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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Cell cycle,TMPRSS4,cell apoptosis,Telomere
- چکیده:
- چکیده انگلیسی: Background: TMPRSS4 is a novel Type II transmembrane serine protease found at the surface of the cells and
is involved in the development and cancer progression. However, TMPRSS4 functions in breast cancer remain poor
understand. The present study investigated the function of TMPRSS4 in the breast cancer cells and the potential
mechanistic action underling. Materials and Methods: The lentiviral vectors causing TMPRSS4 down-regulation and
over-expression were established and transfected in MDA-MB-468 and MCF-7 cells, respectively. By using the CCK-
8 assay, cell proliferation was analyzed. Moreover, western blot was used to detect the expression of certain proteins
related to cell apoptosis (Bax and Bcl2) signaling pathway and telomere maintenance (POT1, TPP1, and UBE2D3).
Cell cycle and cell apoptosis were also analyzed by using the Flow cytometry analysis. TMPRSS4 expression was
detected at the mRNA level and protein level by performing qPCR and western blot technique, respectively. Results:
TMPRSS4 expression is inhibited in stable transfected MDA-MB-468-shTMPRSS4 cells compared to the control
MDA-MB-468-NC and its expression is up-regulated in stable transfected MCF-7-TMPTSS4 compared to its control
MCF-7-NC. Moreover, TMPRSS4 silencing in breast cancer reduces cells proliferation by promoting cell cycle arrest
in G2/M phase, cell apoptosis, and telomere maintenance impairment while the TMPRSS4 overexpression increases
cells proliferation through cell apoptosis reduction and telomere maintenance reinforcement associated with insignificant
change in cell cycle progression. Conclusion: TMPRSS4 plays important roles in cancer progression and may be
considered as a good therapeutic target for cancer gene therapy especially breast cancer.- انتشار مقاله: 13-11-1397
- نویسندگان: Ganiou Assani,Akadiri Yessoufou,Yudi Xiong,Julien Segbo,Xiaoyuan Yu,Fuxiang Zhou,Yunfeng Zhou
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Protein kinase CK2,solid tumors,Hematological tumors,CX-4945,CIBG-300
- چکیده:
- چکیده انگلیسی: The Protein kinase CK2 (formerly known as casein kinase 2) is a highly conserved serine/ threonine kinase
overexpressed in various human carcinomas and its high expression often correlates with poor prognosis. CK2 protein
is localized in the nucleus of many tumor cells and correlates with clinical features in many cases. Increased expression
of CK2 in mice results in the development of various types of carcinomas (both solids and blood related tumors, such
as (breast carcinoma, lymphoma, etc), which reveals its carcinogenic properties. CK2 plays essential roles in many key
biological processes related to carcinoma, including cell apoptosis, DNA damage responses and cell cycle regulation.
CK2 has become a potential anti-carcinoma target. Various CK2 inhibitors have been developed with anti-neoplastic
properties against a variety of carcinomas. Some CK2 inhibitors have showed good results in in vitro and pre-clinical
models, and have even entered in clinical trials. This article will review effects of CK2 and its inhibitors on common
carcinomas in in vitro and pre-clinical studies.- انتشار مقاله: 02-03-1397
- نویسندگان: Haiwei Lian,Min Su,Yijie Zhu,Yun Zhou,Shahid Hussain Soomro,Hui Fu
- مشاهده