در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Radiotherapy,Cytotoxicity,Angiogenesis,Hyperthermia
- چکیده:
- چکیده انگلیسی: Background: There is no doubt that hyperthermia is one of the powerful radiosensitizers. Finding a proper mechanism
working in hyperthermia/radiation combination is still pronounced challenge. Objectives: This study is focusing on
the anti-cancer activities (anti-proliferative, anti-angiogenic and antiapoptotic) of thermoradiotherapy. Materials and
Methods: Liver cancer cell line (HepG2) was treated by 37oC, 40oC and 43oC hyperthermia degrees combined with
three radiation doses (2 Gy, 4 Gy and 8 Gy) for 24, 48 and 72 hrs. Cell viability, apoptotic/necrotic cell screening,
apoptotic (BAX and FasL) and antiapoptotic (BCL-2 and GRP78) genes, and pro-angiogenic mediators [vascular
endothelial- (VEGF) and Platelet derived-growth factors (PDGF) ware investigated. Results: Our data showed that 40oC
temperature combined with 4 Gy radiation gives a significant decrease (p<0.05) in cell viability. Maximum cytotoxicity
was reported 48 hr post-treatment followed by slight restoration of cell viability after 72 hr. Compared with untreated
cells, only 5% of viable cells with a high percentage of apoptotic (31%) and necrotic (63%) cells were demonstrated
in 40oC/4 Gy/48 hr group. Expression of pro-apoptotic genes (BAX and FasL) were increased after hyperthermia with
apparent elevation in 40oC/4 Gy/48 hr group coincides with moderate expression of antiapoptotic BCL-2 and GRP78
genes. A significant reduction (p<0.001; p<0.05) in VEGF and PDGF levels; respectively was shown at 40oC/4 Gy/48
hr group. Conclusions: This pilot study proposed 40oC mild temperature hyperthermia as a favorable hyperthermal
condition with 4 Gy radiotherapy in HCC treatment. A further research has to be performed considering an application
of more than one session of radiothermal therapy at 40oC/4 Gy for total abrogation of cancer cells.- انتشار مقاله: 05-09-1397
- نویسندگان: Roba M Talaat,Tamer M Abo-Zeid,Mahmoud T Abo-Elfadl,Eman A El-Maadawy,Mona M Hassanin
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Polymorphism,ALL,MDR-1,Egyptian
- چکیده:
- چکیده انگلیسی: Background: P-glycoprotein (P-gp), a membrane transporter encoded by the multidrug resistance-1 (MDR1) gene,
influences pharmacokinetics and metabolism of anticancer drugs and contributes to multidrug resistance phenotype
in acute lymphoblastic leukemia (ALL). Genetic variation ofMDR1 in ALL patients is increasingly recognized as
a factor influencing response to treatment. Aim: To investigate the possible role of MDR-1 gene polymorphisms
(C3435T, C1236T and C4125A) as risk factors for the development and clinical outcome of ALL in Egyptian children.
Materials and Methods: Genotyping of MDR-1 C3435T, C1236T and C4125A single nucleotide polymorphisms
(SNPs) was accomplished using a polymerase chain reaction–restriction fragment length polymorphism (RFLP-PCR)
assay with 120 childhood ALL patients and 100 healthy controls. Results: Homozygous T with the C3435T SNP showed
a protective effect as compared to homozygous C (OR=0.748) while heterozygous CT correlated with a poor outcome
(high risk, drug unresponsiveness, relapse and high percentage of death). Additionally, the T allele of the C1236T SNP
showed a significant relation with ALL risk (OR=1.6). However, there were no significant differences in the genotype
and allele frequencies of MDR-1 SNPs between patients and controls. Only one genotype (CC) and one allele of
MDR-1 (C4125A) were seen. Neither CA/AA genotypes nor A alleles were present in ALL patients and normal controls.
TC was the predominant haplotype in both groups, while CT proved to be minor. The cumulative incidence of relapse
was higher with the CC genotype of C1236T as compared with TT. Conclusion: From our preliminary data, the CT
genotype of C3435T is associated with a poor ALL outcome while the CC genotype of C1236T is related with an
increased incidence of relapse. Although our results provide assistance for oncologist choice of individual therapeutic
strategy taking the patient genetic repertoire into consideration, further investigations with larger sample size should
be conducted to validate our results.- انتشار مقاله: 12-10-1396
- نویسندگان: Roba M Talaat,Medhat Y K El-Kelliny,Basima A El-Akhras,Rania M Bakry,Khaled F Riad,Adel A Guirgis
- مشاهده