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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Iranian Traditional Medicine,Malaria,Fever,heme detoxification
- چکیده:
- چکیده انگلیسی: Background and objectives: There are several methods to assess the in vitro capability of heme inhibitory activity of antimalarial compounds; most of them require some specific equipment or toxic substances and sometimes the needed materials are not accessible. Regarding the necessity and importance of optimizing and standardizing experimental conditions, the present study has intended to improve the in vitro assessment conditions of the β-hematin formation inhibitory activity for screening herbal samples. Methods: Hemin, tween 20, and samples (9:9:2) were incubated in different conditions including: hemin concentration (30, 60, and 120 µg/mL), duration (4, 24, 48, and 72 h), pH of buffer (3.6, 4, 4.4, 4.8, and 5), and temperature (37 and 60 °C) in 96-well plates. Also, a total of 165 plant extracts and fractions were tested in the most suitable conditions. Results: The reaction time and the incubation temperature were determined as the critical factors. The effective conditions for β-hematin formation were found to be 60 °C after 24 h incubation. In this method, proper correlations with respect to negative (69%) and positive (67%) predictive values were obtained in comparison with the anti-plasmodial assay. Antimalarial activities of Pistacia atlantica, Myrtus communis, Pterocarya fraxinifolia, and Satureja mutica were found to correlate significantly with inhibition of the heme detoxification assay. Conclusion: These results support a rapid, simple and reliable approach for selecting and identifying a number of herbs for further related antimalaria investigations.
- انتشار مقاله: 11-05-1396
- نویسندگان: M. Mosaddegh,M. Irani,S. Esmaeili*
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: DPPH,Antioxidant,FRAP,acetylcholinesterase,Kohgiluyeh va Boyerahmad
- چکیده:
- چکیده انگلیسی: Background and objectives:Alzheimer’s disease (AD) is a neurodegenerative disorder. Nowadays, many investigations are performed to find new drugs for AD and medicinal plants are considered as one of the most important sources for developing new drugs. According to the role of oxidant agents and acetylcholinesterase enzyme (AChE) in AD, plants with antioxidant and AChE inhibition properties could be good candidates for AD studies. In the present investigation, acetylcholinesterase inhibition (AChEI) and antioxidant effects of some plants from Kohgiluye va Boyerahmad province of Iran have been determined.
Methods: The plants collected from Kohgiluyeh va Boyerahmad province (56 species) were extracted with methanol by using maceration method. AChEI activity of the extracts was determined using Ellman method in 96-well microplates. Antioxidant activity was determined using DPPH and FRAP methods.
Results: The results showed that aerial parts of Amygdalus scoparia had the highest AChEI effect (50% inhibition in concentration of 300 µg/mL). The plant also demonstrated suitable antioxidant effects. Epilobium minutiflorum found to be the most potent species for DPPH inhibition and reduction of ferric-TPTZ complex (IC50 3.6 µg/mL and FRAP value 335.0 mmol FeSO4.7H2O/100g Extract).
Conclusion: Our results confirmed that almost all species with AChEI activity showed to be effective as potent antioxidant agents.
- انتشار مقاله: 05-04-1395
- نویسندگان: H. Hajimehdipoor,L. Ara,H. Moazzeni,S. Esmaeili
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Iranian Traditional Medicine,dry skin,qeirooti,skin fissure
- چکیده:
- چکیده انگلیسی: Dry skin is a common problem which affects wound healing, severity of other skin diseases and quality of life of people. One of its undesirable effects is fissure that is a cutaneous condition in which there is a linear loss of epidermis and dermis with sharply defined, nearly vertical walls. In the present study, we have investigated the etiology of the disease and its treatments in conventional medicine and Iranian Traditional Medicine (ITM). Two traditional medicine references, current scientific data bases and medicinal texts were explored with the selected keywords such as "sheqaq-e-poosti", "skin fissure" and "dry skin" to find the etiology and treatment of skin fissure. From the view point of both conventional medicine and ITM, dry skin is the cause of skin fissure and some causes of dry skin are similar. In conventional medicine, moisturizers are mainly used for treatment of dry skin; while in ITM some herbs, oils and other natural remedies have been used. A topical dosage form which was called "qeirooti", a mixture of wax and oil, was used to treat skin fissure in ITM. It comprised of oily ingredients that acted as occlusives and also some herbal components that directly improved dry skin (similar to moisturizers). Components efficacy of traditional dosage forms for treatment of dry skin lead us to study about formulation of “qeirooti” for treatment of dry skin.
