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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Medicinal plants and By-product
- نوع مقاله: Journal Article
- کلمات کلیدی: HPLC,Cell viability,MTT test,Parthenolide,mesoporous silica of PTL
- چکیده:
- چکیده انگلیسی: Background: Parthenolide is major sesquiterpene lactones present in Tanacetum parthenium (L.) Sch.Bip. (feverfew). This compound is known as herbal active principals with potential use in pharmaceutical and medicine. In order to solubility improving, analogue of Parthenolide, aminopropyl theoxy silane -mesoporous silica of Parthenolide, was synthesized as well. In this study, it was extracted from fresh flowers of feverfew and was purified and identified by chromatography methods Cell death of breast cancer cell line MDA-MB-231 was assayed 24 hour after administration of normal and nanoparticle Parthenolide by Methylthiazol Tetrazolium test and Annexin-V-Flous kit and scanning electron microscopy. The results revealed that anti-growth effect of Parthenolide is independent of exposure time and induced apoptosis in cancer cells yet this effect on fibroblast cells as normal ones did not recognized which guarantees the use of this medicinal herb to treat cancers without promotion of other not interested side effect.
- انتشار مقاله: 27-11-1397
- نویسندگان: Nesa Jafary,Zohreh Rabiei,Sattar Tahmasebi Enferadi,Reza Behruzi,Mario Scalet
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Gene expression,Helianthus annuus,Sclerotinia sclerotiorum,oxalic acid
- چکیده:
- چکیده انگلیسی: Background: One of the main sunfl ower diseases is the white mold Sclerotinia sclerotiorum. The oxalic acid (OA), which is one of the main pathogenicity factors of this fungus, beside the direct toxicity on the host, has other functions such as the disruption of the cell wall and chelating out the calcium ions.
Objectives: Regarding the importance of this disease, it is important to study the reactions of the plant against OA which is a nonspecifi c toxin of many other necrotrophic fungi.
Materials and Methods: In this study, two susceptible and moderately resistant sunfl ower lines were inoculated with OA and samples at the fi rst leaf stage were collected within the intervals of 2, 6, 12 and 24 hours post inoculation. The expression of five genes related to tricarboxylic acid cycle, including citrate synthase, fumarase, iso-citrate lyase, malate synthase and malate dehydrogenase was studied under OA treatment.
Results: Two hours after the inoculation, no signifi cant change was observed in the expression of the fi ve studied genes in the moderately resistant line. The iso-citrate lyase gene, which is related to glyoxylate cycle (a variation of the tricarboxylic acid cycle), showed no change in the moderately resistant line; however, it showed an increase in the susceptible line. The increase in fumarase gene expression in moderately resistant line was higher than the susceptible line. The result showed the activation of glyoxylate cycle and destruction of fatty acids in the susceptible line.
Conclusions: Activation of glyoxylate cycle indicated induction of senescent symptoms by OA in susceptible line. Increasing in H2O2 leads to oxidative burst and cell death. Cell death has an apparent benefi t for development and growth of necrotrophic pathogens in the plant cells. The study of resistance mechanisms in response to the pathogen can be useful for breeding programs to provide lines with higher resistance to this pathogen.- انتشار مقاله: 26-11-1395
- نویسندگان: Maryam Monazzah,Zohreh Rabiei,Sattar Tahmasebi Enferadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: HPLC,FT-IR,Cell viability,Harmaline,Harmine,MTT test
- چکیده:
- چکیده انگلیسی: Background: ß-carbolines, harmaline and harmine, are the major alkaloids presenting in the seeds of the Peganum harmala L. These alkaloids are known as herbal active principals with potential use in pharmaceutical and medicine. Objectives: To assess the growth inhibitory effect of phyto-alkaloids, harmaline and harmine, on cancer cell lines. Methods: The P. harmala L.’s alkaloids were extracted by acidic/basic extraction method and identified by two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC). Two breast cancer cell lines, MDA_MB_231, Mcf-7, were subjected to different concentration (1–100 μg.ml-1) of the P. harmala extract at different time courses (24h, 48h, and 72 h). Methylthiazol Tetrazolium (MTT) test, the half maximal inhibitory concentration (IC50) and the morphological changes through optical microscopy were evaluated. Results: In both studied cell lines, the P. harmala extract decreased cell viability in longer time exposure in a dose dependent manner. The more concentrated extract led to higher motility of MDA-MB-231 at 24h. Although, Mcf-7 cell line required longer exposure time has to reach the same motility. It was observed that 30 μg.ml-1 is the minimum lethal dose that kills approximately 50% of cells at 24 hours in MDA-MB-231 cell line (IC50). IC50 for Mcf-7 were calculated 40 μg.ml-1 and 25 μg.ml-1 at 48 h and 72 h, respectively. The morphological observation ensured the apoptosis nature of P. harmala on cells as their membrane kept intact and no membrane permeabilization was observed. Conclusions: The results revealed that the P. harmala extracts decreased significantly growth rate and cell survival of cancer cell lines. The extract induced cell death regarding natural cell growth rate. MDA-MB-231 cell line naturally has a higher growth rate than Mcf-7 cell line, so higher growth inhibition of MDA-MB-231 cell line by the P. harmala extract was confirmed.
