستم ایمنی هومورال بر علیه c70e/HSP2M شده بدون آنکه تأثیر منفی بر خاصیت کمک ایمن c70HSP داشته باشد.
- چکیده انگلیسی: BACKGROUND: Influenza outbreak has become a great lifethreatening
disease in the world. Nasal vaccines can induce
systemic IgG and mucosal IgA antibody responses, which
establish two layers of immune defense against the infectious
pathogens like influenza. Mucosal vaccines must overcome
several limitations, including the mucociliary clearance and
inefficient uptake of soluble antigens. Therefore, nasal vaccines
require potent adjuvants and delivery systems. OBJECTIVES: In
this study we evaluated the effect of N-trimethyl chitosan (TMC)
as a potent vehicle for DNA encoding M2e/HSP70c in order for
intranasal administration in mice. METHODS:Ectodomain of the
conserved influenza matrix protein 2 (M2e), which has been
found to induce heterosubtypic immunity, was fused to
HSP70359-610 or C-terminus of Mycobacterium tuberculosis
HSP70 (HSP70c) in pcDNA3.1 vector (pcDNA/M2e-HSP70c)
and then encapsulated into a derivative of chitosan, N-trimethyl
chitosan (TMC). After encapsulation of the plasmid, physical
properties of the particles were investigated using Zetasizer®
3000 the particles were then administered through the intranasal
delivery in BALB/c mice. RESULTS: It was found that the
particles had a size ranging between 90-120nm and positive
surface charge. The intranasal immunization with M2e-
HSP70c+TMC in BALB/c mice significantly induced higher
M2e specific IgG than those induced in control groups
(pcDNA/M2e-HSP70c without TMC, pcDNA/M2e, bearing
M2e alone, and PBS).CONCLUSIONS: The present study showed
that the encapsulation of M2e/ HSP70c into N-trimethyl
chitosan (TMC) could strongly induce the humoral immune
response against the M2e-HSP70c plasmid without lowering the
adjuvant efficacy of HSP70c.- انتشار مقاله: 09-12-1391
- نویسندگان: Mehran Dabaghian,Seyyed Mahmoud Ebrahimi,Gholamraza Nikbakhat Borojeni,Majid Tebianian,Ali Rezaei Mokaram,Maryam Iman,Alireza Tavangar Ranjbar
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial effect,Infection,MIC,Well Diffusion,Platelet Rich Plasma
- چکیده:
- چکیده انگلیسی: Background:The utility and efficacy of novel materials in tissue regeneration and antimicrobial therapy are contingent upon the employment of either blood derivatives rich in platelets or platelet-poor-plasma (PPP). This effect is largely mediated by the increased or decreased concentration of platelets in the plasma. The current study aimed to analyze and evaluate the impact of platelet-rich (PRP) or PPP on inhibiting the growth of human pathogenic bacteria and compare their effects with those of chloramphenicol and penicillin.
Methods: In the current comparative study, PRP–1 was generated using 1-step blood centrifugation method; whereas, for PRP–2 and PPP the 2-step centrifugation protocol was used. The antimicrobial activity of PRP–1, 2, and PPP were tested on Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Streptococcus agalactiae, Staphylococcus epidermidis, Shigella sp. and Serratia sp.Well diffusion and serial micro-dilution methods were used for this purpose. Chloramphenicol and penicillin susceptibility were tested using the disk diffusion method.
Results: While whole blood (WB) and PPP had no discernible impact on the growth parameters of any of the bacteria tested in the current study,PRP-1 reduced the growth rate of a few selected strains. In addition, while PRP-2 clearly inhibited the growth of Shigella sp., E. coli, S. aureus, S. agalactiae, and S. epidermidis, it had no impact on the growth of K. pneumoniae, P. aeruginosa,andSerratia sp
Conclusion: It can be claimed that there is a strong correlation between the concentration of platelets and the antibacterial activity of PRP.- انتشار مقاله: 24-09-1395
- نویسندگان: Omid Maghsoudi,Reza Ranjbar,Seyyed Hesamoddin Mirjalili,Mahdi Fasihi Ramandi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Escherichia coli,Polymerase Chain Reaction,DNA fingerprinting,Consensus Sequence
- چکیده:
- چکیده انگلیسی: Background: Escherichia coli is a commensal-pathogenic organism, which includes a wide range of strains. Despite several advanced molecular-genomic technologies for detecting and identifying different strains of E. coli, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) technique is a quick, sharp and cost effective fingerprint method. The major purpose of the present study was to determine the distribution of ERICs within E. coli strains isolated from different healthy animal stool specimens including hens, sheep, and cows, as an appropriate and quick molecular-genomic tool.