- انتشار مقاله: 18-11-1393
- نویسندگان: A. Jedkareh,S. Esmaeili,A. Alembagheri,S.A. Mortazavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Thymus daenensis,Datura innoxia,Eryngium billardieri,Kohgiluyeh va Boyer Ahmad,Nerium indicum
- چکیده:
- چکیده انگلیسی: Background and objectives: Many people in ethnic groups of the world have trusted in plants for disease management and cure. Medicinal plants have always played a great role in the lives of Iranian people in the past and present and with no doubt in the future. Healers in different regions of Iran have been using medicinal herbs and one rich source for these cures is located in south-west of Iran, Kohgiluyeh va Boyer Ahmad province. Some species from this province have been selected for the present study and their cytotoxic activity has been evaluated. Methods: The methanol extracts of the 26 species were obtained by maceration and the extracts were investigated for cytotoxic activity in MTT assay. Results: The results revealed that four out of twenty six plants were toxic to MCF-7, A-549, HepG-2 and HT-29 cells. Conclusion: The findings of the present study specially the species with lower IC50 values (Eryngium billardieri and Nerium indicum) are suggested for further investigations in cancer studies.
- انتشار مقاله: 30-06-1394
- نویسندگان: S. Esmaeili,M. Irani,H. Moazzeni Zehan,B. Keramatian,Z. Tavakoli Harandi,M. Hamzeloo-Moghadam
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Traditional medicine,Ethnobotany,Hamedan,Alvand,Tuyserkan
- چکیده:
- چکیده انگلیسی: Background and objectives: Medicinal plants are widely used by people in the treatment of various diseases. These resources are usually regarded as part of cultural traditional knowledge. The aim of this study was to identify the information about the medicinal plants used by indigenous people of some regions of Hamedan province, Iran. Methods: The present ethnobotanical study was conducted in the Alvand mountainous area of Hamedan and Tuyserkan. Interviews were done in 27 villages and totally 53 informants were interviewed. Ethnobotanical indices like relative frequency of citation (RFC) and cultural importance index (CI) were calculated. Results: Our study reports 80 traditionally used plant species, belonging to 31 plant families. Asteraceae with 12 species was the most used family in this area. The most used parts were aerial parts (18.57%), leaves (21.42%), and flowers (17.14%), respectively. The most treated ailments were digestive problems. The highest number of ethnobotanical indices RFC and CI were observed in Stachys lavandulifolia Vahl. and Thymus lancifolius Celak., respectively. Conclusions: Hamedan provincepossesses considerable knowledge about medicinal plants for treating common health problems.
- انتشار مقاله: 29-07-1394
- نویسندگان: M. Mosaddegh,S. Esmaeili,A. Hassanpour,M. Malekmohammadi,F. Naghibi
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Alzheimer,Rosaceae,Acetylcholinesterase inhibition,Agrimonia eupatoria,Ellman’s method
- چکیده:
- چکیده انگلیسی: Background and objectives: Alzheimer's disease (AD) is an age dependent disorder. AD is associated with decrease of brain acetylcholine level. Nowadays, one of the methods for progression inhibition of AD is using acetylcholinesterase inhibitors. Rosaceae is a large plant family. Different biological effects of some species of this family have been reported. The aim of the present study was to assess the acetylcholinesterase inhibitory (AChEI) activity of the selected plants belonging to Rosaceae family. Methods: AChEI activity of six species from Rosaceae including Cotoneaster nummularia, Cerasus microcarpa, Amygdalus scoparia, Agrimonia eupatoria, Rosa canina and Rosa damascena were evaluated based on Ellman’s method in concentration of 300 µg/mL using total extracts and methanol fractions which were obtained by maceration. Results: The results showed that the total extract and methanol fraction of the aerial parts of A. eupatoria demonstrated significant AChEI activity with 46.5% and 56.2% inhibition of the enzyme, respectively. Conclusion: According to the results of the AChEI activity of the methanol fraction of A. eupatoria, it seems that the polar components of the species such as flavonoids may be responsible for its effectiveness.
