در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Herbal Drugs
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,Plant extract,Datura Stramonium,Prosopis Farcta
- چکیده: مقدمه و هدف: هدف از این مطالعه بررسی فعالیت ضد میکروبی عصاره دو گیاه دارویی جغجغه و تاتوره بر روی استافیلوکوکوس اورئوس جدا شده از بینی گوسفندان در شهرستان زابل است.
روش تحقیق: گیاه تاتوره و جغجغه از مناطق شهر زابل جمع آوری شد. سویه های استافیلوکوکوس اورئوس از بینی گوسفندان شهرستان زابل جمع آوری شد. الگوی مقاومت آنتی بیوتیکی با روش کربی بائر تعیین شد. حداقل غلظت مهار کنندگی و حداقل غلظت کشندگی با روش میکرودایلوشن تعیین شد.
نتایج: نتایج مطالعه حاضر نشان داد که حداقل غلظت مهار کنندگی عصاره جغجغه برابر با ppm25 بوده است و 10 سویه در این غلظت مهار شده اند. کمترین غلظت مهار کنندگی عصاره تاتوره ppm 6.25 بوده و یک سویه در این غلظت مهار شد. بیشترین غلظت مهار کنندگی برابر با ppm 50 و بیشترین غلظت کشندگی ppm 100 مشاهده شد.
توصیه های کاربردی/ صنعتی: نتایج این مطالعه مبنی بر اثر ضد میکروبی خوب عصاره گیاه تاتوره و جغجغه نشان داد که میتواند برای درمان عفونت های استافیلوکوکی استفاده شود.- چکیده انگلیسی: Background & Aim: The aim of this study was to investigate the antimicrobial activity of two rangeland-medical plants extracts, Prosopis farcta L. and Datura stramonium L., against Staphylococcus aureus isolated from sheep in Zabol city.
Experimental: The P. farcta and D. stramonium were collected from the rangelands of Zabol city. Staphylococcus aureus strains were isolated from the nose of sheep in Zabol city. Antibiotic resistance pattern was determined by Kirby Bauer method. Finally, the minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) were determined by microdilution method.
Results: The results of this study showed that the P.farcta extract had a MIC of 25 ppm, and 10 strains of the bacterium were inhibited in this concentration. The lowest inhibitory concentration of D.stramonium extract was 6.25 ppm, and one strain was inhibited in this concentration. On the other hand, the highest inhibitory concentration was 50 ppm and the highest bactericide concentration was 100 ppm.
Recommended applications/ industries: The results of this study showed good antimicrobial effects of D. stramonium and P. farcta extracts that can be used to treat Staphylococcus aureus infections.- انتشار مقاله: 17-02-1397
- نویسندگان: Saeide Saeidi,Nafiseh Mahdi Nezhad,Majid Sharifi-Rad,Fereshteh Javadian,Elham Javadian
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Herbal Drugs
- نوع مقاله: Journal Article
- کلمات کلیدی: essential oil,Antimicrobial activity,Minimum inhibitory concentration,Zataria multiflora,Acinetobacter baumannii,Minimum bacterial concentration
- چکیده: مقدمه و هدف: هدف از این مطالعه بررسی فعالیت ضد میکروبی اسانس آویشن شیرازی بر روی اسینتو باکتر بومانی جدا شده از نمونه های بالینی است. روش تحقیق: دوازده سویه اسینتو باکتر بومانی از بیمارستان های شهرستان زابل جداسازی شد. اسانس آویشن شیرازی با استفاده از دستگاه کلونجر بدست آمد، در نهایت حداقل غلظت مهار کنندگی و حداقل غلظت کشندگی اسانس در برابر باکتریهای ذکرشده با روش میکرودایلوشن تعیین گردید. نتایج و بحث: نتایج حاصل از این مطالعه نشان داد که کمترین غلظت مهار کنندگی در برابر باکتری ها برابر با 31/0 میلی گرم بر میلی لیتر است و تنها یک سویه از باکتری در این غلظت مهار شده است. بیشترین غلظت مهار کننندگی برابر با 10 میلی گرم بر میلی لیتر بوده و یک سویه نیز در این غلظت مهار شده است. توصیه کاربردی و صنعتی: نتایج نشان داد که اسانس با افزایش غلظت اثرات ضد میکروبی آن افزایش پیدا می کند، اسانس آویشن فعالیت ضد میکروبی خوبی حتی در غلظت های پایین از خود نشان داد. با کاربرد اسانس آویشن شیرازی علیه باکتری های بیماریزا می توان به یک ماده ضد میکروبی خوب بدون اثر جانبی دست پیدا کرد.