Methods: The animal stool samples were obtained during 1 year (October 2012 to October 2013), from animal husbandries around Tehran and Alborz provinces, Iran. After screening processes, the E. coli bacteria were isolated and cultured via standard microbiological methods. The DNA molecules of E. coli bacteria were harvested and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) was applied for bacterial molecular genotyping. The ERIC-PCR products were run on 1% gel electrophoresis. The final images regarding gel electrophoresis banding patterns were used for dendrogram generation via the GelClust software.
Results: Of 120 isolated samples, 115 different strains were recognized as E. coli. The fingerprint patterns involved 380 to 3280 bp bands. The predominant bands included 2900 bp, 1200 bp, and 1200 bp in stool samples of hens, sheep, and cows, respectively. The highest frequencies and diversities were seen among E. coli strains isolated from hens and sheep stool samples.
Conclusion: The DNA profiles were clearly detectable via specific fingerprint patterns. The ERIC-PCR seemed to be a good approach for molecular typing of E. coli strains isolated from different animal sources.- انتشار مقاله: 24-01-1395
- نویسندگان: Reza Ranjbar,Afsar Tabatabaee,Payam Behzadi,Rohollah Kheiri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Antibiotic Susceptibility,Salmonella,ESBL,MDR,DDST
- چکیده:
- چکیده انگلیسی: Background: and Objectives: Salmonella infections are endemic in many developing countries with poor sanitary conditions, but emerge sporadically as a serious public health threat in developed countries. Infections with multidrug resistant (MDR) strains of Salmonella have been associated with treatment failures. Salmonella spp. resistant to extended spectrum cephalosporins are increasing in prevalence worldwide. The aim of this study was to determine the antimicrobial susceptibility, multidrug resistance and extended spectrum beta lactamase (ESBL) production among clinical isolates of Salmonella spp. during 2007 in Tehran, Iran. Patients and Methods: In this cross-sectional study, fifty Salmonella spp. were identified by API 20E system and serotyped by the slide agglutination test. Disk diffusion test was performed. Double disk synergy test was used as a screening test for ESBL production, using disks of cefotaxime and ceftazidime with and without clavulanic acid. Results: From 50 Salmonella spp. 12 (24%) were S. enterica serotype paratyphi B, 24 (48%) S. enterica serotype paratyphi C and 14 (28%) were S. enterica serotype Typhi. The most susceptibility and resistance were observed to ceftazidime (98%) and amoxicillin-clavulanic acid (96%), respectively. 28(56%) were resistant to 5 or more antibiotics. ESBL production was detected by double disk synergy test in one isolate (2%). Conclusion: Results showed increase in antibiotic and multidrug resistance pattern of Salmonella spp. comparing to previous studies in Iran and other countries.It seems that this is the first report of Salmonella spp. ESBL producing in Iran.
- انتشار مقاله: 06-07-1393
- نویسندگان: Ali Jazayeri Moghadas,Gholamreza Irajian,Reza Ranjbar
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Salmonella typhimurium,Multilocus sequence typing,Salmonella infections animal
- چکیده:
- چکیده انگلیسی: Background: Salmonella enterica serovar Typhimurium is one of the most important serovars of Salmonella enterica and is associated with human salmonellosis worldwide. Many epidemiological studies have focused on the characteristics of Salmonella Typhimurium in many countries as well as in Asia. This study was conducted to investigate the genetic characteristics of Salmonella Typhimurium using multilocus sequence typing (MLST).Methods: Clinical samples (urine, blood, and stool) were collected from patients, who were admitted to 2 hospitals in Tehran between April and September, 2015. Salmonella Typhimurium strains were identified by conventional standard biochemical and serological testing. The antibiotic susceptibility patterns of the Salmonella Typhimurium isolates against 16 antibiotics was determined using the disk diffusion assay. The clonal relationship between the strains of Salmonella Typhimurium was analyzed using MLST.Results: Among the 68 Salmonella isolates, 31% (n=21) were Salmonella Typhimurium. Of the total 21 Salmonella Typhimurium isolates, 76% (n=16) were multidrug-resistant and showed resistance to 3 or more antibiotic families. The Salmonella Typhimurium isolates were assigned to 2 sequence types: ST19 and ST328. ST19 was more common (86%). Both sequence types were further assigned to 1 eBURST group.Conclusion: This is the first study of its kind in Iran to determine the sequence types of the clinical isolates of Salmonella Typhimurium in Tehran hospitals using MLST. ST19 was detected as the major sequence type of Salmonella Typhimurium.
