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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: immunohistochemistry,HER2,Insitu Hybridization,Skp2,Colonic Lesions
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is the fourth most common cancer worldwide. Both HER2 and SKP2 have a carcinogenic role in CRC making them attractive targets for tailored treatment. This work aims to correlate HER2 and SKP2 protein expression as well as HER2 gene amplification with clinicopathological parameters aiming at identifying potential candidates for targeted therapy. Methods: This Study was conducted on 127 paraffin-embedded tissue samples of different colorectal lesions [controls, chronic colitis, ulcerative colitis (UC), hyperplastic polyps (HPs), adenomas and CRCs] to investigate HER2 and SKP2 expression by immunohistochemistry (IHC), Selected CRC cases [equivocal (2+) and positive (3+) by IHC] were further evaluated by ISH (CISH and SISH ) to assess HER2 gene amplification. Results: Chronic colitis, UC, HPs and adenomas were HER2-negative. HER2 positivity (scores 2+ and 3+) was found only in15% of CRCs. Both SISH and CISH showed the same results with high concordance as 66.7% of equivocal and 100% of positive cases showed amplification of HER2 gene. SKP2 positivity was detected in 26.7% and 45% of adenomas and CRCs respectively, while other studied groups were negative. A significant correlation was noted between HER2 and SKP2 expression. Conclusion: A small percent of CRCs exhibited HER2 gene amplification, which would be potential candidates for anti HER2 therapy whereas IHC could be a primary screening test for patient selection. A potential carcinogenic role of SKP2 was suggested by the findings that SKP2 expression was undetectable in normal colonic mucosa but significantly increases from adenoma to carcinoma, hoping adenoma patients to get benefit from targeted therapy.
- انتشار مقاله: 24-02-1399
- نویسندگان: Mona Moussa,Afkar Badawy,Noha Helal,Fatma Hegab,Magdy Youssef,Tarek Aboushousha,Lubna Al Faruok,Dalal Elwy
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: bladder cancer,STAT3,CDK4
- چکیده:
- چکیده انگلیسی: Bladder cancer represents a global health problem. It ranks ninth in worldwide cancer incidence. In Egypt, carcinoma of the bladder is the most prevalent cancer, Bladder cancer has the highest recurrence rate of any malignancy. Certainly, suitable molecular diagnostic markers are required to improve the early detection of bladder cancer and then to prolong survival of patients. The present study was aimed to explore the expression of CDk4 and STAT3 in bladder cancer tissues as prospective for target therapy. Our studied groups showed higher values of CDK4 and STAT3 expression in malignant tissues (SCC andUC collectively) compared to cystitis, however, significantly higher values of CDK4 and STAT3 expression were detected in UC group compared to SCC group. Urothelial carcinomas with papillary patterns showed lower parameters of CDK4 and STAT3 expression compared to the non-papillary variant, with significant differences. Higher grades of UC showed significantly higher parameters of CDK4 and STAT3 expression compared to low grade ones. Muscle invasion increases the level of CDK4 and STAT3 expression parameters, compared to non-muscle invasive UC. Conclusion: Our results showed a good correlation of the expression patterns of both the cell cycle (CDK4) and inflammatory (STAT3) markers studied and might be helpful for suggesting more selective agents in the therapeutic scenario of bladder cancer in the near future. Potential biomarkers such as CDK4 andSTAT3 may be targets for molecular based therapeutic strategies in the prevention or management of bladder cancer. Future studies should explore molecular mechanisms of these proteins to define their roles in tumorigenesis.
