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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Medicinal plants and By-product
- نوع مقاله: Journal Article
- کلمات کلیدی: essential oil,Yield,endangered species
- چکیده:
- چکیده انگلیسی: The Dracocephalum kotschyi Boiss.is a wild-growing flowering plant belonging to the Lamiaceae family and has been used as a medicinal herb for rheumatoid diseases, headaches, congestion, stomach disorders, liver treatment. In order to study the effects of dryland farming system on some agronomical and physiological traits of D. kotschyi, two separate experiments were conducted in three locations of, Mashad (Golmakan station), Quchan (5km in the northeast of city) and Bojnord (Sisab station) under normal irrigation and dryland farming systems in 2018. The collected data were combined analysis over three locations and mean comparisons were made using Tukey method. The results showed significant effects of locations for all of the traits except carbohydrate contents. Effect of farming system was also significant for all traits. Farming system by location interaction effects were significant for aerial fresh and dry weigh, 1000 seeds weight, proline, DPPH, flavonoid, protein, chlorophyll a, carotenoid, nitrogen and potassium. Result showed the location of Quchan had significantly higher mean values for many of traits followed by Mashad. In comparisons between two method of cultivations, the lower and higher values of plant height (20.6 vs. 25.3 cm), main branch (13.1 vs. 14.9 per plant), leaf area (38.9 vs. 48.3 mm2), flower number (14.6 vs. 23.1 per plant), fresh weight (295 vs. 888 g/plant), dry weight (80 vs. 244 g/plant), 1000 seeds weight (0.27 vs. 0.35 g), Protein (9.74 vs. 11.06%), chlorophyll a (7.47 vs. 8.19 mg g-1FW), carotenoid (0.08 vs. 0.11 mg g-1FW), nitrogen (1.43 vs. 1.77%) and phosphorus (13.89 vs. 20.79 ppm) were obtained in dryland and irrigation farming, respectively. In contrast, the higher and lower values of internode length (4.11 vs. 3.71 cm), proline (1.44 vs. 0.77 mg g-1FW), DPPH (0.39 vs. 0.19 mg g-1FW), phenol (0.83 vs. 0.67 mg g-1FW), flavonoid (0.60 vs. 0.47 mg g-1FW), carbohydrate (8.2 vs. 6.2 mg g-1FW), and potassium (4.2 vs. 3.7%) were obtained in dryland farming system.
- انتشار مقاله: 25-06-1398
- نویسندگان: Mahmoud Shaabani,Majid Azizi,Ali Ashraf Jafari,Seyed Hadi Mousavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Crocus sativus,Cytotoxicity,Saffron
- چکیده:
- چکیده انگلیسی: Current therapies for breast cancer are often limited by short-term efficacy due to the emergence of drug resistance. There has been increased interest in the use of naturally occurring compounds with chemopreventive and chemotherapeutic effects in the treatment of cancers. Saffron, dry stigmas Crocus sativus L., used in Iran as a spice, is known for its anticancer properties. In this study, the cytotoxic and apoptogenic effects of saffron in MCF-7 cells as an in vitro model for breast cancer study were investigated. Meanwhile role of caspases were studied in its toxicity. MCF-7 and L929 cell lines were cultured in DMEM medium and incubated with different concentrations of saffron extract (100 to 2000 μg/ml). Cell viability was assessed by MTT assay. Apoptotic cells were determined using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). Role of caspase were studied using the pan-caspase inhibitor, z-VAD-fmk. Saffron extract decreased cell viability in MCF-7 cells as a concentration- and time-dependent manner. Doses of saffron inhibited 50% cell growth (IC50) against MCF-7 was 400 μg/ml after 48 h of incubation. Saffron could induce apoptosis in MCF-7 cells in which apoptosis was dependent on caspase activation. It might be concluded that saffron could cause MCF-7 cell death, in which apoptosis or programmed cell death play an important role. Saffron could be also considered as a promising chemotherapeutic agent in breast cancer treatment.
