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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: male mice nutrition, Polyunsaturated fatty acids, Sex ratio, Sperm characteristics
- چکیده:
- چکیده انگلیسی: Several studies have indicated that feeding female animal with fatty acids could result in alteration of offspring sex ratio; however, there is little information available regarding the effect of feeding male animal with fatty acids on sex ratio of offspring. The aim of current study was to investigate the effect of paternal feeding with n-3 and n-6 polyunsaturated fatty acids (PUFAs) on sperm characteristics and sex ratio of offspring in mice. Male mice received 2.50% of palm oil (control), fish oil (n-3 PUFAs) or sunflower oil (n-6 PUFAs) for two months. Sperm kinematics and viability were assessed using computer-assisted sperm analyzer and eosin-Y staining, respectively. Additionally, female mice were randomly introduced to males of three experimental groups to determine reproductive parameters including litter size, conception rate and sex ratio. Fish oil enhanced sperm concentration, motility and viability, whereas sunflower oil decreased sperm concentration. Nevertheless, progressive motility, velocity, linearity and straightness of sperms were not affected by source of fatty acids. Although supplementation with fish oil resulted in male-biased sex ratio, palm and sunflower oils did not impact sex ratio. Besides, source of fatty acids failed to influence conception rate and litter size. In conclusion, the present study provided evidence for the impact of paternal fish oil consumption on sex ratio of offspring for the first time. Moreover, it showed beneficial effect of n-3 PUFAs and adverse effects of n-6 PUFAs on seminal parameters.
- انتشار مقاله: 26-05-1397
- نویسندگان: Beheshteh Abouhamzeh,Reza Youssefi,Vahid Akbarinejad,Ehsan Mirsadeghi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Testosterone,Extracellular matrix,Bovine testicular cells
- چکیده:
- چکیده انگلیسی: Testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. Given that extracellular matrix (ECM) facilitates differentiation of spermatogonial stem cells (SSCs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ECM on SSCs differentiation. In experiment 1, testosterone production was measured in testicular cells cultured for 12 days on ECM or plastic (control). In experiment 2, testosterone production was assessed in testicular cells cultured on ECM or plastic (control) and exposed to different concentrations of hCG. In experiment 3, the gene expression of factors involved in testosterone production was analyzed. Testosterone concentration was lower in ECM than in the control group in experiment 1 (p < 0.05). In experiment 2, testosterone concentration was increased in response to hCG in both groups but cells cultured on ECM were more responsive to hCG than those cultured on plastic (p < 0.05). In the experiment 3, qRT-PCR revealed the inhibitory effect of ECM on the gene expression of steroidogenic acute regulatory protein (StAR) (p < 0.05). Nevertheless, the expression of LH receptor was greater in ECM-exposed than in unexposed cells (p < 0.05). In conclusion, the present study showed that inhibiting the expression of StAR, ECM could lower testosterone production by Leydig cells during in vitro culture. In addition, the results indicated that ECM could augment the responsiveness of Leydig cells to hCG through stimulating the expression of LH receptor.
- انتشار مقاله: 24-11-1395
- نویسندگان: Vahid Akbarinejad,Parviz Tajik,Mansoureh Movahedin,Reza Youssefi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Mice,Androgen Receptor,sex ratio,Flutamide
- چکیده:
- چکیده انگلیسی: Maternal testosterone has been indicated to affect sex ratio of offspring. The present study was conducted to elucidate the role of androgen receptor in this regard by blockade of androgen receptor using flutamide in female mice. Mice were randomly assigned to two experimental groups. Mice in the control (n = 20) and treatment (n = 20) groups received 8 IU equine chorionic gonadotropin (eCG) followed by human chorionic gonadotropin (hCG) injection (8 IU) 47 hr later. In addition, mice in the control and treatment groups received four injections of ethanol-saline vehicle and flutamide solution (2.50 mg), respectively, started from 1 hr before eCG injection until hCG injection at 12-hr intervals. Conception rate was not different between the treatment (18/20: 90.00%) and control (19/20: 95.00%) groups (p > 0.05). Litter size was higher in the treatment (8.22 ± 0.26) than control (7.21 ± 0.28) group (p < 0.05). Male sex ratio was lower in the flutamide-treated mice (67/148: 45.30%) as compared with the untreated ones (80/137: 58.40%; odds ratio = 1.69; p < 0.05). In conclusion, the results showed that androgen receptor blockade could skew sex ratio of offspring toward females implying that the effect of testosterone on sex ratio might be through binding to androgen receptor. In addition, the blockade of androgen receptor using flutamide appeared to enhance litter size.
