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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Phylogenetic analysis,Carotenoids,Ultraviolet radiation,DPPH radicals
- چکیده:
- چکیده انگلیسی: Radiation resistant bacteria have adopted a variety of ingenious strategies for survival under the high dose of radiation, for example through their pigments. In the present study, two ultraviolet-C (UVC) radiation tolerant bacteria, named NM1 and NM3 strains, were isolated from the industrial waste and soil that identified by the molecular analysis. Survival assay of irradiated bacteria was performed by plate counting and flow cytometry (by a fluorescent dye, Rhodamin 123). Also, hydrogen peroxide tolerance of the isolated strains was analyzed by turbidimetric microplate technique. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and EC50 values in reducing power were measured to evaluate antioxidant activity and reductive power of their methanol extracted pigments. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the NM1 and NM3 strains belonged to Microbacterium esteraromaticum and Dietzia schimae with 99% identity, respectively. Both of them showed much high resistance to 15 and 20 J/cm2 UVC irradiation (254nm). Visible spectra of their methanolic extracted pigments were considered identical with λmax at 413, 439 and 468nm for Microbacterium NM1 and λmax at 451nm for Dietzia NM3. EC50 values in reducing power were 35.26 and 36.13 µg/ml for pigments of NM1 and NM3 strains, respectively. Whereas scavenging abilities of DPPH radicals were 3.42 and 1.58 mg/ml for pigments of NM1 and NM3 strains, respectively. Based on the results, the pigments of isolated UVC tolerant bacteria displayed strong antioxidant activity. These bacteria may be a good source for antioxidative-related functional foods and the pharmaceutical industry.
- انتشار مقاله: 23-02-1395
- نویسندگان: Sayyede Narjes Zamanian,Zahra Etemadifar
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Response surface methodology,Alkaline proteases,media optimization,Bacillus sp. ZR-5,detergent compatibility
- چکیده:
- چکیده انگلیسی: The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strain
isolated from the soil, and evaluate its application in detergent industry. The proteolytic activity
of the strain was demonstrated using gelatin hydrolysis screening test. Protease production
optimization was carried out using a two-step approach: a conventional method in order to
identify the best carbon and nitrogen sources followed by the application of response surface
methodology (RSM) to optimize the factors, which include temperature, pH and incubation
time. Glucose or fructose (5 g/L), wheat bran (5 g/L), temperatures of 25 and 55°C, pH 10.0 and
an approximate incubation time of 44 h, were determined as the optimal conditions according to
optimization processes. Validation tests were carried out under these conditions and the results
were in good agreement with RSM predicted data. The in-gel activity (zymogram) test showed
two hydrolytic zones with 66.2 and 36.5 kDa molecular weight on the casein containing
polyacrylamide gel. The high compatibility in the presence of detergent powder and washing
performance test suggested that the crude enzyme could be an appropriate choice as a detergent
additive in detergent industries.- انتشار مقاله: 19-12-1393
- نویسندگان: Hossein Mohammadian,Mohammad Rabbani,Ali Hafizi,Hamid MirMohammad Sadeghi,Amin Davary,Fatemeh Moazen,Ziaedin Samsam Shariat,Zahra Etemadifar
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Response surface methodology,Alkaline proteases,media optimization,Bacillus sp. ZR-5,detergent compatibility
- چکیده:
- چکیده انگلیسی: The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strain
isolated from the soil, and evaluate its application in detergent industry. The proteolytic activity
of the strain was demonstrated using gelatin hydrolysis screening test. Protease production
optimization was carried out using a two-step approach: a conventional method in order to
identify the best carbon and nitrogen sources followed by the application of response surface
methodology (RSM) to optimize the factors, which include temperature, pH and incubation
time. Glucose or fructose (5 g/L), wheat bran (5 g/L), temperatures of 25 and 55°C, pH 10.0 and
an approximate incubation time of 44 h, were determined as the optimal conditions according to
optimization processes. Validation tests were carried out under these conditions and the results
were in good agreement with RSM predicted data. The in-gel activity (zymogram) test showed
two hydrolytic zones with 66.2 and 36.5 kDa molecular weight on the casein containing
polyacrylamide gel. The high compatibility in the presence of detergent powder and washing
performance test suggested that the crude enzyme could be an appropriate choice as a detergent
additive in detergent industries.- انتشار مقاله: 19-12-1393
- نویسندگان: Hossein Mohammadian,Mohammad Rabbani,Ali Hafizi,Hamid MirMohammad Sadeghi,Amin Davary,Fatemeh Moazen,Ziaedin Samsam Shariat,Zahra Etemadifar
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Response surface methodology,Alkaline proteases,media optimization,Bacillus sp. ZR-5,detergent compatibility
- چکیده:
- چکیده انگلیسی: The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strain
isolated from the soil, and evaluate its application in detergent industry. The proteolytic activity
of the strain was demonstrated using gelatin hydrolysis screening test. Protease production
optimization was carried out using a two-step approach: a conventional method in order to
identify the best carbon and nitrogen sources followed by the application of response surface
methodology (RSM) to optimize the factors, which include temperature, pH and incubation
time. Glucose or fructose (5 g/L), wheat bran (5 g/L), temperatures of 25 and 55°C, pH 10.0 and
an approximate incubation time of 44 h, were determined as the optimal conditions according to
optimization processes. Validation tests were carried out under these conditions and the results
were in good agreement with RSM predicted data. The in-gel activity (zymogram) test showed
two hydrolytic zones with 66.2 and 36.5 kDa molecular weight on the casein containing
polyacrylamide gel. The high compatibility in the presence of detergent powder and washing