- چکیده انگلیسی: Background & Aim: The aim of this study was to investigate the antimicrobial effects of Zataria multiflora against some Acinetobacter baumannii strains isolated from clinical samples. Experimental: Twelve strains of Acinetobacter baumannii were isolated from referred patients in Zabol hospital. Essential oil of Zataria multifloraspecies was extracted using Clevenger device. Minimum Inhibitory Concentration (MIC) and Minimum Bacterial Concentration (MBC) of essential oil on mentioned bacteria were determined using micro dilution broth method at six different concentrations. Results: The results of this study showed that the lowest inhibitory concentration of essential oil against bacteria was 0.31 mg/ml, and only one strain of bacteria was inhibited. However, the highest inhibitory concentration was estimated 10 mg/ml. Recommended applications/industries: The results showed that the antimicrobial effects increased with increasing in essential oil concentration and the essence showed good antimicrobial activity even at low concentrations. With the use of essential oil of Zataria multiflora against bacterial pathogens, a good antimicrobial agent can be obtained without any side effects.
- انتشار مقاله: 18-07-1396
- نویسندگان: Khadije Rezaie Keikhaie,Gholamreza Bagheri,Mehdi Hassanshahian,Saeide Saeidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Herbal Drugs
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Minimum inhibitory concentration,Anti-Microbial activity,Anti-fungal activity,E. globules
- چکیده: مقدمه و هدف: در طی سالیان اخیر، بیماریهای عفونی ناشی از قارچهای فرصت طلب مانند Candida albicans و افزایش مقاومت باکتریهای بیماریای انسانی به آنتی بیوتیک های رایج منجر به بروز مشکلات زیادی در ارتباط با درمان این بیماری ها شده است. هدف از مطالعه حاضر بررسی اثرات ضد قارچی و باکتریایی عصاره گیاه اکالیپتوس بر علیه جدایه های Candida albicans جدا شده از نمونه های کلینیکی و تعدادی از جدایه های استاندارد از باکتری های بیماری زای انسانی بود.
روش تحقیق: عصاره اکایپتوس با استفاده از از دستگاه روتاری و روش خیساندن تهیه گردید. تعداد 30 جدایه از Candida albicans از بیماران مراجعه کننده به متخصص زنان و زایمان جدا شده و پس از خالص سازی به وسیله کلید های معتبر شناسایی شدند. در نهایت تعداد 30 جدایه برای بررسی اثر بازدارندگی رشد عصاره مورد استفاده قرار گرفتند. حداقل غلظت کشندگی و حداقل غلظت بازدارندگی عصاره اکالیپتوس علیه 5 سویه استاندارد باکتریایی شامل Staphylococcus aureu, Shigella dysenteriae, Listeria monocytogenes, Vibrio cholera and Bacillus cereusبا روش موردارزیابی قرار گرفت.
نتایج و بحث: نتایج ما نشان داد که در مقایسه با سویه های باکتریایی، جدایه های قرچی حساسیت بیشتری را نسبت به عصاره اکالیپتوس دارا می باشد. بیشترین و کمترین اثر کشندگی عصاره به ترتیب برای غلظت های 12.5 و 3.1 پی پی ام و علیه S. aureus و B. cereusثبت گردید. ماندد اثر کشندگی، بیشترین اثر بازدارندگی (20 پی پی ام) و کمترین اثر بازدارندگی (5پی پی ام) علیه S. aureus و B. cereusثبت گردید. حداقل غلظت کشندگی عصاره برای جلوگیری از رشد قارچی در غلظت 50 پی پی ام ثبت در حالی که بیشترین غلظت برای اثر بازدارندگی 150 پی پی ام ثبت گردید.
