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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: male mice nutrition, Polyunsaturated fatty acids, Sex ratio, Sperm characteristics
- چکیده:
- چکیده انگلیسی: Several studies have indicated that feeding female animal with fatty acids could result in alteration of offspring sex ratio; however, there is little information available regarding the effect of feeding male animal with fatty acids on sex ratio of offspring. The aim of current study was to investigate the effect of paternal feeding with n-3 and n-6 polyunsaturated fatty acids (PUFAs) on sperm characteristics and sex ratio of offspring in mice. Male mice received 2.50% of palm oil (control), fish oil (n-3 PUFAs) or sunflower oil (n-6 PUFAs) for two months. Sperm kinematics and viability were assessed using computer-assisted sperm analyzer and eosin-Y staining, respectively. Additionally, female mice were randomly introduced to males of three experimental groups to determine reproductive parameters including litter size, conception rate and sex ratio. Fish oil enhanced sperm concentration, motility and viability, whereas sunflower oil decreased sperm concentration. Nevertheless, progressive motility, velocity, linearity and straightness of sperms were not affected by source of fatty acids. Although supplementation with fish oil resulted in male-biased sex ratio, palm and sunflower oils did not impact sex ratio. Besides, source of fatty acids failed to influence conception rate and litter size. In conclusion, the present study provided evidence for the impact of paternal fish oil consumption on sex ratio of offspring for the first time. Moreover, it showed beneficial effect of n-3 PUFAs and adverse effects of n-6 PUFAs on seminal parameters.
- انتشار مقاله: 26-05-1397
- نویسندگان: Beheshteh Abouhamzeh,Reza Youssefi,Vahid Akbarinejad,Ehsan Mirsadeghi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Testosterone,Extracellular matrix,Bovine testicular cells
- چکیده:
- چکیده انگلیسی: Testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. Given that extracellular matrix (ECM) facilitates differentiation of spermatogonial stem cells (SSCs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ECM on SSCs differentiation. In experiment 1, testosterone production was measured in testicular cells cultured for 12 days on ECM or plastic (control). In experiment 2, testosterone production was assessed in testicular cells cultured on ECM or plastic (control) and exposed to different concentrations of hCG. In experiment 3, the gene expression of factors involved in testosterone production was analyzed. Testosterone concentration was lower in ECM than in the control group in experiment 1 (p < 0.05). In experiment 2, testosterone concentration was increased in response to hCG in both groups but cells cultured on ECM were more responsive to hCG than those cultured on plastic (p < 0.05). In the experiment 3, qRT-PCR revealed the inhibitory effect of ECM on the gene expression of steroidogenic acute regulatory protein (StAR) (p < 0.05). Nevertheless, the expression of LH receptor was greater in ECM-exposed than in unexposed cells (p < 0.05). In conclusion, the present study showed that inhibiting the expression of StAR, ECM could lower testosterone production by Leydig cells during in vitro culture. In addition, the results indicated that ECM could augment the responsiveness of Leydig cells to hCG through stimulating the expression of LH receptor.
- انتشار مقاله: 24-11-1395
- نویسندگان: Vahid Akbarinejad,Parviz Tajik,Mansoureh Movahedin,Reza Youssefi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Mice,Androgen Receptor,sex ratio,Flutamide
- چکیده:
- چکیده انگلیسی: Maternal testosterone has been indicated to affect sex ratio of offspring. The present study was conducted to elucidate the role of androgen receptor in this regard by blockade of androgen receptor using flutamide in female mice. Mice were randomly assigned to two experimental groups. Mice in the control (n = 20) and treatment (n = 20) groups received 8 IU equine chorionic gonadotropin (eCG) followed by human chorionic gonadotropin (hCG) injection (8 IU) 47 hr later. In addition, mice in the control and treatment groups received four injections of ethanol-saline vehicle and flutamide solution (2.50 mg), respectively, started from 1 hr before eCG injection until hCG injection at 12-hr intervals. Conception rate was not different between the treatment (18/20: 90.00%) and control (19/20: 95.00%) groups (p > 0.05). Litter size was higher in the treatment (8.22 ± 0.26) than control (7.21 ± 0.28) group (p < 0.05). Male sex ratio was lower in the flutamide-treated mice (67/148: 45.30%) as compared with the untreated ones (80/137: 58.40%; odds ratio = 1.69; p < 0.05). In conclusion, the results showed that androgen receptor blockade could skew sex ratio of offspring toward females implying that the effect of testosterone on sex ratio might be through binding to androgen receptor. In addition, the blockade of androgen receptor using flutamide appeared to enhance litter size.