performance test suggested that the crude enzyme could be an appropriate choice as a detergent
additive in detergent industries.- انتشار مقاله: 19-12-1393
- نویسندگان: Hossein Mohammadian,Mohammad Rabbani,Ali Hafizi,Hamid MirMohammad Sadeghi,Amin Davary,Fatemeh Moazen,Ziaedin Samsam Shariat,Zahra Etemadifar
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Response surface methodology,Alkaline proteases,media optimization,Bacillus sp. ZR-5,detergent compatibility
- چکیده:
- چکیده انگلیسی: The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strain
isolated from the soil, and evaluate its application in detergent industry. The proteolytic activity
of the strain was demonstrated using gelatin hydrolysis screening test. Protease production
optimization was carried out using a two-step approach: a conventional method in order to
identify the best carbon and nitrogen sources followed by the application of response surface
methodology (RSM) to optimize the factors, which include temperature, pH and incubation
time. Glucose or fructose (5 g/L), wheat bran (5 g/L), temperatures of 25 and 55°C, pH 10.0 and
an approximate incubation time of 44 h, were determined as the optimal conditions according to
optimization processes. Validation tests were carried out under these conditions and the results
were in good agreement with RSM predicted data. The in-gel activity (zymogram) test showed
two hydrolytic zones with 66.2 and 36.5 kDa molecular weight on the casein containing
polyacrylamide gel. The high compatibility in the presence of detergent powder and washing
performance test suggested that the crude enzyme could be an appropriate choice as a detergent
additive in detergent industries.- انتشار مقاله: 19-12-1393
- نویسندگان: Hossein Mohammadian,Mohammad Rabbani,Ali Hafizi,Hamid MirMohammad Sadeghi,Amin Davary,Fatemeh Moazen,Ziaedin Samsam Shariat,Zahra Etemadifar
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Alkaline protease,Exiguobacterium,One-factor-at-a-time strategy,Response surface methodology
- چکیده:
- چکیده انگلیسی: Improvement of alkaline protease production, one of the most important types of industrial biocatalysts, is essential for commercial purposes. The present study’s aim was to increase alkaline protease production of MSB42, a newly isolated strain of Exiguobacterium sp., through the rotatable central composite design (RCCD). In this regard, parameters affecting MSB42 enzyme production were chosen based on the results of preliminary one-factor-at-a-time (OFAT) experiments. According to the results of RCCD, the enzyme production showed its maximum level (198.48 U/ml) in the presence of 3% w/v glucose, 3% w/v peptone, at 37 °C and pH 9. Overall, the enzyme production was 2 and 3.24 times more than OFAT experiments (98.69 U/ml) and unoptimized conditions (61.29 U/ml), respectively. A polynomial model of MSB42 alkaline protease production was obtained using the experimental date and based on the significant terms. The protease gene of this strain was also amplified using the designed primers (873 bp), and based on the sequencing results, was found to be closely related to the M6 family of metalloprotease domain.
- انتشار مقاله: 08-03-1398
- نویسندگان: Matia Sadat Borhani,Zahra Etemadifar,Davood Biria,Eisa Jorjani
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Biodesulfurization,4S Pathway,dsz genes,Genetic Engineering,Recombinant Strains
- چکیده:
- چکیده انگلیسی: Biodesulfurization could be a beneficial method in the industry for lowering the sulfur content of crude oil and could improve the efficiency of currently and costly used method, i.e., hydrodesulfurization. But, to achieve this goal, it is critical to enhancing the BDS rate using genetics and other omics approaches. The reason attributed to the fact that the desulfurization activity of currently available natural biocatalysts does not meet the needs of the petroleum industry. Based on the expression of the 4S pathway genes in several heterologous hosts with no ability of desulfurization, the involved genes are ideal targets for enhancement through genetics or metabolic engineering approaches. This review provides an overview of the reported solutions concerning identification of new desulfurizing genes and their regulation, elimination of the inhibitory effects of end products, solving the problem of mass transfer, increasing the production, specific activity, and stability of the involved enzymes, and enhancing the resistance of recombinant strains to the biodesulfurization conditions. Also, the results of some bioinformatics studies which can help in the genetic engineering of desulfurizing strains were provided. Overall, genetic engineering and bioinformatics techniques can be effective in solving the problems of the biodesulfurization process in the near future as a complementary method of hydrodesulfurization.
- انتشار مقاله: 09-06-1398
- نویسندگان: Matia Sadat Borhani,Zahra Etemadifar
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Alkaline protease,Exiguobacterium,One-factor-at-a-time strategy,Response surface methodology
- چکیده:
- چکیده انگلیسی: Improvement of alkaline protease production, one of the most important types of industrial biocatalysts, is essential for commercial purposes. The present study’s aim was to increase alkaline protease production of MSB42, a newly isolated strain of Exiguobacterium sp., through the rotatable central composite design (RCCD). In this regard, parameters affecting MSB42 enzyme production were chosen based on the results of preliminary one-factor-at-a-time (OFAT) experiments. According to the results of RCCD, the enzyme production showed its maximum level (198.48 U/ml) in the presence of 3% w/v glucose, 3% w/v peptone, at 37 °C and pH 9. Overall, the enzyme production was 2 and 3.24 times more than OFAT experiments (98.69 U/ml) and unoptimized conditions (61.29 U/ml), respectively. A polynomial model of MSB42 alkaline protease production was obtained using the experimental date and based on the significant terms. The protease gene of this strain was also amplified using the designed primers (873 bp), and based on the sequencing results, was found to be closely related to the M6 family of metalloprotease domain.
- انتشار مقاله: 08-03-1398
- نویسندگان: Matia Sadat Borhani,Zahra Etemadifar,Davood Biria,Eisa Jorjani
- مشاهده