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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Screening,Colorectal cancer,Colonoscopy,Polyps,Stool DNA
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health. Aim: to analyze stool DNA by human DNA quantify and microarray methods as alternatives to CRC screening. Method: Three methods were analyzed in stool samples: Human DNA Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays. Results: KBP array mutations were presented in 60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity and RanplexCRC Array method had 78% sensitivity and 100% specificity. Conclusion: Microarray methods showed promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy and applicability by clinical routine.
- انتشار مقاله: 23-11-1397
- نویسندگان: Jacqueline Lima,Yolanda Teixeira,Célia Pimenta,Aledson Vitor Felipe,Tiago Donizetti Silva,Ermelindo Ermelindo Della Libera Junior,Sarhan Sydney Saad,Elisabeth Deak,Helena Murray,Nora Manoukian Forones
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Drug resistance,Gastric cancer,RNA interference,ABCB1,epirubicin
- چکیده:
- چکیده انگلیسی: Background: Gastric cancer is one of the most common malignancies worldwide. Epirubicin (EPI) is used
extensively in the treatment of multiple cancers despite its tendency to induce multidrug resistance though overexpression
of the ABCB1 efflux pump. However, this overexpression can be disrupted using short interfering RNAs (siRNAs).
Objective and Methods: The aim of this study was to explore approaches to reverse EPI resistance and thus increase
the success of chemotherapy treatment in an EPI-resistant gastric cancer cell subline (AGS/EPI). Methods: The study
focused on effects of ABCB1 knockdown by siRNA technology using TaqMan gene expression assays with quantitative
real-time reverse-transcription PCR (qRT-PCR). MTT assays were performed to evaluate viability and prolifer in subline.
ABCB1 protein localization and EPI intracellular fluorescence intensity in AGS/EPI cells were detected by confocal
microscopy. Results: The siRNA efficiently downregulated ABCB1 mRNA in AGS/EPI cells. Thus MDR reversal
was clearly demonstrated in the AGS/EPI cells, offering the possibility of future in vitro chemoresistance assays for
the GC field. Conclusions: ABCB1 knockdown decreased EPI efflux and increased EPI sensitivity in AGS/EPI cells.
This result provides a novel strategy for targeted gene therapy to reverse EPI resistance in gastric cancer.- انتشار مقاله: 18-04-1396
- نویسندگان: Aledson Vitor Felipe,Juliana De Oliveira,Andrea Aparecida Moraes,Jerônimo Pereira De França,Tiago Donizetti Da Silva,Nora Manoukian Forones
- مشاهده