در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Computational Applied Mechanics
- نوع مقاله: Journal Article
- کلمات کلیدی: Nanomedicine,Vortex Flow,Magnetic bar,Liquid height,Mono-dispersity
- چکیده:
- چکیده انگلیسی: One of the most common tools for fabricating different drug-loaded polymeric particles is magnetic stirrer, a widely-used tool in nano-based drug delivery systems. Typically, the revolutions per minute (rpm) or G-Force of the stirrer are reported in related literature, while other parameters generate less attention and must be better understood. Reporting the rpm or G-Force is likely insufficient for producing the same vortex flow intensity and mono-dispersity as having a reliable and reproducible nanoparticle and microparticle synthesis method. We speculate that the magnetic stirrer bar’s length and diameter, and the size of the cylindrical container, affect the qualities of nanoparticles and microparticles. Given the importance of these particle characteristics in the field of nanomedicine, understanding these details would improve reporting method reliability. These data are currently missing in most related papers and must be reported. The purpose of our study is to highlight the importance of these underestimated parameters (magnetic bar’s length, diameter, and the size of the cylindrical container) and the impact on the reproducibility of particle synthesis methods using a magnetic stirrer.
- انتشار مقاله: 05-04-1399
- نویسندگان: Narges Mahmoodi,Jafar Ai,Zahra Hassannejad,Somayeh Ebrahimi-Barough,Elham Hasanzadeh,Amin Hadi,Houra Nekounam,Vafa Rahimi-Movaghar
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Computational Applied Mechanics
- نوع مقاله: Journal Article
- کلمات کلیدی: Nanomedicine,Vortex Flow,Magnetic bar,Liquid height,Mono-dispersity
- چکیده:
- چکیده انگلیسی: One of the most common tools for fabricating different drug-loaded polymeric particles is magnetic stirrer, a widely-used tool in nano-based drug delivery systems. Typically, the revolutions per minute (rpm) or G-Force of the stirrer are reported in related literature, while other parameters generate less attention and must be better understood. Reporting the rpm or G-Force is likely insufficient for producing the same vortex flow intensity and mono-dispersity as having a reliable and reproducible nanoparticle and microparticle synthesis method. We speculate that the magnetic stirrer bar’s length and diameter, and the size of the cylindrical container, affect the qualities of nanoparticles and microparticles. Given the importance of these particle characteristics in the field of nanomedicine, understanding these details would improve reporting method reliability. These data are currently missing in most related papers and must be reported. The purpose of our study is to highlight the importance of these underestimated parameters (magnetic bar’s length, diameter, and the size of the cylindrical container) and the impact on the reproducibility of particle synthesis methods using a magnetic stirrer.
- انتشار مقاله: 05-04-1399
- نویسندگان: Narges Mahmoodi,Jafar Ai,Zahra Hassannejad,Somayeh Ebrahimi-Barough,Elham Hasanzadeh,Amin Hadi,Houra Nekounam,Vafa Rahimi-Movaghar
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,miRNAs,Glioblastoma,miR-4731
- چکیده:
- چکیده انگلیسی: GBM (Glioblastoma multiforme) is the most prevalent and lethal primary brain tumor. Gene therapy is one of the promising approaches and involves the delivery of genetic therapeutic molecules for specific antitumour response/activity. miRNAs can regulate the cell biology functions including replication, cell growth, and apoptosis by regulating gene expression. In this study, we found that down-regulation of miR-4731 expression occurred in GBM cells. We further determined that miR-4731 behaved as a tumor suppressor by inhibiting GBM cell proliferation. We further investigated the molecular mechanisms of miR-4731 and EGFR, ERK-1,2 and AKT-1,2 in GBM cell lines U87 and U251. The in vitro ectopic expression of miR-4731 affected cell proliferation, migration, and invasion of U87 and U251 cells. Luciferase reporter assays validated that miR-4731 targeted the 3′-untranslated region (3′-UTR) of EGFR. In conclusions, we identified that miR-4731 plays a tumor suppressor role in GBM cell proliferation and migration by targeting EGFR expression, and miR-4731 may act as a novel biomarker for early diagnosis or therapeutic target of GBM.
- انتشار مقاله: 05-10-1398
- نویسندگان: Amir Alahverdi,Ehsan Arefian,Masoud Soleimani,Jafar Ai,Aaliakbar Yousefi-Ahmadipour,Abouzar Babaei,Md Shahidul Islam,Somayeh Ebrahimi-Barough
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Glioblastoma,Angiogenesis,Atorvastatin
- چکیده:
- چکیده انگلیسی: Purpose: Glioblastoma multiform (GBM) is the most aggressive glial neoplasm. Researchers have exploited the
fact that GBMs are highly vascularized tumors. Anti-angiogenic strategies including those targeting VEGF pathway
have been emerged for treatment of GBM. Previously, we reported the anti-inflammatory effect of atorvastatin on
GBM cells. In this study, we investigated the anti-angiogenesis and apoptotic activity of atorvastatin on GBM cells.
Methods: Different concentrations of atorvastatin (1, 5, 10μM) were used on engineered three-dimensional (3D)
human tumor models using glioma spheroids and Human Umbilical Vein Endothelial cells (HUVECs) in fibrin gel
as tumor models. To reach for these aims, angiogenesis as tube-like structures sprouting of HUVECs were observed
after 24 hour treatment with different concentrations of atorvastatin into the 3-D fibrin matrix and we focused on it by
angiogenesis antibody array. After 48 hours exposing with different concentrations of atorvastatin, cell migration of
HUVECs were investigated. After 24 and 48 hours exposing with different concentrations of atorvastatin VEGF, CD31,
caspase-3 and Bcl-2 genes expression by real time PCR were assayed. Results: The results showed that atorvastatin
has potent anti-angiogenic effect and apoptosis inducing effect against glioma spheroids. Atorvastatin down-regulated
the expression of VEGF, CD31 and Bcl-2, and induced the expression of caspase-3 especially at 10μM concentration.
These effects are dose dependent. Conclusion: These results suggest that this biomimetic model with fibrin may provide
a vastly applicable 3D culture system to study the effect of anti-cancer drugs such as atorvastatin on tumor malignancy
in vitro and in vivo and atorvastatin could be used as agent for glioblastoma treatment.- انتشار مقاله: 25-10-1396
- نویسندگان: Neda Bayat,Reza Izadpanah,Somayeh Ebrahimi-Barough,Abbas Norouzi-Javidan,Arman Ai,Mohammad Mehdi Mokhtari Ardakan,Hooshang Saberi,Jafar Ai
- مشاهده