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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Cattle,Buffaloes,Coombs’ test,IMHA
- چکیده:
- چکیده انگلیسی: The main objectives of this study were to determine the occurrence and potential causative factors of Immune-mediated hemolytic anemia (IMHA) in native cattle and water buffaloes from southwest of Iran. Fifty-three anemic animals (37 cattle and 16 buffaloes) were studied. A full clinical history and physical examinations were undertaken for all animals. Four clinically healthy cattle and four healthy buffaloes were also used as control animals. Blood samples were subjected to a complete blood count, Coombs’ test, erythrocyte osmotic fragility test and serum biochemical analysis. IMHA was diagnosed in 12 (32.43%) cattle and 6 (37.50%) buffaloes based on the Coombs’ test. Underlying or concurrent diseases, including theileriosis, anaplasmosis, vaccination, and pneumonia were detected in 11 cattle and four buffaloes. Primary or idiopathic IMHA was identified in one cattle and two buffaloes that their Coombs’ test was positive. Hematologic and biochemical findings in the cattle with IMHA included a nonregenerative anemia, leukopenia, thrombocytopenia, increased osmotic fragility, hyperbilirubinemia and elevated serum alkaline phosphatase, aspartate aminotransferase and lactate dehydrogenase activities. It can be concluded that IMHA occurs in a significant proportion of anemic cattle and river buffaloes in southwest of Iran. The occurrence of IMHA in both cattle and buffaloes is mostly secondary to infectious diseases especially theileriosis and anaplasmosis. Clarification of the mechanisms of primary or idiopathic and secondary IMHA in cattle and buffaloes require further studies.
- انتشار مقاله: 05-08-1395
- نویسندگان: Seyedeh Missagh Jalali,Masood Ghorbanpour,Mohammad Razi Jalali,Aria Rasooli,Pegah Safaie,Farideh Norvej,Imaneh Delavari
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Antioxidant,In vitro,Nitrite,Vitamin,Methemoglobin
- چکیده:
- چکیده انگلیسی: Nitrite intoxication occurs frequently in ruminants and equines. The most common treatment of this disorder is administration of 1% methylene blue, although the use of some antioxidant agents e.g. vitamins and complementary treatment may also be useful. The aim of this study was to evaluate the in vitro antioxidative effects of some vitamins on methemoglobinemia induced by sodium nitrite. For this purpose the blood sample of a healthy dairy cattle was pre-incubated with three different concentrations (5, 10, 20 mmol L-1) of each vitamin (E, C, B1, A and a combination of vitamin E and vitamin C) as antioxidant agent at 4 °C for 24 hours. A control group with normal saline instead of vitamin was applied. Then, all samples were treated with sodium nitrite (10 mmol L-1) as an oxidant agent for 10 minutes and the level of methemoglobin formation was measured spectrophoto-metrically. The results revealed that the level of methemoglobin decreased significantly (P < 0.05), when vitamin E (10 and 20 mmol L-1) and vitamin C (5 mmol L-1) was applied to the tests, separately. Vitamin C at the concentration of 20 mmol L-1, was not effective, but it even increased methemoglobin formation significantly. Combination of vitamin E and C was significantly effective at concentration 5 mmol L-1, but not at concentration 10 and 20 mmol L-1. Vitamin A and vitamin B1 were not effective in any concentration. It was concluded that vitamins especially vitamin C and E can reduce oxidative effects which induced methemoglobin formation in vitro and could be used as an alternative medication.
- انتشار مقاله: 08-10-1391
- نویسندگان: Nahid Atyabi,Seyedeh Parastoo Yasini,Seyedeh Missagh Jalali,Hamid Shaygan
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Insulin,Mesobuthus eupeus,Scorpion,Cortisol,endocrine system
- چکیده:
زمینه مطالعه: مزوبوتوس اوپئوس یک گونه عقرب بومی در جنوب غربی ایران است که مسئول اکثر موارد عقرب گزیدگی در استان خوزستان میباشد.
هدف: اثر سم عقرب مزوبوتوس اوپئوس بر هورمونهای متابولیک اصلی در موش صحرایی مورد بررسی قرار گرفت.
روش کار: 45 سر موش صحرایی نر آلبینو به 3 گروه مساوی تقسیم شدند: گروه 1 (کنترل): 5/0 میلیلیتر نرمال سالین به صورت داخلصفاقی دریافت کردند، گروه 2 و 3: سم عقرب مزوبوتوس اوپئوس به ترتیب با دوز 1 و 2 میلیگرم به ازای هر کیلوگرم به صورت داخل صفاقی دریافت نمودند. نمونهگیری در زمان 8، 24 و 48 ساعت پس از تزریق سم و یا سالین انجام گرفت.
