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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Dentistry, Shiraz University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rabbit,Osteoclast,Bone Loss,Risedronate
- چکیده:
- چکیده انگلیسی: Statement of the Problem: Alveolar bone resorption due to periodontal disease is common and generally irreversible. It impairs mastication and causes esthetic problems for patients.Bisphosphonates are the most commonly used antiresorptive agents for bone diseases.
Purpose: Considering the risk of bisphosphonate-related osteonecrosis of the jaw, this study aimed to assess the effect of 2% risedronate gel on calvarial bone defects in rabbits.
Materials and Method: In this animal study, 8 mm critical size defect were created in the calvaria of 20 New Zealand white rabbits. In group 1 (n=10), 2% risedronate gel was applied into the right side defect while the left side defect remained empty and served as control. In group 2 (n=10), placebo gel was applied into the right side defect, while the left side defect remained empty and served as control. Five rabbits in each group were sacrificed at 1 and the remaining five at 2 months post-operatively and tissue samples were collected for histomorphometric analysis. Histomorphometric assessments included bone fill, degree of inflammation, number of osteoblasts, number of osteoclasts and foreign body reaction at the site. Data were statistically analysed using SPSS version 25 via the Dunn test and Kruskal-Wallis test.
Results: No bone remodeling was noted in any group at 1 month. The risedronate group showed significantly higher bone fill than the other groups after 2 months (p = 0.016). At 2 months, the number of osteoblasts was significantly higher in the risedronate group (p < 0.05). The groups were not significantly different in terms of inflammation score at 1 (p = 0.31) or 2 (p =0.69) months. Foreign body reaction was not observed in any group at any time point. No osteoclast was detected in any group at any time point.
Conclusion: Risedronate gel showed superior efficacy with regard to regeneration of rabbit calvarial bone defects compared to the placebo and control groups.- انتشار مقاله: 18-09-1398
- نویسندگان: Shabnam Aghayan,Ahmad Asghari,Pejman Mortazavi,Shirin Marzoughi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Testis,Ischemia,Reperfusion,Echinacea purpurae
- چکیده: زمینة مطالعه: ایسکمی رپرفیوژن در بیضه یکی از شرایط ناباروری در مردان است که در طی آسیب ناشی از اکسیداسیون اتفاق می افتد. عصاره گیاه اکیناسه دارای اثرات آنتی اکسیدانی و محافظتی می باشد.
هدف: مطالعه حاضر بمنظور بررسی اثرات عصاره اکیناسه اثرات عصاره بر آسیب ناشی از ایسکمی رپرفیوژن در بیضه موش صحرایی می باشد.
روش کار: در این مطالعه 50 سر موش بالغ نژاد ویستار بطور تصادفی به 5 گروه آزمایشی تقسیم شد: گروه اول کنترل، گروه دوم، 2 ساعت اسیکمی و 24 ساعت رپرفیوژن بیضه چپ، گروه سوم، 2 ساعت ایسکمی که 1 ساعت پس از ایجاد ایسکمی به موش ها عصاره اکیناسه (25 میلی گرم/کیلوگرم) بصورت داخل صفاقی تزریق شد و سپس 24 ساعت رپرفیوژن انجام شد. گروه های 4 و 5 مشابه آزمایش سوم بود و موش ها با سطوح 50 و 100 میلی گرم/کیلوگرم عصاره اکیناسه را دریافت کردند. پس از 24 ساعت، بیضه چپ جدا و برای ارزیابی هیستولوژی و مقادیر آنزیم های سوپراکسیددسموتاز، مالون دی آلدهید، گلوتاتیون پراکسیداز مورد استفاده قرار گرفت.
