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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Triple negative breast cancer,Cancer stem cells,Hippo signaling pathway,MDA-MB 231,quinacrine
- چکیده:
- چکیده انگلیسی: Objective: The Hippo signaling pathway has important role in the pathogenesis of some tumors. Breast cancer is the most prevalent cancer among females in the world. In recent years, various articles referred to inhibiting effect of quinacrine, a derivative of 9-aminoacridine, on the growth of several types of cancer cells. In this study, we evaluated the effect of quinacrine on expression of LATS1, LATS2, and YAP genes of the Hippo signaling pathway and YAP level in human breast cancer stem cells (MDA-MB 231 cell line). This cell line of breast cancer expresses the triple negative characteristics. Methods: MDA-MB 231 cells was treated with 0.5 µM of quinacrine for 3 days. The dose was selected using MTT assays. The expression of genes was quantified by Real-time PCR. The protein expression was performed by Western blotting. Significance of observations were checked by means of Mann-Whitney test using p
- انتشار مقاله: 13-12-1398
- نویسندگان: Soroush Darbankhales,Reza Mirfakhraie,Hossein Ghahremani,Mohsen Asadolahi,Kobra Saket-Kisomi,Lily Safakish,Sepideh Darbeheshti,Zahra Ganjkhanlou,Siamak Salami,Majid Sirati-Sabet
- مشاهده