- انتشار مقاله: 26-10-1385
- نویسندگان: Seyed Hadi Mousavi,Jalil Tavakkol Afsharic,Azam Brook
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,caspase,Lamiaceae,Salvia chorassanica
- چکیده:
- چکیده انگلیسی: Salvia chorassanica Bunge is one of the Iranian endemic species of Salvia. There is not any reported literature on S. chorassanica. This study was designed to examine the in-vitro anti-proliferative and proapoptotic effects of the methanol extract of S. chorassanica and its fractions on HeLa cell line. Cells were cultured in EX-CELL®, an animal free medium specially designed for HeLa cell line and incubated with different concentrations of plant extracts. Cell viability was quantified by MTS assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak). Activity of caspase -3, -8 and -9 was measured by the caspase colorimetric kit assay. S. chorassanica inhibited the growth of malignant cells and the CH2Cl2 fraction was determined as the most cytotoxic fraction in comparison with other fractions. The calculated IC50 values for methanol extract, n-hexane, CH2Cl2 and EtOAc fractions were 8.841, 5.45, 2.38, and 58.03 µg/mL, respectively. S. chorassanica induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that the cytotoxic mechanism is characterized by apoptosis induction. The activity of caspase-3 and 8 proteins in treated HeLa cells was significantly higher than that of the control while caspase-9 activity did not change significantly. Based on the result obtained from our study, the apoptosis pathway involved in S. chorassanica-induced cell death may be through the extrinsic pathway and it can be a novel promising candidate in the treatment of cancer.
- انتشار مقاله: 30-09-1390
- نویسندگان: Heydar Parsaee,Javad Asili,Seyed Hadi Mousavi,Hojjat Soofi,Seyed Ahmad Emami,Zahra Tayarani-Najaran
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Proliferation,Alendronate,Bisphosphonates,Human dental pulp stem cells
- چکیده:
- چکیده انگلیسی: Objective(s): Osteonecrosis of the jaw, as an exposed necrotic bone in the oral cavity, is one of the adverse effects of bisphosphonates, which have an affinity for bone minerals. This study investigates the cytotoxic effects of alendronate (ALN) as a nitrogen-containing bisphosphonate, on human dental pulp stem cells (hDPSCs).
Materials and Methods: The mesenchymal stem cells (MSCs), obtained from third molar tooth pulps were characterized by immunophenotyping assay in order to identify surface markers to evaluate their expression. To detect multipotency hDPSCs, they were differentiate into osteocytes and adipocytes. Cell proliferation was measured by MTT assay. PI staining of DNA fragmentation by flowcytometry (sub-G1 peak) was performed for determination of apoptotic cells and Bax, Bcl-2, and cleaved caspase 3 expressions. Protein expression was detected by Western blotting.
Results: As the results revealed, ALN decreased viable cells (in 0.8–100 µM) after 72 hr and 168 hr (P<0.001), significantly. ALN could lower cell proliferation in hDPSCs in a concentration and time-dependent manner. Sub-G1 peak as an indicator of flowcytometry histogram of treated cells by ALN, showed apoptosis was involved in ALN-induced cytotoxicity. Expressions of cleaved caspase 3 and Bax protein, as pro-apoptotic proteins, were increased and Bcl-2 protein as anti-apoptotic protein was decreased in response to increases in the concentration of ALN (0.8–25 µM).
Conclusion: Long-term effects of ALN on cell proliferation and apoptosis in hDPSCs can result in either initiation or potentiation of ALN-induced osteonecrosis.- انتشار مقاله: 19-01-1396
- نویسندگان: Solmaz Pourgonabadi,Ahmad Ghorbani,Zahra Tayarani Najaran,Seyed Hadi Mousavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Lipid Peroxidation,Saffron,Crocin,Acrylamide,Neurotoxicity,Gait score examination
- چکیده:
- چکیده انگلیسی: Objective(s):Acrylamide (ACR) has many applications in different industries. ACR damages the central and the peripheral nervous system in human and animals. Importance of ACR-induced neurotoxicity encouraged researchers to find both different mechanisms involved in ACR neurotoxicity and potent neuroprotective agents. Therefore, this study was designed to investigate the protective effect of crocin, an active constituent of Crocus sativus L. (saffron) on ACR-induced neurotoxicity in Wistar rats. Materials and Methods: Animals were treated with ACR (50 mg/kg, IP) 11 days for induction neurotoxicity. Crocin (12.5, 25 and 50 mg/kg, IP) were used during treatment with ACR. At the end of treatment, gait score examination was performed. Then, rats were sacrificed and the severity of damage in brain tissue was determined using pathological tests. The level of malondialdehyde (MDA) and glutathione (GSH) content were evaluated in cerebral cortex and cerebellum to determine the role of oxidative stress in this model. Results: Exposure to ACR induced severe gait abnormalities and pathological changes, but administration of crocin markedly improved behavioral index and histopathological damages. The elevation of lipid peroxidation parallel with reduction of GSH level was observed in cerebral cortex and cerebellum following exposure to ACR. Treatment with crocin markedly decreased MDA level, while elevated GSH content in nervous system as compared to ACR-treated animals. Conclusion: The administration of crocin markedly improved behavioral and histopathological damages in Wistar rats exposed to ACR. Reduction of oxidative stress can be considered as an important mechanism of neuroprotective effects of crocin against ACR-induced toxicity.
