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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Turkey,Avian Influenza,Real Time PCR,Infectious bursal disease virus
- چکیده:
- چکیده انگلیسی: Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected with 104 CID50 of IBDV via intra-bursal route on day 1; Groups 1 and 3 were each infected with 106 EID50of AIV (H9N2) via the oculo-nasal routes on day 30. All groups were vaccinated against Newcastle disease vaccine (NDV). Detection of avian influenza virus H9N2 in trachea and cloaca swabs and in the tissues, was confirmed by Real-time polymerase chain reaction. Anti- NDV–AIV and anti-IBD titers were measured using HI and ELISA tests, respectively. The present study showed that infectious bursal disease changed the pathogenesis of (AIV) H9N2 by affecting AI virus replication and resulted in an increase shedding due to prolonged duration of sever clinical signs. The extent of shedding and virus replication need further study.
- انتشار مقاله: 09-09-1396
- نویسندگان: Farhad Hashemzade,Mansour Mayahi,Abdolhamid Shoshtary,Masoud Reza Seyfi- Abadshapuri,Masoud Ghorbanpoor
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Infectious bronchitis virus,QX-like viruses,S1 gene
- چکیده:
- چکیده انگلیسی: Infectious bronchitis (IB) is a highly contagious disease involving mostly upper respiratory tract in chickens, leading to significant economic losses in the poultry industry worldwide. One of the major concerns regarding to IB is the emergence of new types of infectious bronchitis viruses (IBVs). The purpose of this study was to identify the IBVs isolated from Iranian broiler chickens with respiratory symptoms. Twenty-five broiler flocks around Ahwaz (southwest of Iran) were examined for IBV. The specimens including trachea, lung, liver, kidney, and ceacal tonsil, were collected from diseased birds and inoculated into chicken embryonated eggs. Harvested allantoic fluids were subjected to reverse transcription polymerase chain reaction (RT-PCR) using primers in order to amplify spike 1 (S1) gene of IBV. The RT-PCR products of four IBV isolates were sequenced. The results showed that from 25 examined flocks with respiratory disease, 12 flocks (48.00%) were positive for IBV. In phylogenetic analysis, our isolates were closely related to the QX-like viruses such as PCRLab/06/2012 (Iran), QX, HC9, HC10, CK/CH/GX/NN11-1, CK/CH/JS/YC11-1, CK/CH/JS/2010/13, CK/CH/JS/2011/2 (China), QX/SGK-21, QX/SGK-11 (Iraq) with nucleotide homology up to 99.00%. This study indicates the role of IBVs in the respiratory disorders of broiler flocks located in southwest Iran, and also the existence of a variant of IBV, which is distinguishable from the other Iranian variants.
- انتشار مقاله: 24-05-1397
- نویسندگان: Zahra Boroomand,Ramezan Ali Jafari,Mansour Mayahi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Turkey,RT-PCR,Avian metapneumovirus
- چکیده:
- چکیده انگلیسی: Avian metapneumovirus (aMPV) causes diseases like rhinotracheitis in turkeys, swollen head syndrome in chickens and avian rhinotracheitis in other birds. Causing respiratory problems, aMPV adversely affects production and inflicts immense economic losses and mortalities, especially in turkey flocks. In recent years, several serological and molecular studies have been conducted on this virus, especially in poultry in Asia and Iran. The purpose of the present study was detecting and subtyping aMPV by reverse transcriptase polymerase chain reaction (RT-PCR) from non-vaccinated, commercial turkey flocks in Iran for the first time. Sixty three meat–type unvaccinated turkey flocks from several provinces of Iran were sampled in major turkey abattoirs. Samples were tested by RT-PCR for detecting and subtyping aMPV. The results showed that 26 samples from three flocks (4.10%) were positive for viral RNA and all of the viruses were found to be subtype B of aMPV. As a result, vaccination especially against subtype B of aMPV should be considered in turkey flocks in Iran to control aMPV infections.