نتایج: با توجه به نتایج بدست آمده، سطوح مالون دی آلدهید بطورمعنی داری در موش های دچار ایسکمی ریپرفیوژن افزایش پیدا کرد (05/0>P) درحالی که عصاره اکیناسه بطور وابسته به دوز موجب کاهش مالون دی آلدهید شد (05/0>P) . ایسکمی رپرفیوژن تجربی موجب کاهش فعالیت سوپرااکسیددسموتاز و گلوتاتیون پراکسیداز در مقایسه با گروه کنترل شد (05/0>P) . تزریق عصاره اکیناسه (25، 50 و 100 میلی-گرم/کیلوگرم) بطور وابسته به دوز و معنی داری موجب افزایش فعالیت سوپرااکسیددسموتاز و گلوتاتیون پراکسیداز شد (05/0>P) . تجویز عصاره اکیناسه تاثیر معنی داری بر مقادیر توتال آنتی اکسیدان در مقایسه با گروه کنترل نداشت (05/0>P) . در موش های دچار ایسکمی رپرفیوژن لوله های اسپرم ساز تخریب شده و اسپرماتوسیت کمی دیده شد. عصاره اکیناسه (50 و 100 میلی گرم/کیلوگرم) موجب بهبود شاخص های بیضه به همراه توبول های سیمنی فروس و اسپرماتوسیت در مقایسه با گروه ایسکمی رپرفیوژن شد.- چکیده انگلیسی: BACKGROUND: Ischemia/reperfusion of tetsis is a male infertility condition which occurs because of oxidation demage. Echinacea purpurea extract (EPE) has antioxidant and protective effect.
OBJECTIVES: So, the main purpose of this research was to determine effects of EP extract on testicular ischemia/reperfusion (I/R) injury in rat.
METHODS: 50 adult Wistar rats were randomly allocated into five groups: group one as control, group two, 2 hour I/24 hours R period, group three, 1 hour I which after 1 hour of ischemia, rat was injected 25 mg/kg EPE and ischemia continued for an hour, then was followed by 24 hours R period. Groups 4 and 5 were similar to experiment 3, except rats were injected with 50 and 100 mg/kg of EPE, respectively. Then 24 hours later, the left testis was removed for histological and antioxidant enzyme activity including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant status (TAS).
RESUTLS: Based on the findings, MDA concentration was significantly elevated in I/R rat (P<0.05) while EPE diminished MDA concentration in I/R rat (P<0.05). SOD and GPx activity decreased in I/R rat (P<0.05). Injection of the of the EPE (25, 50 and 100mg/kg) increased SOD and GPx concentrations (P<0.05). There was significant fluctuation on TAS in EPE treated groups in comparision to the control group (P>0.05). Seminiferous tubules degenerated and few spermatocytes were observed in testis tubules of the I/R rat. EPE (50 and 100mg/kg) improved testis characteristics in experimental I/R-induced rat in which normal spermatocyte in seminiferous tubules was observed.
CONCLUSIONS: These results suggested EPE has protective effect against against testicular I/R.- انتشار مقاله: 14-11-1397
- نویسندگان: Sadegh Motamedi,Ahmad Asghari,Alireza Jahandideh,Gholamreza Abedi,Pejman Mortazavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Rabbit,Stem cells,defect,PVA,cartilage
- چکیده:
- چکیده انگلیسی: Objective- To evaluate the biological compatibility of differentiated stem cells embedded in poly-vinyl-alcohol (PVA) scaffolds for repair of distal femoral cartilage defect. Design- Experimental in vivo study. Animals- Twelve adult male New Zealand white rabbits were used which were divided into two groups (I, II) six rabbits each. Procedures- Mesenchymal stem cells were isolated from humerus bone marrow of group I rabbits and were cultured and differentiated on PVA scaffolds to chondrocytes. Scanning Electron Microscopy (SEM) showed well distribution of the cells inside the scaffold. A 4 mm diameter full thickness cartilage defect was created on central region of bilateral distal femoral joint surface (patellar groove) in all rabbits. In group (I) the defects were covered with autologous differentiated MSCs-seeded scaffolds; whereas the group II rabbits were left without any treatment as control ones. One month and three months after operation, three rabbits were sacrificed from each group, randomly. Histopathologic evaluation of defects was performed with H&E and trichrome staining. Results- The findings showed that in the engineered cartilage with the PVA scaffold, the defects were filled with smooth, shiny white tissue macroscopically at three months after the transplantation. Despite much connective tissue formed in defect area after three months, there was no evidence of chondrocytes in control group, whereas the defects of experimental group were almost completely filled with hyaline cartilage. Conclusion and Clinical Relevance- The results indicated there is positive possibility for partial resurfacing of cartilage defect using stem cell-seeded PVA scaffolds