توصیه های کاربردی/ صنعتی: نتایج این مطالعه نشان داد که غلظت بالای اکالیپتوس قادر به بازدارندگی رشد قارچ Candida albicansمی باشد و در نتیجه مطالعات بیشتری در جهت آنالیز ترکیبات موثر در عصاره این گیاه می تواند انجام شود.- چکیده انگلیسی: Background & Aim:During recent decades, infections disease resulting from opportunistic fungi such as Candida albicans and resistance increasing of human pathogenic bacteria to current antibiotics has led to many problems for treating of these diseases. The aims of the present study was to investigate anti-fungi and anti-bacterial effects of Eucalyptus extract on Candida albicans species isolated from clinical samples and some standard human pathogenic bacteria.
Experimental: Eucalyptus extract was provided using rotary apparatus and maceration method. Thirty isolates of Candida albicans were isolated from referred patients to gynecologist and then were purified and identified by valid keys. Finally, 30 isolated were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of eucalyptus extract against 5 standard bacteria inculding: Staphylococcus aureu, Shigella dysenteriae, Listeria monocytogenes, Vibrio cholera and Bacillus cereus were evaluated using micro broth dilution method.
Results: Our results indicated that in compare to bacterial strains, fungus isolates showed more sensitivity to eucalyptus extract. The highest and lowest MIC of extract was recorded at 12.5 and 3.1 ppm for S. aureus and B. cereus, respectively. As MIC, the maximum MBC (20ppm) and minimum MBC (5ppm) of extract was recorded fro S. aureus and B. cereus respectively. Minimum inhibitory concentration of extract to fungal growth inhibitory was 50 ppm, whereas the maximum inhibiting concentration was 150 ppm.
Recommended applications/industries: The positive and interesting results suggest the essential oil of E. globulus could be exploited antibiotic for the treatment of candidiasous disease caused by Candida albicans fungi, and some human pathogenic bacteria studied in this work.- انتشار مقاله: 01-06-1396
- نویسندگان: Zahra Dehbashi,Forough Forghani,Seyed Kazem Sabbagh,Saeideh Saeidi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Human pathogens,silver nanoparticles,Rosmarinus officinalis
- چکیده:
- چکیده انگلیسی: The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. The aim of this study was to determined antibacterial activity of silver nanoparticles produced by Rosmarinus officinalis L leaf extract against some human pathogenic bacteria. The formation and characterisation of AgNPs were confirmed by UV-Vis spectroscopy, energy-dispersive spectroscopy (EDX), X-ray diraction (XRD) and transmission electron microscope (TEM). All strains were obtained from standard laboratory and the minimum inhibitory concentrations were investigated by microdulition method.The result show that, the levels of MIC was observed ranges from 1.25 to 2.5 mg/ml. The highest MIC value was observed against S.pneumoniae, Hafnia alvei, S. saprophyticus.
- انتشار مقاله: 28-12-1394
- نویسندگان: Saeide Saeidi,Fereshteh Javadian,Zahra Sepehri,Zahra Shahi,Razieh Farazmand,Mahmood Anbari
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial activity,E. coli,Nano silver
- چکیده:
- چکیده انگلیسی: The aim study antibacterial effects of silver nanoparticles against resistant strains of E.coli bacteria.12 strains of E. coli strains arising urinary infection were isolated from hospitalized patient in zabolhospitals. Minimum inhibitory concentration of winter cherry were determined by dilution method in various concentration on bacteria. The highest MIC values was found to be 100ppm against one E.coli and the least MIC values was observed in 12.5 ppm. This work, integrates nanotechnology and bacteriology, leading to possible advances in the formulation of new types of bactericides.