- انتشار مقاله: 24-03-1396
- نویسندگان: Mansour Mayahi,Hassan Momtaz,Ramezan Ali Jafari,Pejman Zamani
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Broiler chicken,Alkaline phosphatase,Vitamin E,Malonyldialdehyde,Maltase
- چکیده:
- چکیده انگلیسی: The effects of dietary vitamin E levels on mucosal maltase and alkaline phosphatase (ALP) enzyme activities and on the amount of mucosal malonyldialdehyde (MDA) in broiler chickens were studied in the present study. One hundred and eighty of day old male broiler chicks (Ross 308 strain) were randomly assigned into five groups, each with three replicates and 12 chicks in each replicate. Chickens in group A were fed corn-soy- based diet, while those in groups B, C, D and E were fed the same diet with 20, 60, 180, and 540 mg kg-1 vitamin E supplement (d-alpha tocopherol), respectively. Six birds were randomly chosen from each group, and were euthanized on days 10, 21, 32, and 42 of age. One segment of small intestine outset was homo-genized and mucosal ALP and maltase activity were measured. Moreover, mucosal lipid peroxidate amount was measured to reveal the impact of vitamin E on oxidative stress. Maltase activity was increased with the increase of vitamin E up to 60 mg kg-1 of diet while with further levels, it was decreased. Addition of 60 mg kg-1 of vitamin E to the diet significantly increased ALP enzyme activity (p ≤ 0.001). Addition of 540 mg kg-1 of vitamin E supplement to the diet led to the minimum amount of MDA at 32 days of age. It may be concluded that supplementation of broiler's diet with 60 mg kg-1of vitamin E can increase mucosal maltase and ALP enzyme activity.
- انتشار مقاله: 03-08-1391
- نویسندگان: Seyed Hamid Farrokhifar,Ramezan Ali Jafari,Naeem Erfani Majd,Seyed Reza Fatemi Tabatabaee,Mansour Mayahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Immunogenicity,Gene expression,Eimeria necatrix,microneme
- چکیده: زمینه مطالعه: آیمریا نکاتریکس عامل کوکسیدیوز حداکثر تأثیر اقتصادی را بر روی تولید پرندگان دارد. به نظر میرسد پروتئینهای میکرونم نقش مهمی در حرکت انگل و تهاجم به سلولهای میزبان دارد. هدف: جداسازی و خالصسازی اووسیست آیمریا نکاتریکس از استان خوزستان-ایران بود. یک cDNA کد کننده پروتئین میکرونم 5 (EnMIC5) کلون و پس از بیان کردن این پروتئین نوترکیب، خاصیت ایمنیزایی آن توسط وسترن بلوتینگ مورد آزمایش قرار گرفت. روش کار: یک جفت پرایمر بر اساس توالی نوکلئوتیدی موجود برای ژن میکرونم 5 آیمریا نکاتریکس سویه LZ طراحی و به وسیله آن توالی بخشی از cDNA کد کننده این پروتئین به میزان bp 758 با استفاده از روش Semi- Nested RT-PCR تکثیر گردید سپس قطعه بدست آمده به درون وکتور pMAL-c2x حاوی پروتئین باند شونده به مالتوز کلون و در باکتری E . coli سویه TG1 بیان گردید. قطعه تکثیر شده، یک open reading frame 252 اسیدآمینه ای داشت که به میزان قابل توجهی حاوی مناطق حفاظت شده شامل پری کالکرئین پلاسمایی چسبنده و هفت جزء آبدوست بود. نتایج: در SDS-PAGE پروتئین الحاقی با وزن مولکولی در حدود 70 کیلو دالتون پس از القاء توسط IPTG بیان شد. آزمایش وسترن بلات نشان داد که پروتئین نوترکیب با سرم بدست آمده از جوجههای آلوده به آیمریا نکاتریکس واکنش میدهد. نتیجه گیری نهایی: یافتهها نشان داد پروتئین بدست آمده قدرت ایمنیزایی نسبتاً خوبی داشته و میتواند در آینده مورد مطالعه بیشتری قرار گیرد.