- انتشار مقاله: 09-03-1395
- نویسندگان: Faramarz Gharagozlou,Reza Youssefi,Mehdi Vojgani,Vahid Akbarinejad,Ghazaleh Rafiee
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: bovine,Differentiation,Spermatogonial stem cells,Fibroblast growth factor receptors
- چکیده:
- چکیده انگلیسی: The receptors 1 and 2 of fibroblast growth factor (FGFR1 and FGFR2, respectively) have been observed in all types of testicular cells. Culture on extracellular matrix (ECM) has been observed to lead to initiation of differentiation in spermatogonial stem cells (SSCs). The present study was carried out to investigate whether FGFR1 and FGFR2 play a role in SSCs differentiation. Following isolation, bovine testicular cells were cultured on ECM-coated or uncoated (control) plates for 12 days. The gene expression of THY1, cKIT, FGFR1 and FGFR2 was evaluated using quantitative real-time polymerase chain reaction (PCR). Results related to the gene expression of markers of with undifferentiated (THY1) and differentiated (cKIT) spermatogonia implicated stimulation of self-renewal and differentiation in cells cultured on ECM-coated and uncoated plates, respectively (p < 0.05). Concomitantly, the expression of FGFR2 increased during culture in the ECM group (p < 0.05), whereas it did not change in the control group (p > 0.05). As a result, the gene expression of FGFR2 was greater in the ECM than control group (p < 0.05). Nevertheless, FGFR1 expression did not change during culture in the control and ECM groups (p > 0.05). In conclusion, the present study revealed the potential role of FGFR2 in differentiation of SSCs during culture on ECM.
- انتشار مقاله: 09-03-1395
- نویسندگان: Vahid Akbarinejad,Parviz Tajik,Mansoureh Movahedin,Reza Youssefi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Dog,n-3 Fatty Acids,Secondary sex ratio
- چکیده:
- چکیده انگلیسی: The present study was conducted to evaluate the effect of fish oil supplementation prior to mating on secondary sex ratio of pups (the proportion of males at birth) in bitches. Sixty five bitches (German Shepherd, n = 35; Husky, n = 30) were enrolled in the study. Bitches (140-150 days post-estrus) were given 2% per dry matter intake palm oil and fish oil in the control (n = 33) and treatment (n = 32) groups, respectively. To induce estrus, bitches were received equine chorionic gonadotropin (eCG) administration (50 IU kg-1) 30 days after nutritional supplementation followed by human chorionic gonadotropin (hCG) administration (500 IU per dog) seven days later. Bitches were introduced to dogs of the same breed after hCG administration. The weight of bitches was increased over time (p < 0.05), but their weight change was not different between two groups (p > 0.05). The mating rate, pregnancy rate and litter size were not influenced by treatment and breed. Secondary sex ratio was higher in the treatment (105/164; 64.00%) than in the control (68/147; 46.30%) group (p < 0.05; adjusted odds ratio = 2.068). Moreover, secondary sex ratio was higher in Husky bitches (88/141; 62.40%) compared to German Shepherd (85/170; 50.00%; p < 0.05; adjusted odds ratio = 1.661). In conclusion, the present study showed that inclusion of fish oil in the diet of bitches prior to mating could increase the proportion of male pups at birth. In addition, it appears that there might be variation among dog breeds with regard to the sex ratio of offspring.
