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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Pakistan,AML,adult,cytogenetics
- چکیده:
- چکیده انگلیسی: Objectives: The heterogenous response to treatment in acute myeloid leukemia (AML) can be attributed largely to
the difference in cytogenetic features identified in between cases. Cytogenetic analysis in acute leukemia is now
routinely used to assist patient management, particularly in terms of diagnosis, disease monitoring, prognosis and risk
stratification. Knowing about cytogenetic profile at the time of diagnosis is important in order to take critical decisions
in management of these patients. The study was conducted to determine the distribution of cytogenetic abnormalities in
Pakistani adult patients with AML in order to have insights regarding behavior of the disease. Methods: A retrospective
analysis of all the cases of AML (≥15years old) diagnosed at Aga Khan University from January 2011 to December 2016
was performed. Cytogenetic analysis was made for all cases using the trypsin-Giemsa banding technique. Karyotypes
were interpreted using the International System for Human Cytogenetic Nomenclature (ISCN) criteria. Results:
A total of 321 patients were diagnosed with AML during the study period, of which 288 samples successfully yielded
metaphase chromosomes. The male to female ratio was 1.7:1. A normal karyotype was present in 61% (n=176) of
the cases whereas, 39% (n=112) had an abnormal karyotype. Of the abnormal cases, t (8;21) (q22;q22) and t (15;17)
(q22;q12) were identified in 8.3% and 4.9% cases respectively. Adverse prognostic cytogenetic subgroups including
complex karyotype, monosomy 7 and t(6;9)(p23;q34) were identified in 9%, 1% and 0.7% patients respectively.
Conclusions: This largest cytogenetic data in adult AML from Pakistan showed comparable prevalence of favorable
prognostic karyotype to international data. The prevalence of specific adverse prognostic karyotype was low.- انتشار مقاله: 03-11-1396
- نویسندگان: Muhammad Shariq Shaikh,Zeeshan Ansar Ahmed,Mohammad Usman Shaikh,Salman Naseem Adil,Mohammad Khurshid,Tariq Moatter,Anila Rashid,Farheen Karim,Ahmed Raheem,Natasha Ali
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Lung adenocarcinoma,IHC,ALK,fish
- چکیده:
- چکیده انگلیسی: Anaplastic lymphoma kinase (ALK) gene can be oncogenic either by forming fusion with other genes, amplification
of the gene or by having mutations. ALK rearrangement can either be detected by standard “fluorescence in situ
hybridization (FISH)” or “immunohistochemistry (IHC)”. Objective of this study was to record the prevalence of
ALK rearrangement in adenocarcinoma of Primary Lung origin and compare it with ALK-IHC staining. Data of
64 patients of lung adenocarcinoma from 2015-2017 was analyzed. All of the FFPE biopsies were tested for EGFR
(qPCR) followed by ALK rearrangement (by FISH and IHC) on EGFR negative samples. Out of 64 samples, 21.8%
(14) showed EGFR mutations and 14% (7/50) were positive for ALK rearrangement when checked by FISH. In IHC
testing for ALK (FISH positive) 8% (4/50) showed positivity. In conclusion ALK-FISH positive cases are higher than
other studies likely due to the relatively small sample size. FISH testing was found to be more sensitive than IHC; one
reason may be the low level of ALK. Our study warrants that currently FISH remains the gold standard for screening
of ALK gene rearrangements.- انتشار مقاله: 20-09-1396
- نویسندگان: Samar Moattar,Namrah Anwar,Tariq Moatter,Shahid Pervez
- مشاهده