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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Personalized Medicine,Glioblastomamultiform,In vitro chemosensitivity and resistance assay,Primary,explant technique,Stereotactic biopsy
- چکیده:
- چکیده انگلیسی: Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs.
Materials and Methods: Single cell suspension was prepared from 1 g weight explants of the established xenograft tumor of GBM. Also, primary culture was carried out on 35 mg weight specimens, as a surrogate for stereotactic biopsies. Then, chemoresponse profile of cells obtained by direct cell disaggregation and primary culture was determined using temozolomide and carmustine by clonogenic assay[AGA1] .
Results: There was no statistically significant difference in the cytotoxicity of temozolomide and carmustine between cells obtained from both methods.
Conclusion: This work supports the feasibility of using stereotactic biopsies of GBM in CSRAs.- انتشار مقاله: 20-09-1393
- نویسندگان: Fariba Sadeghi Fazel,Mahnaz Haddadi,Alireza Khoshnevisan,Samad Muhammadnejad,Ahad Muhammadnejad,Zohreh Mazaheri,Motahareh Arjomandnejad,Reza Shirkoohi,Mohammad-Ali Oghabian,Narjes Sherkat-Khameneh,Saeid Amanpour,Monireh Kazemimanesh
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Biomarker,Metastasis,Molecular Genetics,Personalized Medicine
- چکیده:
- چکیده انگلیسی: It can be considered that cancer is a disease the prevalence of which has been increased with the world modernization. Many other diseases such as huge infectious pandemics and natural or unnatural disasters have already shorten the mean age of human beings and there has been little chance for neoplastic malignancies to come on sight among the population. Malignancies have various profiling based on the characteristics of the cell origin and tumor type. There are different alterations in genetic, epigenetic, protein function, microenvironment and other systematic interactions during disease progression. In some cases, using different types of drugs which are more compatible with the morphology of the majority of cells in a tumor can cause the other cells with different genetic and epigenetic profiling to proliferate which leads to treatment failure and progression of disease to more advance stages such as systemic metastasis. Personalizing therapeutic decision for advanced patients has been performed in recent years. There are different reports of improvement of quality of life or survival enhancement and even complete remission in some advanced cases as a result of managing tumor molecular information and cell signaling analysis. Personalized medicine is still in the beginning of its way and needs more investigation and infrastructural research. Finding new biomarkers and targets in cell signaling which is involved in different aspects of disease will be helpful to increase the percentage of sensitivity in decision making.
- انتشار مقاله: 19-12-1396
- نویسندگان: Reza Shirkoohi,Reza Shirkoohi,Reza Shirkoohi
- مشاهده