- انتشار مقاله: 02-09-1398
- نویسندگان: Tarek Aboushousha,Olfat Hammam,Ahmed Aref,Amira Kamel,Mohamed Badawy,Amr Abdel Hamid
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Prostate,immunohistochemistry,RAGE,SOX2
- چکیده:
- چکیده انگلیسی: Background: Prostate cancer (PCa) is a common health problem in elderly. RAGE (Receptor for advanced glycation
end products) is overexpressed in multiple human cancers. SOX2 (Sex-determining region Y box 2) also functions as an
oncoprotein and promotes cancer progression but the mechanisms involved remain largely unknown. Aim: The current
study investigated the expression patterns of RAGE and SOX2 in benign and malignant prostate samples in correlation
with the histopathological findings in order to evaluate their role as prognostic markers or therapeutic targets. Methods:
Immunohistochemical staining for RAGE and SOX2 antibodies was applied on 87 prostatic biopsies [16 of prostatitis, 20
of benign prostatic hyperplasia (BPH) and 51 of PCa]. Results: Expression of RAGE and SOX2 (percentage of positive
cells) was significantly higher in PCa lesions compared with prostatitis (p<0.01) and BPH (p<0.0001) and was also
significantly higher in prostatitis compared with BPH lesions (p<0.01). Also, percentage of positive RAGE and SOX2
cells showed a significant stepwise increase from Gleason Grade 3 to Grade 5 and were significantly higher in high
Gleason Scores (≥8) compared to lower Scores (≤7) with statistical significance (p=0.001). Conclusion: RAGE and
SOX2 were up-regulated in prostate cancer lesions, mainly in advanced grades, suggesting an active role of both antigens
in the development and progression of prostate cancer and expecting the possibility of their use as therapeutic targets.- انتشار مقاله: 30-06-1397
- نویسندگان: Tarek Aboushousha,Rana Lashen,Khadega Abdelnaser,Noha Helal,Mona Moussa,Zeinab Omran,Samir Eldahshan,Hossam El Ganzoury
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Real-time PCR,Hepatocellular carcinoma,Osteopontin,Alpha-fetoprotein
- چکیده:
- چکیده انگلیسی: Background: Hepatocellular carcinoma (HCC) is a high incidence disease in Egypt with a poor prognosis and
survival. Biomarkers are important for diagnosis of HCC at an early stage. Osteopontin (OPN), a glycoprotein secreted by
macrophages, osteoblasts, and T cells, is also highly expressed in a variety of tumors, such as examples in the breast, colon,
and stomach. The present study aimed to correlate the serum level of OPN in HCV-positive hepatocellular carcinoma
patients, with OPN expression in tumor and non-tumor liver tissues in order to identify its efficacy as a biomarker
for diagnosis. Material and Methods: Out of total of 146 patients, 80 were selected for inclusion in the study. Blood
samples as well as specimens of tumor and non-tumor liver tissue were collected. In addition, blood samples from 20
healthy volunteers were obtained as controls. Serum OPN and alpha-fetoprotein (AFP) were evaluated by ELISA for
HCC and control groups. OPN and AFP gene expression were examined by real-time PCR, after homogenization and
DNA extraction from serum samples and liver tissues. Results: It was found that serum OPN levels were significantly
higher in the HCC group compared to normal group (P=0.009), with a strong positive correlation with AFP expression.
However, there was no significant difference between OPN expression in tumor and non-tumor liver tissue. Conclusion:
Serum OPN is highly suggested to be a professional candidate for HCC early diagnosis, with a diagnostic ability and
accuracy equal or higher than for AFP.- انتشار مقاله: 17-08-1396
- نویسندگان: Samah Mamdouh Abdel-Hafiz,Hussam EM Hamdy,Fatma M Khorshed,Tarek S Aboushousha,Gehan Safwat,Mohamed A Saber,Mohamed Seleem,Amira H Soliman
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: HCC,TTF1,RAGE,GLUT1,SOX2
- چکیده:
- چکیده انگلیسی:
Objective: To investigate the expression of TTF-1, RAGE, GLUT1 and SOX2 in HCV-associated HCCs and in surrounding non-tumorous liver tissue. Material and Methods: Tissue material from partial hepatectomy cases for HCC along with corresponding serum samples and 30 control serum samples from healthy volunteers were studied. Biopsies were classified into: non-tumor hepatic tissue (36 sections); HCC (33 sections) and liver cell dysplasia (LCD) (15 sections). All cases were positive for HCV. Immunohistochemistry (IHC), gene extraction and quantitative real-time reverse-transcription assays (qRT-PCR) were applied. Results: By IHC, LCD and HCC showed significantly high percentages of positive cases with all markers. SOX2 showed significant increase with higher HCC grades, while RAGE demonstrated an inverse relation and GLUT-1 and TTF-1 lacked any correlation. In nontumorous-HCV tissue, we found significantly high TTF-1, low RAGE and negative SOX2 expression. RAGE, GLUT-1 and SOX2 show non-significant elevation positivity in high grade HCV compared to low grade lesions. TTF-1, RAGE and SOX2 exhibited low expression in cirrhosis compared to fibrosis. Biochemical studies on serum and tissue extracts revealed significant down-regulation of RAGE, GLUT-1 and SOX2 genes, as well as significant up-regulation of the TTF-1 gene in HCC cases compared to controls. All studied genes show significant correlation with HCC grade. In non-tumor tissue, only TTF-1 gene expression had a significant correlation with the fibrosis score. Conclusion: Higher expression of TTF-1, RAGE, GLUT-1 and SOX2 in HCC and dysplasia compared to non-tumor tissues indicates up-regulation of these markers as early events during the development of HCV-associated HCC.- انتشار مقاله: 17-07-1396
- نویسندگان: Tarek Aboushousha,Samah Mamdouh,Hussam Hamdy,Noha Helal,Fatma Khorshed,Gehan Safwat,Mohamed Seleem
- مشاهده