- انتشار مقاله: 28-12-1394
- نویسندگان: Saeide Saeidi,Fereshteh Javadian,Zahra Sepehri,Zahra Shahi,Fahime Mousavi,Mahmood Anbari
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antimicrobial activity,Pseudomonas aeruginosa,Zinc oxide nanoparticles,Solanum nigrum
- چکیده:
- چکیده انگلیسی: Background and aims: The purpose of this study was to evaluate the antimicrobial effects of zinc oxide nanoparticale and extract of Solanum nigrum on Pseudomonas aeruginosa bacteria isolated from clinical specimens.
Methods: Zinc oxide was purchased from the market. 20g of the dried and pounded leaves of S. nigrum was used and its extract was prepared in rotary device. 12 isolates of P. aeruginosa were isolated from referred patients to hospital Zabol. Finally, 12 isolates were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of Zinc oxide and extract plant against P.aeruginosa were evaluated using micro broth dilution method.
Results:The highest inhibitory concentration for P. aeruginosa is 1500 μg/ml, with four bacterial strains being inoculated. Also, the results showed that the highest drainage concentration was 3000 μg/ml, which two strains were inhibited in this concentration and the lowest trap concentration was 93 μg/ml. The lowest inhibitory concentration of extract plant was 0.62 mg / ml, with only one strain being inhibited in this concentration. The highest inhibitory concentration for P. aeruginosa is 40 mg / ml, with four bacterial strains being inoculated.
Conclusion:The results showed that zinc oxide and extract of Solanum nigrum have a good antimicrobial activity on the bacterium and increases the concentration of antimicrobial activity.- انتشار مقاله: 18-07-1396
- نویسندگان: Khadijeh Rezaie.Keikhaie,Gholamreza Bagheri,Saeide Saeidi,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Extract plant,Withania somnifera,Antifungal effects
- چکیده:
- چکیده انگلیسی: Background and aims: Withania somnifera (W. somnifera), commonly known as Ashwagandha, is an important medicinal plant that has been used in Ayurvedic and indigenous medicine for over 3,000 years. Candidiasis is one of the most common opportunistic fungal diseases in humans. In fact, the most important fungal disease in women is vaginal candidiasis. This study aimed to investigate the antifungal effects of the extracts of the W. somnifera on Candida albicans (C. albicans).
Methods: In this experimental research, 9 vaginal samples were collected using the sterile swap and Falcon tube by the gynecological specialists. The extracts of the shallot and artichoke were prepared using a rotary device. The inhibitory concentration against C. albicans was determined using incubation in media.
Results: The results of this study suggested that the minimum inhibitory concentration (MIC) against C. albicans, which is equivalent 50 ppm to 250 ppm has the highest concentration of inhibitor.
Conclusion: The results of this study showed that the antifungal activity of wind cheese against C. albicans is good, so it can be used as a drug to treat infections caused by C. albicans.- انتشار مقاله: 09-12-1394
- نویسندگان: Fereshteh Javadian,Zahra Sepehri,Saeideh Saeidi,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Artichoke,Candida albicans,Antifungal activity,Shallot
- چکیده:
- چکیده انگلیسی: Background and aims: Over the recent decades, the infections caused by the opportunistic fungus including the Candida albicans (C. albicans) have increased, thereby causing the high rate of diseases. With respect to the limitations of the treatment of the fungal infections and expensive antifungal drugs, researchers have been increasingly interested in finding anti-fungal drugs. This study aims to investigate the antifungal effects of the extracts of the shallots and artichokes on C. albicans.
Methods: In this experimental research, the extracts of the shallot and artichoke were prepared using a rotary device. The inhibitory concentration against C. albicans was determined using incubation in media.
Results: The results of the study showed that increased concentration of the extract increased the antifungal effects. The extract of the artichoke had higher inhibitory effect compared to the extract of the shallot. Moreover, as the extract was decreased the size of the fungi colony was increased.
Conclusion: The results of the study showed that extracts of the shallots and artichoke were proper inhibitors for the C. albicans and it is hoped that herbal medicines and possibly new compounds for treatment of the fungal infections be discovered in future.- انتشار مقاله: 16-10-1394
- نویسندگان: Mohammad Amin Mashhadi,Barat Ali Fakheri,Saeideh Saeidi
- مشاهده