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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Bioengineering Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Real-Time Polymerase Chain Reaction,Rotavirus,RNAs
- چکیده:
- چکیده انگلیسی: Introduction: We previously established virus like particles (VLPs) and core like particles (CLPs) of rotavirus (RV) with VP2, VP6, and VP7 proteins using stable high-five cell line. Given the fact that these proteins have the ability to assemble together, we further aimed to examine in detail the possibility of viral transcripts to randomly package in VLPs and CLPs.
Objective: For this purpose, the presence of VP2, VP6 and VP7 transcripts were evaluated in VLPs and CLPs.
Materials and Methods: To investigate the presence of VP2 transcript in CLPs of RV and VP2, VP6 and VP7 transcripts in RV VLPs, RNAs were extracted from VLPs and CLPs purified from stable high-five cells and evaluated by Real-time Reverse Transcriptase (RT)-Polymerase Chain Reaction (PCR).
Results: The result indicated that the RV VLPs and CLPs were empty (VP2, VP6 and VP7 transcripts-free), showing that VLPs and CLPs cannot encapsidate RNAs in absence of other viral proteins.
Conclusion: Our results indicate the necessity of further viral proteins for sorting and packaging, which highlight the role of selective mechanism for RV packaging.- انتشار مقاله: 30-10-1397
- نویسندگان: Zabihollah Shoja,Somayeh Jalilvand
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Bioengineering Research
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR,immunohistochemistry,Human herpesvirus 8,Kaposi’s sarcoma
- چکیده:
- چکیده انگلیسی: Introduction: Kaposi's sarcoma (KS) is a neoplasm involving blood and lymphatic vessels that has been characterized in four different clinical and epidemiological forms (classic, endemic, iatrogenic and epidemic). KS incidence is reported low in the general population of Iran while it is reported high among Iranian renal transplant recipients.
Objective: There are only some studies regarding the epidemiological and clinical features of KS in Iranian renal transplant recipients, but no reports are available on classic KS.
Materials and Methods: Present study intended to assess retrospectively the demographic and clinical features of 44 KS patients referred to the Razi Hospital in Tehran from 2000 to 2009. It also intended to detect human herpesvirus 8 to confirm the KS histological diagnosis.
Results: In these series of patients, 77.3% were male and 22.7% were female. There was a male predominance with a ratio of 3.4/1. Classic, epidemic and iatrogenic KS are found in 40 (90.9%), 2 (4.55%) and 2 (4.55%) of patients, respectively. The mean age was 62.87 among 40 patients with classic KS. In most cases (93.2%), KS was limited to the skin, without mucosal, lymph node or visceral manifestations. KS lesions mainly localized to the skin of extremities, particularly lower extremities. HHV-8 antigen and genome was detected in all samples.
Conclusion: This study showed that the picture of KS is similar to previous reports in our region. Also, our results confirmed the pathological diagnosis of KS cases in our study group.- انتشار مقاله: 12-10-1397
- نویسندگان: Somayeh Jalilvand,Zahra Safaie-Naraghi,Zabihollah Shoja
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: risk factors,Meta-Analysis,Nigeria,cervical cancer,human papillomavirus
- چکیده:
- چکیده انگلیسی: Background: Infection with an oncogenic type of human papillomavirus is a prerequisite for the development of precancerous cervical lesions and its subsequent progression to cervical cancer. With an alarming increase in the detection of other suspicious papillomavirus genotypes in both healthy and women with cervical lesions, there is a need for comprehensive data on cervical papillomavirus infection to address cervical cancer and other associated disease burden, especially in Sub-Sarahan Africa, where the bulk of the problem exists. The present study was conducted to develop comprehensive data on the prevalence and circulating genotypes of human papillomavirus in various risk categories in Nigeria. Methods: A systematic review and meta-analysis of peer-reviewed publications on cervical papillomavirus infection were performed. Relevant data were extracted from eligible studies published in PubMed, Web of Science, Embase, Scopus, and Google Scholar, from inception to July 31, 2019. The random-effect model was used to estimate the pooled prevalence. We identified 327 potential studies and pooled data from 18, involving 5697 women aged 15-86 years. Results: The overall pooled prevalence of cervical papillomavirus infection was 42% (95%CI: 30-54%) in the general population and 37% (95%CI: 25-50%) among women living with HIV/AIDs, with the predominance of genotypes 16, 18, 31, 35, 52, 58 and 45. The highest prevalence was observed in teenagers and young adults and the second peak in women 50 years and above. Conclusion: The prevalence of cervical human papillomavirus infection is cumulatively high in Nigeria and HIV is a strong co-factor. We, therefore, strongly recommend the co-screening of human papillomavirus and cervical cancer and integration of the intervention strategy into the existing HIV-care guideline in Nigeria.
- انتشار مقاله: 25-01-1399
- نویسندگان: Auwal Idris Kabuga,Ahmad Nejati,Amanuel Godana Arero,Somayeh Jalilvand,Talat Mokhtari-Azad,Shirin Shahbazi Sighaldeh,Umma Hassan Wali,Shohreh Shahmahmoodi,Mohamed E El Zowalaty
- مشاهده