- چکیده انگلیسی: Background: Coccidiosis
caused by Eimeria necatrix has the most economic impact on
poultry production. Micronemal proteins in Eimeria necatrix are thought
to be critical ligands determining host cell specificity at the time of
invasion. OBJECTIVES: Isolation and purification of Eimeria necatrix oocysts from Khuzestan province of Iran was performed. A
cDNA encoding microneme 5 (EnMIC5) protein was cloned and expressed as
recombinant protein before the evaluation of its immunogenicity by Western
blotting. METHODS: A primer pair was designed based on the published
nucleotide sequence of Eimeria necatrix LZ strain micronem5 gene. A
Partial cDNA sequence fragment of 758 bp coding for microneme 5 protein
(EnMIC5) was amplified by semi- Nested RT-PCR. PCR products were cloned and
expressed in a Maltose Binding protein (MBP) containing expression vector
(pMAL-c2x) in Escherichia coli. The cDNA which is encoded for 252 amino
acids shows high degree of conservation. It contains the adhesive plasma
pre-kallikrein and seven hydrophilic motifs. RESULTS: The results of SDS-PAGE
revealed that the recombinant protein with a molecular weight of 70 kDa was
over-expressed after induction with IPTG. Western blotting results revealed
that the expressed recombinant protein was reacted with sera of the chicks
infected with Eimeria necatrix. It was suggested that this protein
should have a good immunogenicity and can be used for further studies. CONCLUSIONS: In conclusion, the high degree of sequence homology indicates that this
protein is immunogenic and might be an
interesting vaccine target, and deserves further investigation- انتشار مقاله: 23-12-1394
- نویسندگان: Mansour Mayahi,Abbas - Jolodar,Sharouz - Masaeli,Hosein Hamidinejat,Masoud - Seyfi Abad Shapouri,Naghme - Moori Bakhtiari
- مشاهده
- جایگاه : پژوهشی
- مجله: Asia Pacific Journal of Medical Toxicology
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی:
- انتشار مقاله: 10-01-1395
- نویسندگان: Zohreh Hosseini,Masoud Ghorbanpoor,Mohammad Khosravi,Mansour Mayahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Mesobuthus eupeus,Venom,Chicken,Pathology,Hematology
- چکیده:
- چکیده انگلیسی: This study aimed to evaluate the clinical, histopathological and hematological effects of Mesobuthus eupeus venom on chicken organs. Adult chickens were subcutaneously injected with five doses of M. eupeus venom (0.5, 2, 5, 10 and 20 mg/kg; four chickens per each dose). Symptoms were recorded during the experiment and blood samples were collected for hematological analysis. Moreover, a complete necropsy was performed. After macroscopic examination, tissue samples were obtained from the liver, kidneys, heart, lungs, intestines and brain of the chickens three days after venom administration. In intravenous injection, lethal dose of the venom was determined at 15 mg/kg. The first clinical, pathological and hematological symptoms in envenomated chickens were observed at M. eupeus doses of 2, 5 and 0.5 mg/kg, respectively. Hematological examination revealed a reduction in lymphocyte count following experimental envenomation, which returned to the pre-experiment level in almost all the cases. On the other hand, heterophil count was found to increase during the experimental period. In addition, erythrocyte count and hematocrit level were stable at all the intervals. Pathological examination was indicative of severe pulmonary hemorrhage, pulmonary and cerebral edema, tubular necrosis of the kidneys, hemorrhage in kidneys and heart, hyaline thrombus and congestion of the liver. According to the results of this study, poultry are resistant to the toxic effects of M. eupeus venom.
- انتشار مقاله: 23-10-1394
- نویسندگان: Mohammad Khosravi,Mansour Mayahi,Seyedeh Missagh Jalali,Anahita Rezaie,Ahmad Taghavi Moghadam,Zohreh Hosseini,Seyedeh Kolsum Barzegar,Somayeh Azadmanesh
- مشاهده