- انتشار مقاله: 26-02-1395
- نویسندگان: Reza Ranjbar,Parisa Elhaghi,Leili Shokoohizadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Gestational diabetes,Adenosine deaminase,Single nucleotide polymorphism G22A
- چکیده:
- چکیده انگلیسی: Adenosine deaminase (ADA) is an important regulator of insulin action. The single nucleotide polymorphism (SNP) G22A in the ADA gene decreases enzymatic activity of ADA. The aim of this study was to investigate the relationship between the SNP G22A and blood glycemic control, insulin resistance, and obesity of gestational diabetes mellitus (GDM) patients in an Iranian population. SNP G22A was determined in women with GDM (N=70) and healthy pregnant women (control, N=70) using polymerase chain reaction-restriction fragment length polymorphism. Fasting plasma glucose (FPG), hemoglobin A1C (HbA1c), plasma insulin levels and plasma lipids were measured using commercial kits. Homeostasis model of assessment for insulin resistance (HOMA-IR) was calculated. The distribution of genotypes and alleles among GDM patients was similar to that of the control group. FPG and HbA1c were significantly higher in GDM patients with GG genotype compared with GDM patients with GA+AA genotype and non-GDM patients. The frequency of GG genotype was significantly higher in obese GDM patients compared to lean GDM patients. The SNP G22A in the ADA gene was not associated with the risk of GDM in our population. GG genotype was associated with poor glycemic control and obesity in GDM patients.
- انتشار مقاله: 11-12-1393
- نویسندگان: Mohammad Ali Takhshid,Zinab Zahediannejad,Farzaneh Aboualizadeh,Leili Moezzi,Reza Ranjbaran
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Asthma,Basophil,CD203c,ST2L
- چکیده:
- چکیده انگلیسی: Background: Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms.
Objective: To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma.
Methods: Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR.
Results: Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were foundusing ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p<0.05).
Conclusion: It is proposed that determining CD203c expression by real time PCR may be an effective technique for diagnosis of pediatric asthma.- انتشار مقاله: 14-05-1395
- نویسندگان: Masooma Abdullahi,Reza Ranjbaran,Soheaila Alyasin,Zeinab Keshavarz,Amin Ramezani,Abbas Behzad-Behbahani,Sedigheh Sharifzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Copper,selenium,IL-6,zinc,TNF-α,AMI
- چکیده:
- چکیده انگلیسی: Background: Selenium (Se) is part of the glutathione peroxidase enzyme complex (GSH-PX) that plays an important role in antioxidant mechanisms in body, also it has been demonstrated that populations with low Se intake have 2-3 times greater risk of ischemic heart disease.
Objective: To determine the circulating levels of IL- 6, TNF-α, Cu, Zn, and Se in patients with chronic coronary artery disease (CCAD), acute myocardial infarction (AMI), and normal individuals.
Methods: Patients were divided into two groups: 25 subjects with CCAD and 25 patients with AMI. The control group included 50 normal individuals who did not have any history of ischemic heart disease, and were sex and age matched with the patients. Blood samples were collected during the first hours after the onset of chest pain in AMI group. Serum concentration of Se, Cu, and Zn were determined by atomic absorption spectrometry and TNF-α and IL-6 levels were measured using ELISA method.
Results: In both groups of patients there was a significant reduction in serum Se levels (82.36 + 11.31 mg/L in CCAD, 74.08+11.31mg/L in AMI, and 105+32.52mg/L in the control group, P=0.03). TNF-α titers were increased in AMI patients compared with CCAD and control group. Mean TNF-α levels were 37.44 pg/ml in CCAD, 914.32 pg/ml in AMI, and 4.80 pg/ml in the control group (P=0.01). Serum levels of IL-6 in CCAD and AMI patients were 3.28 ±15.55 pg/ml and 472±207.88 pg/ml, respectively, and 1.28 pg/ml in the control group (P=0.001).
Conclusion: These findings confirm previous studies and demonstrate that patients suffering from AMI exhibit lower plasma concentrations of Se and higher concentrations of pro-inflammatory cytokines of TNF-α and IL-6.- انتشار مقاله: 16-05-1395
- نویسندگان: Mehdi Hassanzadeh,Reza Faridhosseini,Marjane Mahini,Farhad Faridhosseini,Alireza Ranjbar
- مشاهده