نتایج: مقادیر تیروکسین (T4) و ترییدوتیرونین (T3) در هر دو گروه دریافت کننده سم (گروه 2 و 3) نسبت به گروه کنترل در همه زمانها به صورت وابسته به دوز به طور معنیداری پایینتر بود. همچنین کاهش معنیداری در میزان انسولین در هر دو گروه دریافت کننده سم در مقایسه با گروه کنترل در همه زمانها مشاهده گردید. غلظت گلوکاگن، کورتیزول و گلوکز در هر دو گروه دریافت کننده سم (گروه 2 و 3) در مقایسه با گروه کنترل پس از 8 و 24 ساعت بهطور معنیداری افزایش یافته بود.
نتیجهگیری نهایی: یافتههای این مطالعه نشان داد سم عقرب مزوبوتوس اوپئوس قادر به سرکوب ترشح هورمونهای متابولیک اصلی شامل T3 و T4 و انسولین و تحریک ترشح گلوکاگن و کورتیزول میباشد که در مجموع منجر به هیپرگلایسمی میشوند.- چکیده انگلیسی: BACKGROUND: Mesobuthus eupeus is an indigenous scorpion species in Southwest Iran which is respon- sible for the majority of scorpion sting cases in Khuzestan province.
OBJECTIVES: The effects of M. eupeus venom were investigated on the chief metabolic hormones in rats.
METHODS: A total of 45 Albino male rats were divided into 3 equal groups: group 1 (control): 0.5 ml normal saline was administered intraperitoneally (IP); group 2 and 3: M. eupeus venom was administered with a dose of 1 and 2 mg/kg IP, respectively. Sampling was performed at 8, 24, and 48 hours after venom/ saline injection.
RESULTS: The levels of thyroxine (T4) and triiodothyronine (T3) were significantly lower in both venom receiving groups (groups 2 and 3) than in the control group, dose-dependently, at all sampling times. There was a significant decrease in insulin level in both intoxicated groups compared to the control group at all sampling times. Glucagon, cortisol and subsequently glucose concentrations were significantly increased in both groups receiving the venom (groups 2 and 3) compared to the control group at 8 and 24 hours following envenomation.
CONCLUSIONS: The findings of this study indicate that M. eupeus venom can suppress the secretion of essential metabolic hormones including T3, T4, and insulin and stimulate the release of glucagon, and cor- tisol, leading to hyperglycemia.- انتشار مقاله: 13-03-1398
- نویسندگان: Mohammad Razi Jalali,Seyedeh Missagh Jalali,Hedieh Jafari,Mohammad Babakhan
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Mesobuthus eupeus,Venom,Chicken,Pathology,Hematology
- چکیده:
- چکیده انگلیسی: This study aimed to evaluate the clinical, histopathological and hematological effects of Mesobuthus eupeus venom on chicken organs. Adult chickens were subcutaneously injected with five doses of M. eupeus venom (0.5, 2, 5, 10 and 20 mg/kg; four chickens per each dose). Symptoms were recorded during the experiment and blood samples were collected for hematological analysis. Moreover, a complete necropsy was performed. After macroscopic examination, tissue samples were obtained from the liver, kidneys, heart, lungs, intestines and brain of the chickens three days after venom administration. In intravenous injection, lethal dose of the venom was determined at 15 mg/kg. The first clinical, pathological and hematological symptoms in envenomated chickens were observed at M. eupeus doses of 2, 5 and 0.5 mg/kg, respectively. Hematological examination revealed a reduction in lymphocyte count following experimental envenomation, which returned to the pre-experiment level in almost all the cases. On the other hand, heterophil count was found to increase during the experimental period. In addition, erythrocyte count and hematocrit level were stable at all the intervals. Pathological examination was indicative of severe pulmonary hemorrhage, pulmonary and cerebral edema, tubular necrosis of the kidneys, hemorrhage in kidneys and heart, hyaline thrombus and congestion of the liver. According to the results of this study, poultry are resistant to the toxic effects of M. eupeus venom.
- انتشار مقاله: 23-10-1394
- نویسندگان: Mohammad Khosravi,Mansour Mayahi,Seyedeh Missagh Jalali,Anahita Rezaie,Ahmad Taghavi Moghadam,Zohreh Hosseini,Seyedeh Kolsum Barzegar,Somayeh Azadmanesh
- مشاهده