- انتشار مقاله: 09-03-1395
- نویسندگان: Faramarz Gharagozlou,Reza Youssefi,Vahid Akbarinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Abomasum,Cervus elaphus,Spiculoptenagia asymmetrica
- چکیده:
- چکیده انگلیسی: Spiculopteragia asymmetrica is a gastrointestinal nematode frequently found in the abomasum of cervids. During December and February 2010, two red deer were died in Semeskandeh sanctuary in Mazandaran province. Moreover, five live deer from mentioned area were treated by Ivermectin and collected feces of these animals were used for assessment helminthes infection by parasitological methods. Several nematodes were recovered in abomasums and in fecal samples of treated animals. Number of worms recovered from abomasums of two dead animals were 275 (90 male and 185 female) from the first one and 327 (102 male and 225 female) from the second. Based on morphological characteristics nematodes were diagnosed as S. asymmetrica. This is the first report of existence of S. asymmetrica from cervids in Iran.
- انتشار مقاله: 28-11-1392
- نویسندگان: Mohammad Reza Youssefi,Seyed Hossein Hoseini,Iraj Mobedi,Seyed Mohammad Hosseini,Behrang Ekrami
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Cat,Follicular ovarian cyst,cystic ovarian disease
- چکیده: در این گزارش، تشخیص و درمان یک مورد کیست تخمدانی فولیکولی در یک گربه پرشین ماده 5 ساله شرح داده میشود. گربه مورد نظر در ملامسه ناحیه پشت کمر، موقعیت لوردوزیس را به خود گرفته و پاهای عقب را بالا و پایین حرکت میداد.بررسی سونوگرافی ناحیه شکم دو کیست در تخمدان چپ گربه نشان داد. ارزیابی استروژن سرم خون، غلظت بالای 17 بتا_استرادیول (pg/mL 105) را نشان داد. با این حال غلظت پروژسترون نرمال (ng/mL 3/0) بود. بر این اساس، گربه مبتلا به کیست فولیکولار فعال تشخیص داده شد. حیوان مذکور با گنادوتروپین جفتی انسان (hCG) به صورت داخل عضلانی تحت درمان قرار گرفت. 30 روز پس از تجویز hCG، یک تزریق گنادوتروپین جفتی اسب (eCG) (50 واحد بین المللی) به صورت داخل عضلانی انجام شد. جفتگیری طبیعی با یک گربه نر پرشین بارور انجام گرفت. در نتیجه، به نظر میرسد که درمان دارویی کیستهای فولیکولار فعال را میتوان به منظور حفظ باروری در گربه سانان به ک ار گرفت.
- چکیده انگلیسی: In the present report, diagnosis and treatment of a case with follicular ovarian cysts in a 5-year-old Persian queen cat is described. In response to palpation of spines, the queen cat presented herself in lordosis and danced up and down with her rear legs. Trans-abdominal ultrasonography examination showed 2 cysts in the left ovary of the queen. Serum estrogen assay indicated elevated level of 17 β-estradiol concentration (105 pg/ml). However, progesterone concentration was normal (0.3 ng/ml). Accordingly, the queen was diagnosed with functional follicular cysts. The queen was treated with an administration of hCG intra-muscularly. Thirty (30) days after the administration of hCG, an injection of equine chorionic gonadotropin (eCG) (50 IU) was given intra-muscularly. Natural mating was done with a fertile Persian tom cat. In conclusion, it seems that treatment of functional follicular cysts can be applied to preserve fertility in cats.
