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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Surfactant,Cell culture,MTT assay,Solubility,Cosolvent
- چکیده:
- چکیده انگلیسی: Improving the solubility of non-soluble herbal materials is an issue of interest in cell culture based experiments. Evaluating the biological activity of these materials could become possible with the aid of cosolvents/surfactants which obviously should have little or no cytotoxic activity. In the present study, the cytotoxic activity of six cosolvents/surfactants: ethanol, methanol, Tween 20 and 80, propylene glycol (PG) and poly ethylene glycol 400 (PEG) which are usually helpful in dissolving non-soluble herbal extracts, has been evaluated against HepG-2, MCF-7 and HT-29 cells by MTT assay. Among the investigated cosolvents/surfactants, Tween 20 and 80 demonstrated the highest and ethanol and methanol the lowest cytotoxicity to the evaluated cell lines, suggesting the two latter as proper aids for improving solubility in biological experiments.
- انتشار مقاله: 09-11-1392
- نویسندگان: M. Hamzeloo-Moghadam,N. Taiebi,M. Mosaddegh,B. Eslami Tehrani,S. Esmaeili
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Medicinal plants,Ethnobotany,Hamedan
- چکیده:
- چکیده انگلیسی: A notable amount of medicinal plants grow in Iran and local communities in different parts of the country have developed a deep knowledge of various uses of plants during their old history. Several ethnobotanical studies have been conducted by the Traditional Medicine and Material Medica Research Center (TMRC) to investigate the use of medicinal plants by local people. Some of these studies have been carried out in Kohgiluye va Boyer Ahmad, Azarbayjan-e-Sharghi and Golestan provinces. These ethnobotanical data have been collected by semi-structured interviews. In the present study, two villages of Hamedan province were investigated and eleven informants were interviewed. Our results have presented 45 traditionally used plant species, belonging to 23 plant families and 39 genera. Asteraceae and Fabaceae were the dominant locally used families. The plants were used both for medicinal and non-medicinal purposes and the most treated problems were digestive disorders and infections.
- انتشار مقاله: 09-11-1392
- نویسندگان: Naghibi F.,S. Esmaeili,M. Malekmohammadi,A. Hassanpour,M. Mosaddegh
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytotoxicity,MTT assay,Jania adhaerens,Marine algae
- چکیده:
- چکیده انگلیسی: Iran has 1260 km of coastline that borders the Persian Gulf and the Oman Sea in the northwest Indian Ocean. Marine algae are one of the natural resources in the marine ecosystem which produce a wide range of new secondary metabolites with various biological activities that play an important role in the pharmaceutical care. In this study the cytotoxic activity of 28 marine algae of Chabahar coast was assessed against 5 cell lines including MCF-7, HepG-2, A-549, HT-29 and MDBK, through MTT assay. The methanol extract of the algae did not show cytotoxicity against any of the tested cell lines up to 100 μg/mL concentration, except for Jania adhaerens (IC50 85.03 µg/mL) against MCF-7 cells.
- انتشار مقاله: 04-10-1392
- نویسندگان: M. Mosaddegh,B.M. Gharanjik,F. Naghibi,S. Esmaeili,A. Pirani,B. Eslami Tehrani,B. Keramatian,A. Hassanpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Morphine,Nicotine,Leptin,NPY,Feeding behavior
- چکیده:
مطالعات قبلی هم در انسان و هم در حیوانات نشان دادهاند که تجویز نیکوتین سبب کاهش وزن و دریافت غذا میشود. از طرفی سیگار و تریاک به صورت سوء مصرف استفاده میشوند. هدف از مطالعه حاضر بررسی اثرات مصرف مزمن توام نیکوتین و مرفین بر روی میزان دریافت غذا، وزن بدن و بعضی از پپتیدهای تغذیهای میباشد. تمامی آزمایشها بر روی موش صحرایی نر نژاد ویستار انجام گرفته است. حیوانات به طور تصادفی در گروههای آزمایشی دریافت کننده غذای آزاد، غذای محدود، نیکوتین، مرفین، نیکوتین همراه با مرفین قرار گرفتند. سولفات مرفین (20 میلیگرم بر کیلوگرم برای 14 روز به صورت زیر جلدی) و نیکوتین (4 میلیگرم بر کیلوگرم برای 14 روز به صورت داخل صفاقی) به موشهای صحرایی تزریق شد. سطوح سرمی NPY و لپتین به ترتیب توسط سنجش رادیو ایمنی و سنجش رسوب ایمنی اندازهگیری شدند. نتایج حاکی از آن بود که نیکوتین اثرات کاهش دهندگی شدیدتری نسبت به مرفین به تنهایی و یا مرفین و نیکوتین توام در میزان کل دریافت غذا داشت. علاوه بر این در گروه تجویز مزمن نیکوتین سبب کاهش معنیداری وزن در طول تحقیق شد ولی در گروه دریافت کننده مرفین تغییر وزن رخ نداد. میانگین وزن حیوانات در گروه مرفینی کمتر از گروهی بود که غذای آزاد داشتند. فقط در گروهی که نیکوتین تجویز شده بود کاهش معنیداری در غلظت سرمی NPY رخ داد. کاهش قابل ملاحظهای در سطح سرمی لپتین در گروههای دریافت کننده دارو و غذای محدود نیز مشاهده شد. در نتیجه مرفین اثر کاهشی نیکوتین بر دریافت غذا را تعدیل میکند و به نظر میرسد بخشی از مکانیسم تعدیل اثرات نیکوتین توسط مرفین ناشی از جلوگیری از اثرات نیکوتین بر روی NPY بوده است.
