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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Herbal Drugs
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Minimum inhibitory concentration,Anti-Microbial activity,Anti-fungal activity,E. globules
- چکیده: مقدمه و هدف: در طی سالیان اخیر، بیماریهای عفونی ناشی از قارچهای فرصت طلب مانند Candida albicans و افزایش مقاومت باکتریهای بیماریای انسانی به آنتی بیوتیک های رایج منجر به بروز مشکلات زیادی در ارتباط با درمان این بیماری ها شده است. هدف از مطالعه حاضر بررسی اثرات ضد قارچی و باکتریایی عصاره گیاه اکالیپتوس بر علیه جدایه های Candida albicans جدا شده از نمونه های کلینیکی و تعدادی از جدایه های استاندارد از باکتری های بیماری زای انسانی بود.
روش تحقیق: عصاره اکایپتوس با استفاده از از دستگاه روتاری و روش خیساندن تهیه گردید. تعداد 30 جدایه از Candida albicans از بیماران مراجعه کننده به متخصص زنان و زایمان جدا شده و پس از خالص سازی به وسیله کلید های معتبر شناسایی شدند. در نهایت تعداد 30 جدایه برای بررسی اثر بازدارندگی رشد عصاره مورد استفاده قرار گرفتند. حداقل غلظت کشندگی و حداقل غلظت بازدارندگی عصاره اکالیپتوس علیه 5 سویه استاندارد باکتریایی شامل Staphylococcus aureu, Shigella dysenteriae, Listeria monocytogenes, Vibrio cholera and Bacillus cereusبا روش موردارزیابی قرار گرفت.
نتایج و بحث: نتایج ما نشان داد که در مقایسه با سویه های باکتریایی، جدایه های قرچی حساسیت بیشتری را نسبت به عصاره اکالیپتوس دارا می باشد. بیشترین و کمترین اثر کشندگی عصاره به ترتیب برای غلظت های 12.5 و 3.1 پی پی ام و علیه S. aureus و B. cereusثبت گردید. ماندد اثر کشندگی، بیشترین اثر بازدارندگی (20 پی پی ام) و کمترین اثر بازدارندگی (5پی پی ام) علیه S. aureus و B. cereusثبت گردید. حداقل غلظت کشندگی عصاره برای جلوگیری از رشد قارچی در غلظت 50 پی پی ام ثبت در حالی که بیشترین غلظت برای اثر بازدارندگی 150 پی پی ام ثبت گردید.
توصیه های کاربردی/ صنعتی: نتایج این مطالعه نشان داد که غلظت بالای اکالیپتوس قادر به بازدارندگی رشد قارچ Candida albicansمی باشد و در نتیجه مطالعات بیشتری در جهت آنالیز ترکیبات موثر در عصاره این گیاه می تواند انجام شود.- چکیده انگلیسی: Background & Aim:During recent decades, infections disease resulting from opportunistic fungi such as Candida albicans and resistance increasing of human pathogenic bacteria to current antibiotics has led to many problems for treating of these diseases. The aims of the present study was to investigate anti-fungi and anti-bacterial effects of Eucalyptus extract on Candida albicans species isolated from clinical samples and some standard human pathogenic bacteria.
Experimental: Eucalyptus extract was provided using rotary apparatus and maceration method. Thirty isolates of Candida albicans were isolated from referred patients to gynecologist and then were purified and identified by valid keys. Finally, 30 isolated were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of eucalyptus extract against 5 standard bacteria inculding: Staphylococcus aureu, Shigella dysenteriae, Listeria monocytogenes, Vibrio cholera and Bacillus cereus were evaluated using micro broth dilution method.
Results: Our results indicated that in compare to bacterial strains, fungus isolates showed more sensitivity to eucalyptus extract. The highest and lowest MIC of extract was recorded at 12.5 and 3.1 ppm for S. aureus and B. cereus, respectively. As MIC, the maximum MBC (20ppm) and minimum MBC (5ppm) of extract was recorded fro S. aureus and B. cereus respectively. Minimum inhibitory concentration of extract to fungal growth inhibitory was 50 ppm, whereas the maximum inhibiting concentration was 150 ppm.
Recommended applications/industries: The positive and interesting results suggest the essential oil of E. globulus could be exploited antibiotic for the treatment of candidiasous disease caused by Candida albicans fungi, and some human pathogenic bacteria studied in this work.- انتشار مقاله: 01-06-1396
- نویسندگان: Zahra Dehbashi,Forough Forghani,Seyed Kazem Sabbagh,Saeideh Saeidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Extract plant,Withania somnifera,Antifungal effects
- چکیده:
- چکیده انگلیسی: Background and aims: Withania somnifera (W. somnifera), commonly known as Ashwagandha, is an important medicinal plant that has been used in Ayurvedic and indigenous medicine for over 3,000 years. Candidiasis is one of the most common opportunistic fungal diseases in humans. In fact, the most important fungal disease in women is vaginal candidiasis. This study aimed to investigate the antifungal effects of the extracts of the W. somnifera on Candida albicans (C. albicans).
Methods: In this experimental research, 9 vaginal samples were collected using the sterile swap and Falcon tube by the gynecological specialists. The extracts of the shallot and artichoke were prepared using a rotary device. The inhibitory concentration against C. albicans was determined using incubation in media.
Results: The results of this study suggested that the minimum inhibitory concentration (MIC) against C. albicans, which is equivalent 50 ppm to 250 ppm has the highest concentration of inhibitor.
Conclusion: The results of this study showed that the antifungal activity of wind cheese against C. albicans is good, so it can be used as a drug to treat infections caused by C. albicans.- انتشار مقاله: 09-12-1394
- نویسندگان: Fereshteh Javadian,Zahra Sepehri,Saeideh Saeidi,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Artichoke,Candida albicans,Antifungal activity,Shallot
- چکیده:
- چکیده انگلیسی: Background and aims: Over the recent decades, the infections caused by the opportunistic fungus including the Candida albicans (C. albicans) have increased, thereby causing the high rate of diseases. With respect to the limitations of the treatment of the fungal infections and expensive antifungal drugs, researchers have been increasingly interested in finding anti-fungal drugs. This study aims to investigate the antifungal effects of the extracts of the shallots and artichokes on C. albicans.
Methods: In this experimental research, the extracts of the shallot and artichoke were prepared using a rotary device. The inhibitory concentration against C. albicans was determined using incubation in media.
Results: The results of the study showed that increased concentration of the extract increased the antifungal effects. The extract of the artichoke had higher inhibitory effect compared to the extract of the shallot. Moreover, as the extract was decreased the size of the fungi colony was increased.
Conclusion: The results of the study showed that extracts of the shallots and artichoke were proper inhibitors for the C. albicans and it is hoped that herbal medicines and possibly new compounds for treatment of the fungal infections be discovered in future.- انتشار مقاله: 16-10-1394
- نویسندگان: Mohammad Amin Mashhadi,Barat Ali Fakheri,Saeideh Saeidi
- مشاهده