- انتشار مقاله: 22-10-1393
- نویسندگان: Reza Youssefi,Parviz Tajik,Vrya Tohidi,Vahid Akbarinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Growth performance,Broilers,Coccidiosis,Diclazuril,Clopidol
- چکیده: زمینه مطالعه: کوکسیدیوز به عنوان یک بیماری انگلی که بیشترین تأثیر اقتصادی را بر تولید طیور دارد، شناخته شده است. صنعت مدرن پرورش طیور به میزان زیادی وابسته به ترکیبات ضد کوکسیدیوز است. هدف: مطالعه حاضر جهت تعیین و مقایسه کارایی دو ضد کوکسیدیوز شیمیایی (کلوپیدول و دیکلازوریل) بر دفع اووسیست و همچنین اثرات احتمالی آنها بر عملکرد پرورشی و کارایی تغذیهای طیور گوشتی در کوکسیدیوز تجربی انجام شده است. روش کار: تعداد 120 قطعه جوجه یک روزه نژاد Ross 308 بطور تصادفی گروهبندی شدند: کنترل مثبت؛ آلوده به کوکسیدیوز بدون درمان، گروه کنترل منفی؛ بدون آلودگی و بدون درمان، گروه CLO؛ آلوده و درمان شده باکلوپیدول، گروه DIC؛ آلوده و درمان شده با دیکلازوریل. برای ایجاد کوکسیدیوز تمامی گروهها بجز کنترل منفی با اووسیست اسپوروله عفونی در انتهای هفته سوم تلقیح شدند. میزان دفع اووسیست با استفاده از لام مک مستر شمارش گردید. وزن بدن، وزنگیری و ضریب تبدیل غذایی هر هفته اندازهگیری و محاسبه گردید. نتایج: شمارش اووسیست نشان داد که دیکلازوریل در کاهش تعداد اووسیست کارآمدتر از کلوپیدول بود. میانگین وزن بدن در گروه کنترل مثبت از سایر گروهها پایینتر بود. ولی در انتهای مطالعه میانگین وزن در گروههای درمان شده با کلوپیدول و دیکلازوریل با گروه کنترل مثبت تفاوت معنیداری را نشان نداد. در مقایسه وزنگیری میان گروههای مختلف بجز در هفته چهارم و پنجم در سایر هفتهها تفاوت معنیداری مشاهده نشد. بر پایه نتایج بدست آمده از FCR اضافه نمودن ترکیبات آنتی کوکسیدیال اثر معنیداری بر بهبود آن نداشت. نتیجهگیرینهایی: گزارش شده است که برخی از ترکیبات ضدکوکسیدیوز منجر به کاهش مصرف خوراک و ضریب تبدیل غذایی میشوند. به نظر میرسد هیچکدام از ضدکوکسیدیوزهای شیمیایی مورد استفاده در مطالعه حاضر در کنترل زیانهای اقتصادی کوکسیدیوز به طور کامل مؤثر نمیباشند و ارائه استراتژیهای جدید مورد نیاز میباشد.
- چکیده انگلیسی: Background: Coccidiosis is recognized as the parasitic disease that has the greatest economic impact on poultry production. Modern poultry production is largely dependent upon anticoccidials. Objectives: The aim of the present study was to determine and compare the efficacy of two chemical anticoccidials (Clopidol and Diclazuril) on oocyst shedding and their probable effects on growth performance and feed efficiency of broilers in experimental coccidiosis. Methods: One hundred and twenty Ross 308 one day old broilers were randomly assigned into 4 groups: CON+, infected with Eimeria mixed oocysts but not medicated; CON-, not infected and not medicated; CLO, infected and medicated with Clopidol; DIC, infected and medicated with Diclazuril. Chickens in all groups except negative control were inoculated by mixed sporulated oocysts of pathogenic Eimeria at the end of the 3rd week of age. Oocyst shedding was counted using a Mc Master counting chamber. Body weights, body weight gains, and feed conversion ratio were determined on weekly basis. Results: Obtained results showed that Diclazuril was more effective in reducing oocyst shedding than Clopidol. As expected, mean body weight in the positive control group was lower than other groups. But surprisingly, no significant difference was found between Diclazuril or Clopidol treated birds and positive control group, at the end of the experiment. In comparison of weight gain in different treatment groups except for the 4 and 5th week, no significant difference was found in different groups. In the last week of the experiment, difference in weight gain between groups was not significant. According to feed conversion ratio (FCR) results, anticoccidial supplementation did not have any significant effect on improving FCR. ConclusionS: It is reported that some anticoccidials reduce feed consumption and feed conversion. It seems that neither of the present synthetic anticoccidials is completely satisfactory in controlling coccidiosis economic losses and new strategies are urgently required.