- چکیده انگلیسی:
Previous studies in humans and animals have reported that nicotine administration decreases body weight
and caloric intake. Opiate and cigarette have been used concomitantly as drug abuse. The aim of the present study was, therefore, to analyze the effect of chronic co-administration of nicotine and morphine on food intake, body weight and on some feeding-associated peptides. All experiments were carried out on male Wistar rats. Animals were randomly assigned to the free-fed and pair-fed control groups, nicotine- and
morphine-treated and nicotine plus morphine groups. Morphine sulfate (20 mg/kg for 14 days s.c.) and
nicotine (4 mg/kg for 14 days i.p.) were injected to the rats. The serum levels of leptin and neuropeptide Y
(NPY) were measured by enzyme immunosorbant assay and radioimmunoassay, respectively. The results
showed that nicotine had a greater suppressing effect on total food intake than morphine alone or nicotine
plus morphine. Furthermore, chronic injection of nicotine significantly decreased body weight as compared
with before injection, while body weight changes were not observed in morphine-treated rats. The mean body
weight in the morphine-treated rats was lower than that in the free-fed control animals. The serum level of
NPY was decreased just in the nicotine-injected group. A significant decrease in leptin levels was observed
in the drug treated and pair-fed groups. In conclusion, morphine modulates the decreasing effect of nicotine
on food intake, and it seems that the mechanism underlying the attenuating effects of morphine on the
nicotine effects involves mediation, at least in part, by preventing the effect of nicotine on NPY levels.- انتشار مقاله: 07-02-1389
- نویسندگان: M. Rezvanipour,S. Esmaeili-Mahani,A. Siahposht,S. Rezvanipour
- مشاهده
- جایگاه : پژوهشی
- مجله: Caspian Journal of Environmental Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Pesticides,rice,Caspian coastline,Mineral elements
- چکیده:
- چکیده انگلیسی: Considering the increasing rice consumption in the world and also the excessive application of pesticides to increase production, an experiment was conducted to determine the pesticide residues and their effects on nitrogen, potassium and phosphorus content of Hashemi, Khazar and Gohar (SA13) rice cultivars, in a factorial design at randomized complete block design with three replications. In this study, rice plants were treated with three pesticides including insecticide Diazinon, herbicide Butachlor and fungicide Tricyclazole which are commonly used in the paddy fields of the southern coastline of the Caspian Sea, with standard concentrations recommended for these pesticides. The results indicated that the impacts of different pesticides on nitrogen, phosphorus and potassium contents in rice grains of Hashemi and Khazar cultivars were not significantly different. However, the nitrogen content reduced significantly in Gohar cultivar treated by Butachlor and Tricyclazole in comparison with control (p≤0.01). Determination of pesticides residues showed that Diazinon residue in white grains of Hashemi, Khazar and Gohar cultivars was lower than recommended limit determined by Codex (0.1 ppm), and also Tricyclazole amount was below the limit of detection. Therefore, using pesticides in permissible limits is strongly recommended. However, it cannot be concluded that using pesticides; even in permissible limits, does not have dangerous impacts over time on living organisms of the Caspian ecosystem.
- انتشار مقاله: 24-09-1393
- نویسندگان: M. Afshar Mohammadian,Z. Almasi,S. Esmaeili,F. Shakib,F. Majidi-Shilsar,M. Shokrzadeh-Lamuki
- مشاهده