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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Oct4,luciferase,BMSC,P19,Post-transcriptional regulation,UTR
- چکیده:
- چکیده انگلیسی: Objective(s):OCT4 is a transcription factor required for pluripotency during early embryogenesis and the maintenance of identity of embryonic stem cells and pluripotent cells. Therefore, the effective expression regulation of this gene is highly critical. UTR regions are of great significance to gene regulation. In this study, we aimed to investigate the potential regulatory role played by 5´UTR and 3´UTR of the Oct4 gene in mouse BMSC and P19 cells.
Materials and Methods: The Oct4 5´UTR and 3´UTR sequences were cloned into pGL3 luciferase plasmid which led to the generation of pGL3 5´-UTR, pGL3 5´&3´-UTRs and pGL3 3´-UTR vectors. The vectors were transfected into BMSC and P19 cells followed by luciferase assay.
Results: The assay of luciferase expression exhibited a direct link between the presence of Oct4 3´- UTR and the decrease of luciferase count in both cell lines; whereas 5´UTR indicated diverse behaviors in two cells. This discrepancy could be explained in view of the difference of cellular contexts in which the Oct4 UTRs act.
Conclusion: This study sheds some light on the role of UTR regions of mouse Oct4 in regulating post-transcriptional gene expression in pluripotent cells. These data represent potential to be used for the development of novel therapeutic approaches for a variety of malignancies.- انتشار مقاله: 31-04-1393
- نویسندگان: Saeedeh Ghiasvand,Babak Bakhshinejad,Seyed Javad Mowla,Majid Sadeghizadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Morphine,Baclofen,Rats,Amygdala
- چکیده:
- چکیده انگلیسی: Background: The amygdala is one of the nerve centers involved in drug reward. It is suggested that the central nucleus of the amygdala (CeA) is involved in morphine dependency. The CeA gamma-aminobutyric acid-ergic (GABAergic) system is a mediator of morphine rewarding effects. In this research, the effects of stimulation or inhibition of CeA GABA type B (GABAB) receptors on sensitization acquisition to morphine-induced reward was evaluated in Wistar female rats using conditioned place preferential (CPP) method.Methods: Wistar female rats provided by Shahid Beheshti University, Tehran, Iran, were allocated into 17 groups including 7 groups of determining morphine dose-response, 2 groups of sensitivity and control, and 8 groups of different doses of agonists and antagonists in the acquisition stage (n = 7 in each group). Various quantities of morphine (0.5, 1, 2.5, 5, 7.5, 10 mg/kg of animal weight) were used to determine the effective and neutral doses of morphine. After 5 days from the start of the surgery, sensitization was induced. After the end of the sensitization period, CPP was conducted. Baclofen and CGP35348, as an agonist and antagonist of GABAB respectively, with the dose of 1.5, 6 and 12 μg/rat were inserted to the CeA, ten minutes before taking morphine.Findings: Administration of baclofen had no significant effect on the acquisition of morphine sensitization. In contrast, injection of CGP35348 reduced the sensitivity to morphine.Conclusion: GABA receptors can be effective in reducing morphine tendency by specific receptors, so these sites can be important therapeutic targets in counteracting the effects of drug abuse.
- انتشار مقاله: 30-06-1396
- نویسندگان: Firoozeh Alavian,Saeedeh Ghiasvand,Hedayat Sahraei,Maryam Rafiei-Rad
- مشاهده