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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Cytotoxic,ANTICP,Pseudo typing,VSVG protein
- چکیده:
- چکیده انگلیسی: Objective(s):This study aims at exploring cytotoxic activity of different peptides derived from VSVG protein against MCF-7 and MDA-MB-231 breast cancer cell lines and human embryonic kidney normal cell (HEK 293).
Materials and Methods: The ANTICP web server was used to predict anticancer peptides. The cytotoxic activity of peptides with high score (P26, P7) and low score (P19) was examined by MTT and DNA fragmentation assays.
Results: The results obtained from ANTICP web serverdemonstrated that 4 out of 48 peptides (P26, P7, P10, and P16) had anticancer activity. P26 and P7 peptides of these 4 peptides were detected to have high cytotoxic activity against MCF-7 cells with CC50 values of 98,280 µg/ml and MDA-MB231 cells with CC50 100,550 µg/ml, respectively. In addition, the results showedthat amino acid residues of these 4 peptides were located near fusion domain.
Conclusion: The results confirmed that P26 and P7 peptides might induce membrane damage and initiate apoptosis. The present study suggested that P26 and P7 peptides could be appropriate candidates for further studies as cytotoxic agents and modifications in the residue at positions 70-280 might potentially produce a more efficient VSVG protein in gene therapy.- انتشار مقاله: 06-11-1393
- نویسندگان: Fereshte Ghandehari,Mandana Behbahani,Abbasali Pourazar,Zahra Noormohammadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,ginger,6- gingerol,PCOS,Estradiol valerate
- چکیده:
- چکیده انگلیسی: Background and aims: PCOS (polycystic ovary syndrome) is the most common endocrine and metabolic disorder characterized by amenorrhea, hyper androgens, hirsutism, chronic anovulation and infertility. The aim of the present study was determining the effects of ginger extract, and 6-gingerol on hormonal levels and ovarian follicles in induced PCOS rats, and comparing the ameliorating effects of these two substances for treatment of PCOS.
Methods: In this experimental research, 42 adult female Wistar rats weighting between 160 g-180 g were divided into six groups of 7 animals. PCOS control that received no injection. PCOS received intraperitoneal injections of 100 mg/kg of ginger extract (for 28 days). Statistical analyses with SPSS, one-way ANOVA, T-test and Duncan test were
used to compare groups.
Results: In comparison with PCOS control, the treatment of PCOS rats with ginger extract (100 and 200 mg/kg) and 6-gingerol (200 and 400 µg/kg) led to significant decrease in LH levels. There was a decrease in FSH levels, but the significant one was only in the 6-gingerol treated group (400 µg/kg). In PCOS treated groups with ginger
extract and 6-gingerol, the serum levels of estradiol decreased significantly compared to control and PCOS control groups (P<0.001). Progesterone levels in PCOS groups injected with ginger extract and 6-gingerol showed a significant increase (P<0.05). In PCOS treated groups with ginger extract and 6-gingerol, testosterone levels decreased
significantly (P<0.001, P<0.01, P<0.05).
Conclusion: 6-gingerol and ginger extract may be a useful treatment for improving the PCOS through reduction of estrogen, testosterone, LH and FSH, and improvement of ovulation. In fact, because of anti-inflammatory and antioxidant properties of ginger components, especially 6-gingerol, they can cause to improve PCOS- انتشار مقاله: 01-02-1396
- نویسندگان: Parisa Sadat Pournaderi,Seyed Hossein Hejazi,Parichehreh Yaghmaei,Hamidreza Khodaei,Zahra Noormohammadi
- مشاهده