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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: cervical cancer,gene polymorphism,XRCC1,APE-1,Base excision repair
- چکیده:
- چکیده انگلیسی: Backgrounds: Cervical cancer (CC) is one of the leading cause of death in women worldwide, HPV infection is the major risk factor in the disease development, 0and however other risk factor such as chemical carcinogens, genetic susceptibility and altered immune system are also a cause of the disease progression. In the light of the above statement we studied the base excision repair pathway (BER). Methods: We identified and studied the association of Single Nucleotide polymorphisms in the DNA repair genes of XRCC1 (Arg194Trp, Arg399G,) and APE-1Asp/148Glu to the susceptibility of cervical cancer (CC) in North Indian population. In our study of cases (n=102). Controls (n=109) were recruited from among women without cervical abnormalities. Genotypes were determined by PCR-CTPP method, Taking DNA from peripheral blood in a case control study. Results: A positive association was observed between the polymorphisms of XRCC1 genes, that is, in codons 194 (P=0.03, odds ratio (OR) =2.39, 95% confidence interval (CI)=5.2–1.1), 280 (P=0.01, OR=4.1, 95% CI=11.5–1.3) and 399 (P=0.01, OR=3.4, 95% CI=8.6–1.3) while APE-1 genotype GG (p=0.03,odds ratio(OR)=0.2,95% confidence interval (CI)=0.97-0.004) we observed a statistically significant protective role in developing cervical cancer. Conclusion: Our results suggested that, XRCC1 gene is an important candidate gene for susceptibility to cervical cancer. Although the sample size was small, the present study indicate a statistical association between cervical cancer and XRCC1 SNPs. Future studies are needed that may provide a better understanding of the association between gene polymorphism and cervical carcinoma risk.
- انتشار مقاله: 24-12-1398
- نویسندگان: Mark Rector Charles,Syed Tasleem Raza,Rolee Sharma,Pushpendra Pratap,Ale Eba,Manvendra Singh
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Circulating miRNA,Non-invassive biomarker,miR21
- چکیده:
- چکیده انگلیسی: Background: Circulating miRNAs (miRs) in the biofluids such as serum and plasma act as potential biomarkers
for early diagnosis, treatment and prognosis. In the present study, an attempt made to see the expression of miR-21
in serum of 20 cases of Oral sub-mucous fibrosis (OSMF), 20 cases of Oral squamous cell carcinoma and 40 healthy
volunteers. The expression of miR-21 was evaluated in relation to different demographical and clinicopathological
features such as sex, tobacco, pan-masala, alcohol, smoking and clinical staging respectively with an aim to identify
correlation with oral pre-cancer and cancer stages. Materials and Methods: The relative expression level of miR-21
was determined by quantitative real-time RT-PCR (qRT-PCR) in the sera of 20 OSCC, 20 OSMF patients and 40
healthy subjects as a control. Association between expression of miR-21 and OSCC clinical stages and demographical
parameters such as sex, pan-masala, tobacco, smoking, alcohol have also been analyzed in detail. Results: The results
obtained by t-test revealed significant increase in the expression level of miR-21 in OSCC as compared to OSMF. The
study also revealed the positive correlation between higher miR-21 expression and pan-masala chewers as shown by
t-test. The statistical test, ANOVA has also indicated a positive correlation between up-regulation of miR-21 in the
clinical stages of the OSCC. Conclusion: The results of present study indicated up-regulation of circulating miR-21 in
serum of OSCC as compared to OSMF (p=0.001), this study also elucidated the positive correlation between miR-21
expression in OSCC/OSMF patients, only one demographical parameter (Pan-masala) and negative correlation for
other parameters such as sex, tobacco, smoking, alcohol etc. Other findings suggested a significant increase (p=0.000)
in the expression of miR-21 in clinical staging (I-IV) of oral cancer. More studies are needed to validate it as potential
diagnostic and prognostic biomarker for OSMF and OSCC for better management.- انتشار مقاله: 17-09-1396
- نویسندگان: Pooja Singh,Anand Narain Srivastava,Rolee Sharma,Saboor Mateen,Bharat Shukla,Alok Singh,Siddhartha Chandel
- مشاهده