- انتشار مقاله: 09-03-1395
- نویسندگان: Faramarz Gharagozlou,Reza Youssefi,Mehdi Vojgani,Vahid Akbarinejad,Ghazaleh Rafiee
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: bovine,Differentiation,Spermatogonial stem cells,Fibroblast growth factor receptors
- چکیده:
- چکیده انگلیسی: The receptors 1 and 2 of fibroblast growth factor (FGFR1 and FGFR2, respectively) have been observed in all types of testicular cells. Culture on extracellular matrix (ECM) has been observed to lead to initiation of differentiation in spermatogonial stem cells (SSCs). The present study was carried out to investigate whether FGFR1 and FGFR2 play a role in SSCs differentiation. Following isolation, bovine testicular cells were cultured on ECM-coated or uncoated (control) plates for 12 days. The gene expression of THY1, cKIT, FGFR1 and FGFR2 was evaluated using quantitative real-time polymerase chain reaction (PCR). Results related to the gene expression of markers of with undifferentiated (THY1) and differentiated (cKIT) spermatogonia implicated stimulation of self-renewal and differentiation in cells cultured on ECM-coated and uncoated plates, respectively (p < 0.05). Concomitantly, the expression of FGFR2 increased during culture in the ECM group (p < 0.05), whereas it did not change in the control group (p > 0.05). As a result, the gene expression of FGFR2 was greater in the ECM than control group (p < 0.05). Nevertheless, FGFR1 expression did not change during culture in the control and ECM groups (p > 0.05). In conclusion, the present study revealed the potential role of FGFR2 in differentiation of SSCs during culture on ECM.
- انتشار مقاله: 09-03-1395
- نویسندگان: Vahid Akbarinejad,Parviz Tajik,Mansoureh Movahedin,Reza Youssefi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Dog,n-3 Fatty Acids,Secondary sex ratio
- چکیده:
- چکیده انگلیسی: The present study was conducted to evaluate the effect of fish oil supplementation prior to mating on secondary sex ratio of pups (the proportion of males at birth) in bitches. Sixty five bitches (German Shepherd, n = 35; Husky, n = 30) were enrolled in the study. Bitches (140-150 days post-estrus) were given 2% per dry matter intake palm oil and fish oil in the control (n = 33) and treatment (n = 32) groups, respectively. To induce estrus, bitches were received equine chorionic gonadotropin (eCG) administration (50 IU kg-1) 30 days after nutritional supplementation followed by human chorionic gonadotropin (hCG) administration (500 IU per dog) seven days later. Bitches were introduced to dogs of the same breed after hCG administration. The weight of bitches was increased over time (p < 0.05), but their weight change was not different between two groups (p > 0.05). The mating rate, pregnancy rate and litter size were not influenced by treatment and breed. Secondary sex ratio was higher in the treatment (105/164; 64.00%) than in the control (68/147; 46.30%) group (p < 0.05; adjusted odds ratio = 2.068). Moreover, secondary sex ratio was higher in Husky bitches (88/141; 62.40%) compared to German Shepherd (85/170; 50.00%; p < 0.05; adjusted odds ratio = 1.661). In conclusion, the present study showed that inclusion of fish oil in the diet of bitches prior to mating could increase the proportion of male pups at birth. In addition, it appears that there might be variation among dog breeds with regard to the sex ratio of offspring.
- انتشار مقاله: 09-03-1395
- نویسندگان: Faramarz Gharagozlou,Reza Youssefi,Vahid Akbarinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Cat,Follicular ovarian cyst,cystic ovarian disease
- چکیده: در این گزارش، تشخیص و درمان یک مورد کیست تخمدانی فولیکولی در یک گربه پرشین ماده 5 ساله شرح داده میشود. گربه مورد نظر در ملامسه ناحیه پشت کمر، موقعیت لوردوزیس را به خود گرفته و پاهای عقب را بالا و پایین حرکت میداد.بررسی سونوگرافی ناحیه شکم دو کیست در تخمدان چپ گربه نشان داد. ارزیابی استروژن سرم خون، غلظت بالای 17 بتا_استرادیول (pg/mL 105) را نشان داد. با این حال غلظت پروژسترون نرمال (ng/mL 3/0) بود. بر این اساس، گربه مبتلا به کیست فولیکولار فعال تشخیص داده شد. حیوان مذکور با گنادوتروپین جفتی انسان (hCG) به صورت داخل عضلانی تحت درمان قرار گرفت. 30 روز پس از تجویز hCG، یک تزریق گنادوتروپین جفتی اسب (eCG) (50 واحد بین المللی) به صورت داخل عضلانی انجام شد. جفتگیری طبیعی با یک گربه نر پرشین بارور انجام گرفت. در نتیجه، به نظر میرسد که درمان دارویی کیستهای فولیکولار فعال را میتوان به منظور حفظ باروری در گربه سانان به ک ار گرفت.