- انتشار مقاله: 20-12-1393
- نویسندگان: Hamed Asadi Iraee,Mohammad Asadi Iraee,Mohammad Reza Youssefi,Mohaddeseh Abouhosseini Tabari
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Pelargonium roseum,pigeon,Metronidazole,Avian trichomoniasis,Trichomonas gallinae
- چکیده:
- چکیده انگلیسی: Objective: Pelargonium roseum Willd.(Geraniaceae) is widely grown as an ornamental plant due to its strong pleasant rose-like odor. The present study evaluates the antitrichomonal effect of P. roseum essential oil (EO) against Trichomonas gallinae both in vitro and in vivo and compares it to that of metronidazole (MTZ) as a standard antitrichomonal drug.
Materials and Methods: In vitro assays were accomplished in multi-well plates containing MTZ and EO at final concentrations of 2.5, 5, 10, 20, 50, and 100 μg/mL. In vivo assay was carried out on 40 experimentally infected pigeons receiving MTZ and EO at doses of 25 and 50 mg/kg.
Results: The 24-hr MIC of MTZ was 10 µg/mL, while for EO it was 20 µg/mL. Treatment with MTZ 50 mg/kg after 4 days led to full recovery of infected pigeons however EO 50 mg/kg resulted in the same outcome after 5 days. No mortality or clinical side effects were seen in treated birds.
Conclusion: The present study introduced P. roseum EO as a potent natural antitrichomonal agent effective against T. gallinae. Bioactive components of P. roseum can be used as potential therapeutic compounds in development of novel antitrichomonal drugs.- انتشار مقاله: 25-09-1395
- نویسندگان: Mohaddeseh Abouhosseini Tabari,Mohammad Reza Youssefi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Leishmania infantum,Pelargonium roseum,Promastigote,Glucantime
- چکیده:
- چکیده انگلیسی: Background and aims: Visceral leishmaniasis so called Kala-azar is one of the important health care problems in the world. Some herbal drugs are noticed because of toxicity and drug resistance against pentavalent antimonials as used in treatment of Kala-azar. In this study, anti-leishmanial activity of Pelargonium roseum Essential oil on the in vitro growth of Leishmania infantum promastigotes in comparison to Glucantime was studied.
Methods: Different concentrations of Pelargonium roseum essential oil on culture of Leishmania infantum promastigote were surveyed with Anti-leishmania activity assays at 24 hr and 48 hr at 570 nm wavelength. All the data were analyzed by using SPSS version and anti-leishmanial potency of the oil extract of Geranium in different concentration were compared with standard dose of Glucantime.
Results: All concentration in 24 hr showed no remarkable difference. 25, 50 and 100 µL/ml efficacy was same as Glucantime on the growth of promastigotes in 24 hr MTT. All concentrations except 1µL/ml in 48hr MTT had resemble impact on the growth of promastigotes. 5 µL/ml concentration of Pelargonium roseum essential oil was significant difference effect on the parasite (P= 0.043) in 48hr.
Conclusion: Pelargonium roseum essential oil doesn’t treat Leishmania infantum promastigote quickly. After 48 hr, 5 µL/ml concentration can be a suitable candidate in clinical trials.- انتشار مقاله: 04-07-1395
- نویسندگان: Mohaddeseh Abouhosseini Tabari,Elham Moghaddas,Bibi Razieh Hosseini Farash,Mohammad Amin Ebrahimi,Nilofar Nabavi Mousavi,Mohammad Reza Youssefi
- مشاهده