- انتشار مقاله: 06-06-1394
- نویسندگان: S. Esmaeili,L. Ara,H. Hajimehdipoor,H. Kolivand,S. Mohammadi Motamed
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Anti-angiogenesis,wound healing assay,MTT assay,Crocus pallasii subsp. haussknechtii,HUV-EC-C
- چکیده:
- چکیده انگلیسی: Background and objectives: Angiogenesis is essential for tumor survival. Inhibiting angiogenesis could be a mechanism for hindering tumor development. Numerous studies have now been focused on agiogenesis inhibitors and many of such studies have targeted plant materials. In the present study, Crocus pallasii subsp. haussknechtii has been evaluated for anti-angiogenesis properties. Methods: Anti-angiogenesis activity of the plant extracts and fractions has been investigated through wound healing assay in HUV-EC-C cells. The cytotoxic activity has also been evaluated by MTT assay. Results: The methanol extract and the methanol fraction of the corm along with the chloroform fraction of the aerial parts demonstrated to be cytotoxic to HUV-EC-C cells with IC50 values of 27.2, 74.1 and 60.0 μg/mL, respectively while the chloroform fraction of the corm showed the most considerable anti-angiogenesis property among the samples in wound healing assay. Conclusion: Regarding the results of the present study, Crocus pallasii subsp. haussknechtii is suggested for further studies in cancer research evaluations.
- انتشار مقاله: 25-12-1393
- نویسندگان: M. Mosaddegh,S. Esmaeili,B. Eslami-Tehrani,B. Kermatian,S. Mohebby,M. Hamzeloo-Moghadam
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Medicinal plants,MTT,Iranian Traditional Medicine,Cytotoxic effect,Centaurea bruguierana
- چکیده:
- چکیده انگلیسی: Background and objectives: Iran owns a rich and prestigious heritage of medicinal herbs but the majority of these plants have not yet undergone chemical, pharmacological and toxicological studies. In the present study some species form northern parts of Iran were evaluated for cytotoxicity. Methods: Sixteen medicinal plants were extracted with methanol and screened for their cytotoxic activities. The inhibition of cell growth for these extracts was evaluated against MCF-7, WEHI-164, HepG-2 and MDBK cell lines. Their 50% inhibitions of growth (IC50) were determined by MTT assay. Moreover, cytotoxic evaluation of different fractions of the most potent species was performed. Results:Among examined samples, the IC50 values of methanol extract of Centaurea bruguierana (DC.) Hand.-Mzt. on mentioned cell lines were found 47.30-87.40 µg/mL. In addition, the chloroform fraction of the species was cytotoxic with IC50 values 17.00-23.03 µg/mL. Conclusion: It was concluded that the chloroform fraction of C. bruguierana was the best candidate for identification and isolation of active principles with cytotoxic effects. These results recommend further studies about this species.
- انتشار مقاله: 23-09-1393
- نویسندگان: S. Ghafari,F. Naghibi,S. Esmaeili,S. Sahranavard,M. Mosaddegh
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Medicinal plants,MTT assay,Cytotoxic activity
- چکیده:
- چکیده انگلیسی: Background and objectives: Cancer is a public health problem all over the world. Herbal medicines have a vital role in the prevention and treatment of cancer and large numbers of plants and their isolated constituents have shown potential anticancer activity. Methods: Twenty seven medicinal plants from southern Iran provinces have been extracted with methanol and screened for their cytotoxic activity against MCF-7, WEHI-164, HepG-2, MDBK and A-549 cell lines by MTT assay. Results: The methanol extracts of two species, Calotropis procera andJuniperus excelsa, demonstrated to be more effective compared to other extracts. Conclusion: The above species are proper candidates for further cancer studies.
- انتشار مقاله: 31-04-1393
- نویسندگان: S. Esmaeili,M. Hamzeloo-Moghadam,S. Ghaffari,M. Mosaddegh
- مشاهده
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Ethnopharmacology,Escherichia coli K-12(λ),inductest,Kohgiluyeh va Boyerahmad
- چکیده:
- چکیده انگلیسی: Background and objectives: There is a growing trend towards investigating natural products as sources of compounds with biological effects and many researches have been carried out in order to find effective medications against many diseases. Cancer is no exception and studies focusing on evaluating the effects of different materials on DNA, give valuable information in cancer researches and carcinogenicity studies; thus the present study was focused on evaluating the impact of medicinal plants from Kohgiluyeh va Boyerahmad province, Iran on DNA. Methods: Thirty five plant species collected have been investigated for prophage induction ability in Escherichia coli K-12(λ)throughinductest. Results:The assay demonstrated that 8 plants were able to affect DNA. Conclusion: The results confirm the role of natural resources for biologic effects and what’s more, potential drug candidates in new drug discovery.
- انتشار مقاله: 01-04-1393
- نویسندگان: M. Hamzeloo-Moghadam,B. Eslami Tehrani,A. Pirani,A. Hassanpour,S. Esmaeili
- مشاهده