- چکیده انگلیسی: In the present report, diagnosis and treatment of a case with follicular ovarian cysts in a 5-year-old Persian queen cat is described. In response to palpation of spines, the queen cat presented herself in lordosis and danced up and down with her rear legs. Trans-abdominal ultrasonography examination showed 2 cysts in the left ovary of the queen. Serum estrogen assay indicated elevated level of 17 β-estradiol concentration (105 pg/ml). However, progesterone concentration was normal (0.3 ng/ml). Accordingly, the queen was diagnosed with functional follicular cysts. The queen was treated with an administration of hCG intra-muscularly. Thirty (30) days after the administration of hCG, an injection of equine chorionic gonadotropin (eCG) (50 IU) was given intra-muscularly. Natural mating was done with a fertile Persian tom cat. In conclusion, it seems that treatment of functional follicular cysts can be applied to preserve fertility in cats.
- انتشار مقاله: 22-10-1393
- نویسندگان: Reza Youssefi,Parviz Tajik,Vrya Tohidi,Vahid Akbarinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: mastitis,Dairy cow,Bulk tank milk
- چکیده: زمینه مطالعه: ورم پستان زیان بار ترین بیماری در صنعت گاو شیری به لحاظ اقتصادی می باشد. ارزیابی شیر تانک جمع آوری شیر به عنوان روشی جهت مانیتور کردن وضعیت سلامت پستان در سطح گله پیشنهاد شده است. هدف: مطالعه ای به روش مقطعی به منظور ارزیابی وضعیت سلامت پستان در 15 گله گاو شیری هلشتاین واقع در استان قم به انجام رسید. روش کار: شمارش سلولهای سوماتیک شیر تانک جمع آوری شیـر بـا استفـاده از دستگـاه فـوزومـاتیـک صـورت پـذیرفت. شمارش پلیت استاندارد، شمارش انکوباسیون اولیه، شمارش پاستوریزاسیون آزمـایشگـاهـی و تعـداد استـرپتـوکـوکـوسهـای محیـطـی، استـرپتوکوکوس آگالاکتیه، استرپتوکوکوس دیس گالاکتیه، استرپتوکوکوس یوبریس،ای کولای، کلبسیلا نومونیه، استافیلوکوکوس های کوآگولاز منفی، استافیلوکوکوس اورئوس و کورینه باکتریوم بوویس با استفاده از محیطهای کشت اختصاصی انجام شد. بعلاوه، تشخیص ورم پستان بالینی از طریق معاینه هر کارتیه و تشخیص ورم پستان تحت بالینی توسط تست ورم پستان کالیفرنیایی صورت پذیرفت. نتایج: غالب گله های مورد بررسی مقادیر متوسط تا بالای شمارش سلولهای سوماتیک شیر تانک جمع آوری شیر و مقادیر بالای شمارش باکتریایی شیر تانک جمع آوری شیر را دارا بودند. شیوع ورم پستان بالینی و تحت بالینی به ترتیب 3/1 % و 7/24% بود. نتیجه گیری نهایی: مطالعه حاضر بیانگر وضعیت نامناسب مدیریت جایگاه، بهداشت پستان و شیردوشی در گلههای مورد بررسی می باشد.
- چکیده انگلیسی: BACKGROUND:Mastitis is the most economically important
disease in dairy industry worldwide. Bulk tank milk (BTM)
analysis has been suggested for monitoring the udder health
status at herd level. OBJECTIVES: A cross-sectional study was
conducted to evaluate udder health status in 15 Holstein dairy
farms located in Qom province. METHODS: Bulk tank somatic
cell count (BTMSCC) was analyzed using opto-fluoroelectronic
counter. Standard plate count, preliminary incubation
count, laboratory pasteurized count, and the number of
environmental streptococci, S. agalactiae, S. dysgalactiae, S.
uberis, E. coli, K. pneumoniae, coagulase negative streptococci,
S. aureus and C. bovis were determined using specific culture
media. Moreover, clinical and subclinical mastitis were diagnosed
using physical examination and California mastitis test,
respectively. RESULTS: Most herds had moderate to high
BTMSCC and high bacterial counts. The prevalence of clinical
and subclinical mastitis was 1.3% and 24.7%, respectively.