- انتشار مقاله: 13-07-1394
- نویسندگان: Soghra Mehri,Khalil Abnous,Alireza Khooei,Seyed Hadi Mousavi,Vahideh Motamed Shariaty,Hossein Hosseinzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Apoptosis,PC12,High glucose toxicity,Renin-angiotensin system
- چکیده:
- چکیده انگلیسی: Objective(s): Hyperglycemia, oxidative stress and apoptosis have key roles in pathogenesis of diabetic neuropathy. There are local renin-angiotensin systems (RASs) in different tissues such as neural tissue. Local RASs are involved in physiological and pathophysiological processes such as inflammation, proliferation and apoptosis. This study aimed to investigate the role of local renin-angiotensin system on high glucose-induced cell toxicity, apoptosis and reactive oxygen species (ROS) production in PC12 cells, as a cell model of diabetic neuropathy.
Materials and Methods: PC12 cells were exposed to a high glucose concentration (27 mg/ml), captopril (ACE inhibitor), telmisartan and losartan (AT1 antagonists), and also PD123319 (AT2 antagonist) were administered before and after induction of high glucose toxicity. Then cell viability was assessed by MTT assay and apoptotic cells and intracellular ROS production were detected by annexin V-propidium iodide and DCFDA, respectively, using flow cytometry.
Results: High glucose concentration decreased cell viability, and increased apoptotic cells. Intracellular ROS production was also increased. In PC12 cells pretreatment and treatment by the drugs showed a significant improvement in cell viability and reduced apoptosis in captopril, telmisartan and PD123319 but only captopril and telmisartan were able to reduce ROS production. Losrtan significantly lowered ROS but didn’t show any improvements in cell viability and apoptotic cells.
Conclusion: The results of the present study showed that RAS inhibitors reduced cell toxicity and apoptosis and ROS production was induced by high glucose. It may be suggested that local RAS has a role in high glucose toxicity.- انتشار مقاله: 05-06-1393
- نویسندگان: Kaveh Shahveisi,Seyed Hadi Mousavi,Mahmoud Hosseini,Abolfazl Khajavi Rad,Seyed Amir Jalali,Ziba Rajaei,Hamid Reza Sadeghnia,Mousa-Al-Reza Hadjzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Apoptosis,autoimmunity,Intrinsic/Extrinsic pathway,Neurodegenerative diseases
- چکیده:
- چکیده انگلیسی: Apoptosis or programmed cell death is a gene regulated phenomenon which is important in both physiological and pathological conditions. It is characterized by distinct morphological features including chromatin condensation, cell and nuclear shrinkage, membrane blebbing and oligonucleosomal DNA fragmentation. Although, two major apoptotic pathways including 1) the death receptor (extrinsic) and 2) mitochondrial (intrinsic) pathway have been identified, recently endoplasmic reticulum and lysosomal pathways have been also recognized. Depending on both the cell type and the initiating factor, distinct pathways are activated. The pathways share a common final phase of apoptosis, consisting of activation of the executioner caspases and dismantling of substrates critical for cell survival. The important regulatory mechanisms include death receptors, caspases, mitochondria and Bcl-2 family proteins. Modulating of apoptosis is a novel therapeutic strategy in treatment of different diseases. These include situations with unwanted cell accumulation (cancer) and failure to diminish aberrant cells (autoimmune diseases) or diseases with an inappropriate cell loss (heart failure, stroke, AIDS and neurodegenerative diseases). Modulation of apoptosis is a novel therapeutic strategy in treatment of different diseases. Many approaches including gene therapy, antisense strategies and numerous apoptotic drugs to target specific apoptotic regulators, are currently being developed. The goal of this review is to provide a general overview of current knowledge on the process of apoptosis including morphology, biochemistry, signaling as well as a discussion of apoptosis in diseases and effective therapy.
- انتشار مقاله: 14-07-1394
- نویسندگان: Seyed Hadi Mousavi,Zahra Tayarani-Najaran,Peter Hersey
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Hospital Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Knowledge,hematopoietic stem cell,Pregnant Women,Cord Blood Banking
- چکیده:
- چکیده انگلیسی: Background and Objectives: Nowadays, using umbilical cord blood (UCB) as a rich source of hematopoietic stem cells has been an important issue for both researchers and physicians in many parts of the world. According to the benefits of using cord blood in curing disorders, awareness of parents about cord blood utility and cord blood banking is important. The aim of the study was to evaluate the knowledge and willingness of Iranian pregnant women and also the information level of doctors about cord blood banking.
