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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,hypermethylation,UNC5C,Netrin-1,DCC
- چکیده:
- چکیده انگلیسی: Background:The development of Colorectal Cancer (CRC) is a complex multistep process involving an accumulation
of multiple genetic and epigenetic alterations. Epigenetic modifications, particularly DNA methylation in selected
gene are recognized as common molecular alterations in human tumors. Netrin-1 receptors are aberrantly methylated
in primary colorectal cancer. Epigenetic alterations in the netrin-1 receptors have been found to be related with the
malignant potential of CRC. Purpose: In the present study, we evaluated the role of promoter hypermethylation
of UNC5C gene (one of the netrin-1 receptors) in colorectal cancer patients of Kashmiri population (North India).
Hypermethylation in tumour tissue was detected by Methylation- Specific Polymerase Chain Reaction (MS-PCR).
Results: UNC5C promoter hypermethylation was significantly found to be associated with colorectal cancer cases
where frequency was 62% (31 of 50) and 38% (19 of 50) patients were unmethylated (p<0.0001).UNC5C methylation
was significantly higher in CRCs with a frequency of 62% than 10% in corresponding normal mucosa of (p<0.0001).
Further, UNC5C hypermethylation was found to be significantly associated with stage-III/IV as compared to stage I/II
with a frequency of 75.8% and 42.8% respectively(p>0.05). Conclusion: We conclude that UNC5C hypermethylation
is implicated in CRC which plays a role in its tumorigenesis and may predict the late stage disease.- انتشار مقاله: 15-11-1395
- نویسندگان: Sartaj A Guroo,Ajaz A Malik,Dil Afroze,Shazia Ali,Arshad A Pandith,Adfar Yusuf
- مشاهده