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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: cervical cancer,gene polymorphism,XRCC1,APE-1,Base excision repair
- چکیده:
- چکیده انگلیسی: Backgrounds: Cervical cancer (CC) is one of the leading cause of death in women worldwide, HPV infection is the major risk factor in the disease development, 0and however other risk factor such as chemical carcinogens, genetic susceptibility and altered immune system are also a cause of the disease progression. In the light of the above statement we studied the base excision repair pathway (BER). Methods: We identified and studied the association of Single Nucleotide polymorphisms in the DNA repair genes of XRCC1 (Arg194Trp, Arg399G,) and APE-1Asp/148Glu to the susceptibility of cervical cancer (CC) in North Indian population. In our study of cases (n=102). Controls (n=109) were recruited from among women without cervical abnormalities. Genotypes were determined by PCR-CTPP method, Taking DNA from peripheral blood in a case control study. Results: A positive association was observed between the polymorphisms of XRCC1 genes, that is, in codons 194 (P=0.03, odds ratio (OR) =2.39, 95% confidence interval (CI)=5.2–1.1), 280 (P=0.01, OR=4.1, 95% CI=11.5–1.3) and 399 (P=0.01, OR=3.4, 95% CI=8.6–1.3) while APE-1 genotype GG (p=0.03,odds ratio(OR)=0.2,95% confidence interval (CI)=0.97-0.004) we observed a statistically significant protective role in developing cervical cancer. Conclusion: Our results suggested that, XRCC1 gene is an important candidate gene for susceptibility to cervical cancer. Although the sample size was small, the present study indicate a statistical association between cervical cancer and XRCC1 SNPs. Future studies are needed that may provide a better understanding of the association between gene polymorphism and cervical carcinoma risk.
- انتشار مقاله: 24-12-1398
- نویسندگان: Mark Rector Charles,Syed Tasleem Raza,Rolee Sharma,Pushpendra Pratap,Ale Eba,Manvendra Singh
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Prognosis,Real-time PCR,miRNA 335-5p,Gallbladder inflammatory diseases,Gallbladder cancer
- چکیده:
- چکیده انگلیسی: Background: MicroRNAs (miRNAs) are non-coding RNAs that regulate multiple cellular processes during cancer
progression, identified to be involved in tumorgenesis of several cancers including cancers of digestive system. However
its role in gallbladder inflammatory disease (GID) and gallbladder cancer (GBC) has not been well documented.
The present study was aimed to investigate the clinical significance of hsa-miRNA-335-5p (miR-335) in GBC and
GID. Subjects and Methods: This prospective case control study, conducted from July 1, 2014 to December 1, 2017
in Era’s Lucknow Medical College & Hospital, India, evaluated miR-335 expression by real-time polymerase chain
reaction. Hundred tissue samples GID (control; n=50) and GBC (case; n=50) were studied. Relative quantification of
target miR-335 expression was examined using the comparative cycle threshold method. Their expression was correlated
with different clinicopathological parameters. Fishers’ exact test, Student’s t-test, and Chi-square test were used as
appropriate for data analysis. Kaplan-Meier methods were used to calculate overall and disease-free survival rate.
Two sided PGBC lesions when compared with GID lesions (P<0.001). The low expression level of miR-335 was correlated with
histological grade (P=0.007), clinical stage (P<0.001), lymph node metastasis (P<0.001) and liver metastasis (P=0.016).
Reduced expression of miRNA-335 was associated with a shorter median overall survival (7 months vs. 25 months)
in GBC patients (P<0.001). Conclusions: Down regulation of miR-335 is associated with the severity of the disease
and thus indicate that miR-335 expression may serve as prognostic marker for GBC.- انتشار مقاله: 24-12-1397
- نویسندگان: Naseem Fatima,Anand Narain Srivastava,Jaya Nigam,Syed Tasleem Raza,Saliha Rizvi,Zainab Siddiqui,Vijay Kumar
- مشاهده