Methods: This is a survey that conducted among at least 28 weeks 824 pregnant women and 152 Obstetric-Gynecologists (OB-GYNs) in private hospitals of 24 provinces of Iran to evaluate the knowledge using a questionnaire.
Results: Our finding indicated that 38.7% of participants were aware of cord blood banking and less than 50% of doctors had complete information about cord blood usage. The mother’s attitude is related to their doctor’s recommendation. The results show that the level of education, employment status, and occupation, income has an impact on the parent’s attitude for cord blood banking.
Conclusion: The level of knowledge among the pregnant women about cord blood usage is low totally. Even the doctors had not complete awareness about cord blood banking. Hence the most of the women tend to get advice from their physician; we should try to introduce Royan cord blood bank and the advantages of cord blood stem cells via holding congresses and medical gathering.- انتشار مقاله: 18-09-1398
- نویسندگان: Bahareh Abbaspanah,Ashkan Mozdgir,Morteza Zarrabi,Saeed Abroun,Seyed Hadi Mousavi,Saeed Choolaei
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: GC/MS,oxidative stress,Glioblastoma,Dracocephalum kotschyi Boiss
- چکیده:
- چکیده انگلیسی: Objective: Glioblastoma multiforme (GBM) is the most aggressive and malignant brain tumor and has a poor prognosis. This study was aimed to investigate the cytotoxic effects of Dracocephalum kotschyi Boiss. (D. kotschyi) extracts in GBM U87 cell line.
Materials and Methods: The extracts of D. kotschyi obtained by two different ways of Soxhlet and soaked. The cytotoxic effects of D. kotschyi extracts were measured using MTT assay following treatment for different times of exposure (24, 48, and 72 hr) and at different concentrations of D. kotschyi extracts. The effects of D. kotschyi extracts on cellular oxidative stress were also evaluated by measuring cellular ROS levels. Furthermore, cellular death and apoptosis were studied by sub G1 analysis and Annexin V-FITC/propidium iodide (PI) staining using flow cytometry method, respectively. Characterization of the extracts was carried out using gas chromatography/mass spectrometry (GC/MS) analysis by Agilent GC-MSD system.
Results: Our results indicated thatD. kotschyi extracts decreased U87 cell viability in a time- and dose-dependent manner. Moreover, treatment with D. kotschyi extracted by Soxhlet for 24 and 48 hr significantly increased the levels of cellular ROS and Sub G1 population (p D. kotschyi mainly consisted of β-caryophellene, α-pinene and limonene.
Conclusion: Our findings demonstrated that D. kotschyi extracts can exert cytotoxic effects against GBM U87 cell line in a time- and concentration-dependent manner, and these effects may be mediated through intracellular ROS accumulating. However, further studies should be performed to confirm the efficacy and exact mechanism of action of the extracts.- انتشار مقاله: 13-06-1398
- نویسندگان: Mahmoud Shaabani,Seyed Hadi Mousavi,Majid Azizi,Ali Ashraf Jafari
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Cytotoxicity,Glioblastoma Multiforme,Brain tumors,Auraptene
- چکیده:
- چکیده انگلیسی: Objective: Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line.
Materials and Methods: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400μg/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide- stained nuclei. Moreover, to determine the Bax, Bcl-2, MCP-1, NF-κB, IL-1β, and p53 genes expression, we used real-time polymerase chain reaction (RT-PCR).
Results: The results revealed that auraptene reduced the viability of U87 cells concentration- and time-dependently with IC50 values of 108.9 and 79.17μg/ml obtained for 24 and 48-hr treatments, respectively. Also, sub-G1 population was significantly increased following 24 (p real-time RT-PCR showed an up-regulation in Bax, NF-κB, IL-1β, and p53 but a down-regulation in MCP-1 and Bcl-2 genes expression.
Conclusion: This study showed that auraptene triggered apoptosis probably through Bax/Bcl-2 regulation, blocked cell cycle progression and inhibited proliferation in U87 GBM cells. Taken together, auraptene can be utilized as an effective natural medicine against GBM, after complementary studies.- انتشار مقاله: 01-05-1397
- نویسندگان: Amir R. Afshari,Mostafa Karimi Roshan,Mohammad Soukhtanloo,Ahmad Ghorbani,Farzad Rahmani,Mohammad Jalili-nik,Mohammad Mahdi Vahedi,Azar Hoseini,Hamid R. Sadeghnia,Hamid Mollazadeh,Seyed Hadi Mousavi
- مشاهده