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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Mutation,gallbladder cancer,tumor suppressor gene,proto-oncogene,Peru
- چکیده:
- چکیده انگلیسی: Background: Recent studies have shown that genetic alterations are associated with the effect of patient geographic
location on gallbladder cancer development. Peru has a high incidence of gallbladder cancer, but causative factors
have not yet been identified. We examined the frequency of mutations in TP53 and K-ras genes in Peruvian patients
with gallbladder cancer, and compared this with data from Bolivia, Hungary, Chile, and Japan, which have a high
gallbladder cancer incidence. Methods: DNA was extracted from formalin-fixed paraffin-embedded gallbladder tissue
sections of 30 gallbladder cancer patients (9 men and 21 women) obtained using microdissection. Mutations in exons
5 to 8 of TP53 and codons 12, 13, and 61 of K-ras were examined using direct sequencing. Results: TP53 mutations
were observed in 10 (33.3%) of patients, but K-ras mutations were absent. Nine (90%) TP53 mutations were point
mutations (7 missense and 2 silent mutations), and the most frequent substitution was a G:C to A:T transition. G:C to
A:T transitions at the CpG site or G:C to T:A transversions were found in one patient each. No significant differences
were found in the frequency of TP53 and K-ras mutations among patients in the 5 countries. Conclusions: Our findings
suggest that endogenous mechanisms and exogenous carcinogens may affect the carcinogenic process in Peruvian
gallbladder cancer patients, similar to that in Bolivian patients. Further studies with a larger sample size are needed
to clarify these findings.- انتشار مقاله: 02-07-1397
- نویسندگان: Tatiana Vidaurre,Sandro Casavilca,Paola Montenegro,Henry Gomez,Mónica Calderón,Jeannie Navarro,Jessica Aramburu,Ebert Poquioma,Yasuo Tsuchiya,Takao Asai,Yoichi Ajioka,Ayako Sato,Toshikazu Ikoma,Kazutoshi Nakamura
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Helicobacter pylori,gallbladder cancer,autoanalyzer,latex agglutination assay
- چکیده:
- چکیده انگلیسی: Objective: Gallbladder cancer is the commonest gastrointestinal cancer in northern Indian women. Some studies
have examined the association between Helicobacter pylori infection and gallbladder cancer risk, but findings have been
inconsistent. We aimed to examine the association between H. pylori infection and gallbladder cancer in Indian people.
Materials and Methods: We conducted a hospital-based case-control study including 100 gallbladder cancer patients
with gallstones who were 32 to 79 years old (cases; 72 women and 28 men), and 100 cholelithiasis patients aged 14 to
75 years (controls; 65 women and 35 men). All patients had a diagnosis of gallbladder cancer or cholelithiasis at the
Sanjay Gandhi Post Graduate Institute of Medical Sciences in Lucknow having a high gallbladder cancer incidence
in northern India, from May 2014 through July 2017. Plasma samples were collected from all patients before surgical
treatment. Plasma H. pylori antibody titer was measured by the latex agglutination method and an autoanalyzer. H.
pylori infection was defined as antibody titer ≥10 U/mL. Plasma antibody titers and H. pylori infection positivity rates
were compared between cases and controls. Results: Mean plasma antibody titers (standard deviation, range) were
11.1 U/mL (11.6, 0–78) in cases and 13.6 U/mL (23.0, 1–164) in controls. H. pylori infection positivity rates were
41% and 42% in cases and controls, respectively. No significant differences in antibody titers or H. pylori infection
positivity rates were found between cases and controls. Conclusions: We found no evidence of H. pylori infection as
an important risk factor for gallbladder cancer in Indian people.- انتشار مقاله: 24-11-1396
- نویسندگان: Yasuo Tsuchiya,Kumudesh Mishra,Vinay K Kapoor,Ruchira Vishwakarma,Anu Behari,Toshikazu Ikoma,Takao Asai,Kazuo Endoh,Kazutoshi Nakamura
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: gallbladder bile,next generation sequencing,16S rRNA,V3-V4 region,gallbladder cancer
- چکیده:
- چکیده انگلیسی: Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but
findings have been inconsistent. Other bacterial infections may also be associated with GBC. However, information on
microbial pathogens in gallbladder bile of GBC patients is scarce. We aimed to investigate the microbial communities in
gallbladder bile of patients with GBC and cholelithiasis (CL). Seven GBC patients and 30 CL patients were enrolled in
this study. Genomic DNA was extracted from bile and the V3-V4 region of 16S rRNA was amplified. The sequencing
results were compared with the 16S database, and the bacteria were identified by homology searches and phylogenetic
analysis. DNA was detected in the bile of three GBC (42.9%; Bolivia, 1; Chile, 2) and four CL patients (13.3%; Bolivia,
1; Chile, 3). Of the 37 patients, 30 (81.1%) were negative and unable to analyze. Salmonella typhi and Helicobacter sp.
were not detected in bile from any GBC patients. As the predominant species, Fusobacterium nucleatum, Escherichia
coli, and Enetrobacter sp. were detected in bile from GBC patients. Those in bile from CL patients were Escherichia
coli, Salmonella sp., and Enerococcus gallinarum. Escherichia coli was detected in bile samples from both GBC and
CL patients. Whether the bacteria detected in bile from GBC patients would associated with the development of GBC
warrant further investigation.- انتشار مقاله: 29-06-1396
- نویسندگان: Yasuo Tsuchiya,Ernest Loza,Guido Villa-Gomez,Carlos C Trujillo,Sergio Baez,Takao Asai,Toshikazu Ikoma,Kazuo Endoh,Kazutoshi Nakamura
- مشاهده