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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: pH,Juglone,Aptamer,Aptasensor,Redox
- چکیده:
- چکیده انگلیسی: Electrochemical aptamer-based sensors attract a lot of interest as useful methods because of their low cost, accuracy, sensitivity, and simplicity. An electro-active redox molecule comprises the main part of the electrochemical-based sensors. Ferrocene is one of the most popular redox molecule used in biosensor fabrication. But, instability of ferrocenium ion in strong nucleophilic reagents and chloride containing solutions is one of the main problems of this redox molecule. In this study, Juglone is used as an effective quinone redox molecule for aptasensor designing in different pH ranges and different concentrations of chloride ion. The voltammetric studies showed that the electrochemical response of sensor increased by raising the buffer ionic concentration and the sensor accuracy in 7.0 to 8.0 pH range as well. According to the findings, Juglone could be used as an effective redox molecule in high concentrations of chloride containing solutions in the 7.0 pH.
- انتشار مقاله: 24-07-1390
- نویسندگان: Hossein Hamzeiy,Mehdi Saberian,Davoud Asgaria,Yadollah Omidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Nanostructures,Cellular delivery, L-carnitine,Ninhydrin reaction,PAMAM dendrimers,PEGylated PAMAM
- چکیده:
- چکیده انگلیسی: Dendrimer based nanostructures have been increasingly used for delivery of drugs/genes. These nanosystems, as non-viral gene delivery systems, were shown to have relatively high transfection efficiency despite exerting somewhat cytotoxicity. In this current investigation, poly(amido amine) (PAMAM) dendrimers, generation (G) zero to five, PEGylated PAMAM G3 and a new quaternized PAMAM G4 were synthesized and further characterized using FT-IR and 1H-NMR spectroscopies. The cellular uptakes and toxicity of these nanosystems were investigated using fluorescence microscopy and MTT assay, at which they revealed high internaliza-tion potential with low cytotoxicity in both T47D and MCF-7 cells. To establish a simple detection methodology, a ninhydrin reaction was performed on intact PAMAM full generations as well as PEGylated PAMAM G3 and quaternized PAMAM G4. Impacts of various factors such as reaction time, kinetic, reaction medium, and generation dependency of the reaction of dendrimers with ninhydrin were investigated. The best reaction conditions were determined and a simple and reproducible spectroscopic method was established. Upon these findings, we propose that this ninhydrin reaction based methodology may be considered as an easy approach for quantification of primary amines of nanostructured PAMAM dendrimer and its derivatives.
- انتشار مقاله: 06-08-1388
- نویسندگان: Ismaeil Haririan,Alireza Nomani,Rassoul Dinarvand,Ebrahim Azizi,Mohsen Amini,Tarane Gazori,Jaleh Barar,Yadollah Omidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Colon cancer,Akt,cell signaling,Escherichia coli Nissle1917
- چکیده:
- چکیده انگلیسی: Objective(s): Chemotherapies used to treat colon cancer might often fail due to the emergence of chemoresistance and side effects. Escherichia coli Nissle 1917 (EcN) is a beneficial probiotic, whose molecular mechanisms in the prevention of colon cancer are yet to be fully understood. The present study assessed the anti-cancer effects of EcN treatments in human colorectal cancer, HT-29 cell line, with the analysis of related mechanisms.
Materials and Methods: The co-culture conditioned-media (CM) of EcN with adenocarcinoma HT-29 cells and heat-inactivated bacteria (HIB) were applied for the treatment of the HT-29 cells. To study the inhibition potential of CM and HIB on cancer cells, various cellular/molecular analyses were implemented, including DAPI-staining and DNA ladder assays, flow cytometry and Real-time quantitative PCR (qPCR), as well as Western blotting analyses.
Results: Our results indicated that EcN could elicit apoptotic impacts on the colon cancer HT-29 cells by up-regulating PTEN and Bax and down-regulating AKT1 and Bcl-xL genes.
Conclusion: Based on our findings, EcN is proposed as a useful supplemental probiotic treatment against colon cancer.- انتشار مقاله: 16-06-1398
- نویسندگان: Siamak Alizadeh,Abolghasem Esmaeili,Yadollah Omidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Colon cancer,Akt,cell signaling,Escherichia coli Nissle1917
- چکیده:
- چکیده انگلیسی: Objective(s): Chemotherapies used to treat colon cancer might often fail due to the emergence of chemoresistance and side effects. Escherichia coli Nissle 1917 (EcN) is a beneficial probiotic, whose molecular mechanisms in the prevention of colon cancer are yet to be fully understood. The present study assessed the anti-cancer effects of EcN treatments in human colorectal cancer, HT-29 cell line, with the analysis of related mechanisms.
Materials and Methods: The co-culture conditioned-media (CM) of EcN with adenocarcinoma HT-29 cells and heat-inactivated bacteria (HIB) were applied for the treatment of the HT-29 cells. To study the inhibition potential of CM and HIB on cancer cells, various cellular/molecular analyses were implemented, including DAPI-staining and DNA ladder assays, flow cytometry and Real-time quantitative PCR (qPCR), as well as Western blotting analyses.
Results: Our results indicated that EcN could elicit apoptotic impacts on the colon cancer HT-29 cells by up-regulating PTEN and Bax and down-regulating AKT1 and Bcl-xL genes.
Conclusion: Based on our findings, EcN is proposed as a useful supplemental probiotic treatment against colon cancer.- انتشار مقاله: 16-06-1398
- نویسندگان: Siamak Alizadeh,Abolghasem Esmaeili,Yadollah Omidi
- مشاهده