- انتشار مقاله: 03-03-1392
- نویسندگان: Sahar Seyed Hassan Tehrani,Somayeh Hashemi Sheikh Shabani,Sattar Tahmasebi Enferadi,Zohreh Rabiei
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: SNPs,genetic analysis,FTIR,cpDNA,Olive Oil Traceability,rbcL Sequence
- چکیده:
- چکیده انگلیسی: Background:Confirmation of olive oil authenticity and particularly virgin olive oil has a great importance. Several advanced chemical and genetic analyses have been used to monitor especial components; however each has its limitations especially when detecting hazelnut-adulterated olive oil. Objectives:The objective of this research is to assess the presence of trace amount of hazelnut oil in olive oil (less than 10%) by Single Base Extension (SBE) and Fourier Transform InfraRed Spectroscopy (FTIR). Materials and Methods:The study was based on the analysis of chloroplast DNA sequences using SBE to detect Single Base Polymorphism (SNPs) in highly preserved DNA regions among species to differentiate pure and adulterated olive oil by means of two parallel tools; ABI PRISM automatic sequencer and the SNaPshot lit (APPLERA). Fourier -Transform InfraRed technique has been used for FTIR spectrum comparisons of pure olive oil and hazelnut-adulterated one as well. Results:Total DNA was extracted successfully from pure and hazelnut-adulterated olive oil, and it provided properly acceptable amplification with the primers designed on chloroplast region of both species and admixture oil of them in different ratios; 50: 50, 70: 30, and vice versa. However, for lesser than 10% hazelnut oil in olive oil only SBE analysis provided recognizable results. FTIR spectra of oil samples were assessed at frequency regions of 4000 - 700 cm-1. Eight wave numbers (3007, 1373, 1237, 1120, 1098, 1032, 965, and 722 cm-1) out of eleven differentiating ones were selected as candidate wave-numbers to distinguish pure and adulterated olive oil. Conclusions:SBE technique proved to be an effective strategy to verify olive oil authenticity, especially from hazelnut-adulterated olive oil. However, FTIR technique provided trustable results only when higher than 10% hazelnut oil is present in olive oil.
- انتشار مقاله: 15-04-1392
- نویسندگان: Leila Akbari,Zohreh Rabiei,Sattar Tahmasebi Enferadi,Sakineh Vanaii
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Olive oil,Simple Sequence Repeats (SSRs),DNA fingerprinting,Genetic traceability
- چکیده:
- چکیده انگلیسی: A reliable DNA extraction method for use on extra virgin olive oil based on a commercial kit was defined, and the possibility of using this DNA for fingerprinting the original cultivar was demonstrated. The genetic traceability of single-cultivar virgin olive oil from two cultivars (Carolea and Frantoio) was achieved by identifying the varieties from which they were produced. This involved the analysis of DNA sequences using a panel of seven simple sequence repeats (SSRs) to provide genotype-specific allelic profiles. The amplified SSR fragments and the DNA profiles from the monovarietal oil corresponded to the profiles from the leaves of the same cultivar. The most reliable SSR in providing correct allele sizing in distinguishing either single-cultivar olive oil samples or the different ratios of their blends are DCA3, DCA4, DCA16, DCA17, and GAPU101, while DCA9, GAPU59 produced less concordance against data obtained by the genetic analysis of leaf samples. To have reproducible results, PCR product purification and selection of a set of markers with a highly robust amplification pattern is suggested.
- انتشار مقاله: 31-01-1394
- نویسندگان: Zohreh Rabiei,Sattar Tahmasebi Enferadi,Abbas Saidi,Sonia Patui,Gian Paolo Vannozzi
- مشاهده