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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Hippocampus,Treadmill Training,Female rats,Mir-1b,Sleep deprivation
- چکیده:
- چکیده انگلیسی: Objective(s): The protective effect of regular running on sleep deprivation (SD)-induced cognitive impairment has been revealed. In this study, we focused on the effects of regular exercise, sleep deprivation and both of them together on the microRNA-1b (miR-1b) expression and their relation to the behavioral parameters and brain-derived neurotrophic factor (BDNF) expression.
Materials and Methods: We used ovariectomized (OVX) female rats. The exercise program was mild-moderate treadmill training for 4 weeks. 72 hr SD was achieved using the multiple platform method and the spatial learning and memory parameters have been evaluated by the Morris water maze (MWM) test. The levels of studied genes were quantified by real-time PCR.
Results: SD down-regulated pri-miR-1b, miR-1b (P˂0.05), and BDNF mRNA (P˂0.01) in the hippocampus. Furthermore, female rats under exercise conditions showed significant up-regulation of the miR-1b and BDNF mRNA (P˂0.001). In addition, miR-1b positively correlated with cognitive function (P˂0.05) and BDNF mRNA (P˂0.01).
Conclusion: Our data demonstrated that regular treadmill exercise could reverse the down-regulation of hippocampal miR-1b, which has a probable role in the SD-induced cognitive impairment.- انتشار مقاله: 06-03-1397
- نویسندگان: Lily Mohammadipoor-ghasemabad,Mohammad Hossein Sangtarash,Saeed Esmaeili-Mahani,Vahid Sheibani,Hosein Ali Sasan
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Hippocampus,Treadmill Training,Female rats,Mir-1b,Sleep deprivation
- چکیده:
- چکیده انگلیسی: Objective(s): The protective effect of regular running on sleep deprivation (SD)-induced cognitive impairment has been revealed. In this study, we focused on the effects of regular exercise, sleep deprivation and both of them together on the microRNA-1b (miR-1b) expression and their relation to the behavioral parameters and brain-derived neurotrophic factor (BDNF) expression.
Materials and Methods: We used ovariectomized (OVX) female rats. The exercise program was mild-moderate treadmill training for 4 weeks. 72 hr SD was achieved using the multiple platform method and the spatial learning and memory parameters have been evaluated by the Morris water maze (MWM) test. The levels of studied genes were quantified by real-time PCR.
Results: SD down-regulated pri-miR-1b, miR-1b (P˂0.05), and BDNF mRNA (P˂0.01) in the hippocampus. Furthermore, female rats under exercise conditions showed significant up-regulation of the miR-1b and BDNF mRNA (P˂0.001). In addition, miR-1b positively correlated with cognitive function (P˂0.05) and BDNF mRNA (P˂0.01).
Conclusion: Our data demonstrated that regular treadmill exercise could reverse the down-regulation of hippocampal miR-1b, which has a probable role in the SD-induced cognitive impairment.- انتشار مقاله: 06-03-1397
- نویسندگان: Lily Mohammadipoor-ghasemabad,Mohammad Hossein Sangtarash,Saeed Esmaeili-Mahani,Vahid Sheibani,Hosein Ali Sasan
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rats,Allopregnanolone,Diabetic neuropathy,GABAA receptor,Hyperalgesia
- چکیده:
- چکیده انگلیسی: Objective(s):Painful diabetic neuropathy is associated with hyperexcitability and hyperactivity of spinal cord neurons. However, its underlying pathophysiological mechanisms have not been fully clarified. Induction of excitatory/inhibitory neurotransmission imbalance at the spinal cord seems to account for the abnormal neuronal activity in diabetes. Protective properties of neurosteroids have been demonstrated in numerous cellular and animal models of neurodegeneration.
Materials and Methods: Here, the protective effects of allopregnanolone, a neurosteroid were investigated in an in vivo model of diabetic neuropathy. The tail-flick test was used to assess the nociceptive threshold. Diabetes was induced by injection of 50 mg/kg (IP) streptozotocin. Seven weeks after the induction of diabetes, the dorsal half of the lumbar spinal cord was assayed for the expression of γ2 subunit of GABAA receptor using semiquantitative RT-PCR.
Results: The data shows that allopregnanolone (5 and 20 mg/kg) markedly ameliorated diabetes-induced thermal hyperalgesia and motor deficit. The weights of diabetic rats that received 5 and 20 mg/kg allopregnanolone did not significantly reduce during the time course of study. Furthermore, this neurosteroid could inhibit GABAA receptor down-regulation induced by diabetes in the rat spinal cord.
Conclusion: The data revealed that allopregnanolone has preventive effects against hyperglycemic-induced neuropathic pain and motor deficit which are related to the inhibition of GABAA receptor down-regulation.- انتشار مقاله: 30-02-1393
- نویسندگان: Samira Afrazi,Saeed Esmaeili-Mahani
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Morphine,Rats,ginger,p38 Mitogen-Activated Protein Kinases,Glial fibrillary acidic protein,Nucleus Accumbens
- چکیده:
- چکیده انگلیسی: Background: Chronic usage of morphine elicits the production of inflammatory factors by glial cells and
induces neuroinflammation. Ginger (Zingiber Officinale Roscoe) is a medicinal herb that has antiinflammatory properties. It has been reported that ginger shows anti-addictive effects against chronic usage
of morphine; however, its influence on morphine-induced neuroinflammation has not yet been clarified.
Methods: Morphine (12 mg/kg) was administrated intraperitoneally for 6 consecutive days. To evaluate the
effect of ginger on morphine-induced neuroinflammation, ginger extract (100 mg/kg) was given orally 30
minutes before morphine. Glial fibrillary acidic protein (GFAP) and P38 mitogen-activated protein kinase
(p38 MAPK) levels were assayed by immunoblotting in the rat nucleus accumbens (NAcc).
Findings: The injection of chronic morphine increased the levels of proteins involved in neuroinflammation
(p38 MAPK and GFAP) in NAcc. Furthermore, the levels of p38 MAPK and GFAP significantly returned to
the control levels by ginger extract.
Conclusion: The results suggest that the ginger extract can reduce morphine-induced neuroinflammation in NAcc.
- انتشار مقاله: 13-04-1398
- نویسندگان: Shima Torkzadeh-Mahani,Saeed Esmaeili-Mahani,Sima Nasri,Fatemeh Darvishzadeh,Reyhaneh Naderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Early life stress,Maternal deprivation,Addiction
- چکیده:
- چکیده انگلیسی: Maternal separation (MS) is defined as the termination of the continuity of mother-child relationship after the relationship is established. Although MS and maternal deprivation are different in terms of their definitions, these two terms are usually used interchangeably. This review aims to investigate the effect of MS on drug intake in adulthood. It has been proved that animal models are helpful in evaluating the effects of MS on drug intake risk in adulthood. There are relatively acceptable studies in this field on some drugs such as morphine, ethanol, and cocaine. However, very few animal studies, or even no animal study, have been conducted on some other drugs. The majority of these studies have considered MS as a risk factor for drug intake in adulthood. Different mechanisms are proposed for this phenomenon. Brain reward pathways are one of the main exploratory pathways of this process. Despite the importance of the issue, no human study with a specific concentration on investigating the relationship between MS and drug abuse in later life was found. Causal studies are warranted on humans to investigate the effect of MS on drug intake in later life.
- انتشار مقاله: 24-12-1394
- نویسندگان: Fatemeh Delavari,Vahid Sheibani,Saeed Esmaeili-Mahani,Nouzar Nakhaee
- مشاهده