CONCLUSIONS: The present study indicated inefficient stall
management, udder hygiene, and milking practices in the herds
investigated.- انتشار مقاله: 26-01-1393
- نویسندگان: Tayebeh Mahmoodi Afsah,Mehdi Vojgani,Faramarz Gharagozlou,Vahid Akbarinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Inflammation,Silymarin,Ischemia,Reperfusion
- چکیده:
- چکیده انگلیسی: Ischemia-reperfusion injuries (IRI) occur in different clinical conditions such as stroke, trauma, organ transplantation, and so on. Ischemia damages mainly arise from oxygen depletion in tissues. The lack of oxygen as the last acceptor of electron in the respiratory chain causes a decrease in ATP production and eventually leads to disruption of membrane transport, acidosis, cellular edema and membrane distortion of organelles, and cells. Reperfusion can intensify ischemic injuries by the infiltration of inflammatory cells and also oxygen and calcium overloading. Since the tissue antioxidant contents decreased due to increased generation of reactive oxygen species (ROS) during IRI, the application of antioxidants is considered an appropriate strategy to ameliorate IRI. Silymarin constitutes about 70–80% of silybum marianum dry extract and is known as a strong free radical scavenger with anti-inflammatory properties. In several studies, silibinin as a major component of Silymarin could provide protective effects in various tissue IRI by different mechanisms such as scavenging free radicals, decreasing inflammatory cytokines, inhibiting cellular death, and increasing the expression of antioxidant enzymes. To clarify functional mechanisms, the present article evaluates studies about silymarin effects in different tissues IRI.
- انتشار مقاله: 27-05-1397
- نویسندگان: Vahid Akbari-korhkheyli,Kazem Abbaszadeh-Goudarzi,Mohaddeseh Nejati-Laskokalayeh,Setareh Zarpou,Abbas Khonakdar-tarsi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Inflammation,Silymarin,Ischemia,Reperfusion
- چکیده:
- چکیده انگلیسی: Ischemia-reperfusion injuries (IRI) occur in different clinical conditions such as stroke, trauma, organ transplantation, and so on. Ischemia damages mainly arise from oxygen depletion in tissues. The lack of oxygen as the last acceptor of electron in the respiratory chain causes a decrease in ATP production and eventually leads to disruption of membrane transport, acidosis, cellular edema and membrane distortion of organelles, and cells. Reperfusion can intensify ischemic injuries by the infiltration of inflammatory cells and also oxygen and calcium overloading. Since the tissue antioxidant contents decreased due to increased generation of reactive oxygen species (ROS) during IRI, the application of antioxidants is considered an appropriate strategy to ameliorate IRI. Silymarin constitutes about 70–80% of silybum marianum dry extract and is known as a strong free radical scavenger with anti-inflammatory properties. In several studies, silibinin as a major component of Silymarin could provide protective effects in various tissue IRI by different mechanisms such as scavenging free radicals, decreasing inflammatory cytokines, inhibiting cellular death, and increasing the expression of antioxidant enzymes. To clarify functional mechanisms, the present article evaluates studies about silymarin effects in different tissues IRI.
- انتشار مقاله: 27-05-1397
- نویسندگان: Vahid Akbari-korhkheyli,Kazem Abbaszadeh-Goudarzi,Mohaddeseh Nejati-Laskokalayeh,Setareh Zarpou,Abbas Khonakdar-tarsi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Arrhythmia,Jervell and Lange-Nielsen syndrome,KCNQ1,Long-QT syndrome,Romano-Ward syndrome
- چکیده:
- چکیده انگلیسی: Objective(s): Jervell and Lange–Nielsen syndrome is an autosomal recessive disorder caused by mutations in KCNQ1 or KCNE1 genes. The disease is characterized by sensorineural hearing loss and long QT syndrome.
Methods: Here we present a 3.5-year-old female patient, an offspring of consanguineous marriage, who had a history of recurrent syncope and congenital sensorineural deafness. The patient and the family members were screened for mutations in KCNQ1 gene by linkage analysis and DNA sequencing.
Results: DNA sequencing showed a c.1532_1534delG (p. A512Pfs*81) mutation in the KCNQ1 gene in homozygous form. The results of short tandem repeat (STR) markers showed that the disease in the family is linked to the KCNQ1 gene. The mutation was confirmed in the parents in heterozygous form.
Conclusion: This is the first report of this variant in KCNQ1 gene in an Iranian family. The data of this study could be used for early diagnosis of the condition in the family and genetic counseling.- انتشار مقاله: 17-02-1396
- نویسندگان: Azam Amirian,Seyed Mohammad Dalili,Zahra Zafari,Siamak Saber,Morteza Karimipour,Vahid Akbari,Amirfarjam Fazelifar,Sirous Zeinali
- مشاهده