در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Flow cytometry,MTT,Leishmania major,Pleurotus ostreatus
- چکیده:
- چکیده انگلیسی: Background and objectives: Leishmaniasis is caused by the genus Leishmania. Medications such as antimony compounds for the treatment of the disease are associated with limitations along with several side effects and disease recurrence; thus, evaluation of natural compounds with history of antimicrobial properties such as Pleurotus ostreatus, is of a great importance. The purpose of this study was to evaluate the apoptotic and leishmanicidal effects of Pleurotus ostreatus alcoholic extract on Leishmania major promastigote in vitro. Methods: Different concentrations of Pleurotus ostreatus extract (50, 100, 150, 200 and 250 μg/mL) were tested at 6, 24, 48 and 72 h on Leishmania major (MRHO/IR/75/ER) promastigotes. The leishmanicidal effects were determined using MTT [3-(4,5-dimethyl thiazolyl- 2)-2,5-diphenyle tetrazolium bromide] assay. Also, apoptosis induction was measured by flow cytometry and DNA fragmentation analysis. Results:The MTT results showed that leishmanicidal effect of Pleurotus ostreatus extract was dependent to extract concentration in a way that the lowest number of live promastigotes was obtained after treatment with 200 μg/mL of extract preparation at 72 h. The IC50 of Pleurotus ostreatus extract was 160±2 μg/mL. Flow cytometric analysis showed that the extract could induce apoptosis in promastigotes at its IC50. Also, the result of gel electrophoresis showed that DNA fragmentation of treated promastigotes at the same concentration. Conclusion: The results indicated that Pleurotus ostreatus alcoholic extract have a strong toxic effect on cultivated Leishmania parasites. Based on these results in vivo studies using rodent models and human cutaneous leishmaniasis CL is recommended.
- انتشار مقاله: 05-10-1395
- نویسندگان: P. Ramezani,S.H. Hejazi,M. Narimani,S. Soleimanifard
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Antigenic seed,Brucella abortus S99,Molecular test,Validation
- چکیده:
- چکیده انگلیسی: Brucellosis is a zoonotic infection that is associated with fever in humans and abortion in animals. The agent of this disease is a facultative intracellular gram-negative coccobacillus called Brucella. There are six classic species, including B. abortus, B. melitensis, B. suis, B. canis, B. neotomae, and B. ovis. In recent years, four new species have been reported, including Brucella ceti, B. microti, B. pinnipedialis, and B. inopinata. Human disease causes hygienic and economic losses, including inactivity of workforces in the community and high cost of treatment. The disease also causes catastrophic losses in the livestock industry. There is no effective vaccine against human brucellosis. Hence, attempts to prevent human infection with Brucella are focused on preventative measures, including control of infection in livestock, which lead to a reduction in its incidence in humans. The common methods for diagnosis of this disease are serologic methods including Rose Bengal, Wright -2 ME and the ring test. B. abortus strain S99 is used to produce these diagnostic antigens. The production of these antigens requires the presence of a well-characterized seed with full identity. The aim of this work was confirmation of the identity of B. abortus S99 by phage typing, AMOS and multiplex PCR techniques. Therefore, it is essential to carry out the identification of the strains used as seed for the production of the brucellosis diagnostic antigens. In this project, B. abortus strain 99 was supplied by the bacterial collection of the Brucellosis Department of Razi Vaccine and Serum Research Institute. Then, the main aim of the present study was the confirmation of the seed identity by doing the tests through the standard phage typing method, AMOS PCR and multiplex PCR (Brucladder) methods. Results were in support of the identity of the studied strain, and the molecular methods could also be used as the sensitive approaches for validation of antigenic seed.
- انتشار مقاله: 16-07-1397
- نویسندگان: S. Alamian,M. Dadar,S. Soleimani,A.M. Behrozikhah,A. Etemadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Quality Control,MMR,Stability study,vaccine,Potency test
- چکیده:
- چکیده انگلیسی: Stability studies play a critical role in assuring product quality at all points in the vaccine life cycle and have
a major impact on the success of immunization programs worldwide. The purpose of stability study is
determination of the vaccine quality under the variety of environmental factors and to establish a re-test
period or a shelf life and recommended storage conditions. In this study three batches of MMR vaccine of
Razi institute in Iran with AIK-C strain for measles, RS-12 strain for mumps and Takahashi strain for
rubella were tested for accelerated stability, after seven days incubation at 37 °C and for long-term stability
the samples were stored at 2-8 °C and tested intervals in three months until 36 months after production. All
of quantitative and qualitative control tests including Potency and Identity, Safety, Sterility, Mycoplasma
and Physicochemical tests were done in each period. In accelerated stability the mean loss of activity was
0.375, 0.373 and 0.210 log10 and in long-term stability the mean loss of activity was 0.626, 0.50 and 0.46
log10 for measles, mumps and rubella components of MMR vaccine respectively. In residual moisture test
the mean increase of moisture content in the vaccines was 1.084 %.In qualitative tests the vaccine met the
WHO specifications too. Results of this research indicated the MMR vaccines with these strains are stable
at least 36 months if the cold chain considered properly.- انتشار مقاله: 01-04-1394
- نویسندگان: S. Soleimani,S. Soleimani,S. Soleimani,S. Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Antigenic seed,Brucella abortus S99,Molecular test,Validation
- چکیده:
- چکیده انگلیسی: Brucellosis is a zoonotic infection that is associated with fever in humans and abortion in animals. The agent of this disease is a facultative intracellular gram-negative coccobacillus called Brucella. There are six classic species, including B. abortus, B. melitensis, B. suis, B. canis, B. neotomae, and B. ovis. In recent years, four new species have been reported, including Brucella ceti, B. microti, B. pinnipedialis, and B. inopinata. Human disease causes hygienic and economic losses, including inactivity of workforces in the community and high cost of treatment. The disease also causes catastrophic losses in the livestock industry. There is no effective vaccine against human brucellosis. Hence, attempts to prevent human infection with Brucella are focused on preventative measures, including control of infection in livestock, which lead to a reduction in its incidence in humans. The common methods for diagnosis of this disease are serologic methods including Rose Bengal, Wright -2 ME and the ring test. B. abortus strain S99 is used to produce these diagnostic antigens. The production of these antigens requires the presence of a well-characterized seed with full identity. The aim of this work was confirmation of the identity of B. abortus S99 by phage typing, AMOS and multiplex PCR techniques. Therefore, it is essential to carry out the identification of the strains used as seed for the production of the brucellosis diagnostic antigens. In this project, B. abortus strain 99 was supplied by the bacterial collection of the Brucellosis Department of Razi Vaccine and Serum Research Institute. Then, the main aim of the present study was the confirmation of the seed identity by doing the tests through the standard phage typing method, AMOS PCR and multiplex PCR (Brucladder) methods. Results were in support of the identity of the studied strain, and the molecular methods could also be used as the sensitive approaches for validation of antigenic seed.
- انتشار مقاله: 16-07-1397
- نویسندگان: S. Alamian,M. Dadar,S. Soleimani,A.M. Behrozikhah,A. Etemadi
- مشاهده