- انتشار مقاله: 23-05-1392
- نویسندگان: Davood Sharifi,Pejman Mortazavi,Mohammad Mehdi Dehghan,Parviz Tajik,Mohammad Abedi,Masoud Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Objective- To determine the effect of the transcutaneous electrical neural stimulation on healing of hip joint cartilage defect in rabbit. Design- Experimental in vivo study. Animals- 12 adult New Zealand rabbits were used. Procedures- Under effective the right femoral head was subluxated and the maximum accessible cartilage was denuded up to subchondral bone using dental bit in each rabbit. Then rabbits were divided into two groups of control( I ) with no treatment and treatment group( II) which were subjected to Transcutaneous Electrical Neural Stimulation on 3rd day with frequency of 100 Hz and 80 ?s intensity daily for 10 minutes in 14 days, having 6 rabbits each. These groups further were subdivided into 2 subgroups of 3 rabbits each with duration of one, and three months. The samples were collected for histomorphological study on day 30 and 90 days which were stained with H&E stain. Results- The samples did not show any local reaction on denuded surface in control group; whereas, the fibrous tissue in the central defect, with progression of the healing tissue to fibrocartilage as healing progressed up to 3 months in treated ones. Preliminary results indicate an increased stimulation of the repair tissue, as evidenced by total healing of three of the experimental defects with fibrocartilage as compared to the control specimens. Conclusion and Clinical Relevance- TENS as a physical method of therapy is quite effective in cartilage healing and induces faster remodeling of fibrocartilage fibers.
- انتشار مقاله: 14-05-1386
- نویسندگان: Hussein Jodeiri,Davood Sharifi,Gholamreza Abedi,Iraj Sohrabi Haghdoost,Pejman Mortazavi,Mohammad Abedi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Fisheries Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Hematological parameters,Carbamazepine,Cyprinus carpio
- چکیده:
- چکیده انگلیسی: Tests on pysicochemical parameters in the recent years have confirmed the existence of drug residues and their metabolites in different parts of aquatic environments the permanent release of which has led to micro-persistent pollution. The drugs are designed in a way to be chemically stable, resistant to degradation and to survive by using their biological effects on the organisms. In the present research study, toxicity and the effect of carbamazepine on Cyprinus carpio was examined using blood responses. Thus, the effects of three different concentrations of carbamazepine (1.25, 2.5 and 5 mg L-1) on the changes of blood factors such as red blood cell (RBC), hemoglobin (Hb), hematocrit (Hct), mean cellular volume (MCV), mean cellular hemoglobin (MCH), mean cellular hemoglobin concentration (MCHC), white blood cell (WBC), lymphocyte (Lymph), neutrophil (Neut) and monocyte (Mono) were studied on blood samples collected on days 7, 14 and 21 after exposure to the drug. The results showed that RBC, WBC and lymph counts were reduced in fishes treated with carbamazepine (CBZ). In contrast, the value of Hb, Hct, MCV, MCH, Neut and Mono were increased after exposure to carbamazepine. No significant difference was observed in the MCHC levels in all concentrations. Changes in hematological parameters can act as a biomarker in testing the toxicity of CBZ in aquatic environments. However, detailed studies regarding the application of special biomarkers for assessing human drugs is required.
- انتشار مقاله: 17-12-1395
- نویسندگان: Minoo Rezaei,Ali Mashinchian Moradi,Pejman Mortazavi,Shahla Jamili
- مشاهده