در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: sorafenib,Hepatocellular carcinoma,human placenta Mesenchymal stem cell,animal model
- چکیده:
- چکیده انگلیسی: Background: Hepatocellular carcinoma (HCC) is the fifth most diagnosed cancer and the third leading cause
of cancer-related death. sorafenib is used as a standard therapy to treat HCC. mesenchymal stromal cells (MSCs)
have also been used to suppress HCC. Here we investigate the development of a xenograft model of liver cancer to
study the homing of hpMSC-GFP cells, tumor kinetics and molecular characterizations of HCC. Methods: To create
xenograft models of HCC, HepG2 cell lines were inoculated into the flanks of 9 nude mice bilaterally. Animals were
then divided into three groups: the first group received hpMSC-GFP systemically, the second received intra-tumoral
hpMSC-GFP and the third received PBS. The first two groups were sacrificed after 72 hours of MSCs injection but
the third group was followed up for forty days. One tumor from each animal was then transferred to formalin buffer
for H&E staining and immunohistochemistry analysis (KI67 and CD34), and the other tumor was used for ex-vivo
imaging. Blood samples were taken from all subjects before sacrificing them. Results: Histopathological fidelity of
heterotopic HePG2 xenograft models to human HCC tumors was demonstrated. Biochemical evaluation suggested
the health of the animal’s liver and kidneys. Ex-vivo imaging illustrated homing of more hpMSC-GFP cells in tumor
tissues derived from the group receiving intra-tumoral hpMSC-GFP. Conclusion: A standard method was used to
inoculate tumor cells and the intervention was shown to be safe to liver and kidneys. Local injection of MSCs can be
used as cell therapy to fight neoplasms.- انتشار مقاله: 19-09-1396
- نویسندگان: Saieh Hajighasemlou,Saeedreza Pakzad,Jafar Ai,Samad Muhammadnejad,Milad Mirmoghtadaei,Faezeh Hosseinzadeh,Safoora Gharibzadeh,Amir Kamali,Akbar Ahmadi,Javad Verdi
- مشاهده