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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Ficus benghalensis, Ficus racemose, Antimicrobial,NDV,IBD
- چکیده:
- چکیده انگلیسی: Background and aims: Medicinal plant products are considered to be an effective candidate against the number of viral diseases as generally observed or reported in developing countries. As per the literature, secondary metabolites (i.e. Alkaloids, flavonoids, saponins etc.) are reported in medicinal plant products and showed its antiviral properties. In this study, our group focused on those medicinal plants especially roots of Ficus benghalensis and Ficus racemosa related to New castle Disease Virus (NDV) and Infectious Bursal Disease (IBD) having in vitro antiviral activity. These studies were conducted on the human peripheral blood mononuclear cells (PBMC).
Methods: For antimicrobial studies, different medicinal plant products especially roots of Ficus benghalensis and Ficus racemosa were collected from Vidya Pratishthan’s garden, School of Biotechnology, Baramati. These medicinal plant leaves are used in the form of aqueous extract and determined its anti-microbial activity against poultry viruses (i.e. NDV and IBD) in PBMC and determined proliferation assay, Th1 (TNF alpha) cytokine production and CD14 monocyte surface marker.
Results: Three medicinal plant aqueous extracts showed noteworthy antimicrobial activity with respect to decline in proliferation assay, TNF alpha production and CD14 monocyte surface marker in human PBMC as compared to control.
Conclusion: Some of the medicinal plant products have shown antimicrobial activity. Further immunobiological research is mandatory to elucidate the most active constituents that are present in these aqueous extracts which may be useful or target molecule in the development of new effective and safe antiviral agents.- انتشار مقاله: 05-04-1395
- نویسندگان: Amit Gupta,Sushama R Chaphalkar
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Immunosuppressive,Anti-inflammatory,Mangifera indica,Hepatitis B vaccine
- چکیده:
- چکیده انگلیسی: Background: There is a worldwide interest in searching for potential and effective medicinal plant candidates against various diseases or disorders. Till now, there are number of anti-inflammatory drugs like NSAIDs are available which showed various adverse effects in our body. To reduce these adverse effects, some of medicinal plants have been experimentally validated. Aim: The aim of our immunopharmacological study is to investigate the immunosuppressive and anti-inflammatory activity of aqueous leaves extract of Mangifera indica in human peripheral blood mononuclear cells (PBMC) extracellular against hepatitis B vaccine antigen (HBsAg) Methods: In this study, aqueous leaves extract were collected from Mangifera indica and determined its effect on total blood counts (using forward and side scatter) and monocyte CD14 FITC surface markers using flow cytometry. In addition, the effect of aqueous leaves extract on nitric oxide (NO) production from PBMC cell culture supernatant and also estimate its proliferation assay using Concanavalin A (Con A, 0.5 mg/ml, 50 µl). Data analysis was performed using BD cell Quest Pro software for flow cytometric analysis and one way ANOVA test (Boniferroni multiple comparison test). Results: The results displayed that aqueous leaves extract of Mangifera indica showed dose dependent decline in blood counts (increased in side scatter and slightly decreased in forward scatter), monocyte CD14 FITC surface marker; Con A proliferation and nitric oxide production from cell culture supernatant in human PBMC. Conclusion: Overall, the results claimed that aqueous leaves extract of Mangifera indica at higher doses showed immunosuppressive and anti-inflammatory activity.
- انتشار مقاله: 24-06-1394
- نویسندگان: Amit Gupta,Sushama R Chaphalkar
- مشاهده