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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: mesenchymal stem cell,Cell Proliferation,Scaffold,Nano- and micro-hydroxyapatite,poly (llactic acid),Bone differentiation
- چکیده:
- چکیده انگلیسی: Bones constructed by tissue engineering are being considered as valuable materials to be used for regeneration of large defects in natural bone. In an attempt to prepare a new bone construct, in this study, proliferation and bone differentiation of marrow-derived mesenchymal stem cells (MSCs) on our recently developed composite scaffolds of nano-, micro-hydroxyapatite/ poly(l-lactic acid) were compared with pure
poly(l-lactic acid) scaffolds. For this purpose, some passaged-3 rat MSCs were three-dimensionally cultivated
on the scaffold surfaces and their morphology was observed with scanning electron microscopy. Cell
proliferations on different scaffolds were examined by MTT assays. Osteogenic cultures were established
with the scaffolds loaded with MSCs for 21 days at the end of which culture mineralization; the cell alkaline
phosphatase (ALP) Level and the relative expression of selected bone specific genes were quantified and
compared to each other. Our results indicated that the cells having been adhered and assumed spherical
morphology were able to proliferate in all studied scaffolds. The microenvironment provided by nano-scaffolds
appeared much better medium than those of micro-scaffolds and pure PLLA (P < 0.05). The osteogenesis
assays indicated to the superiority of nanoscaffolds in supporting MSCs undergoing bone differentiation,
which was reflected in high cellular ALP levels, increased bone-related gene expression and enhanced culture mineralization. Collectively, the bone construct prepared with nano-hydroxyapatite/ poly (llactic acid) scaffold and proliferated MSCs would be suitable candidate for use in bone regenerative medicine.
- انتشار مقاله: 09-07-1389
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Fatemeh Bagheri,Mojgan Zandi,Elham Nejati,Elham Zomorodian,Houri Mivehchi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR-RFLP,Genotypes,Alpha 1-antitrypsin deficiency,Phenotypes,IEF
- چکیده:
- چکیده انگلیسی: Alpha 1-antitrypsin (AAT) or alpha 1-protease inhibitor (PI) is the principal inhibitor of proteolytic enzyme in serum. Its phenotypic variability has been reported to be associated with liver, lung diseases and rheumatoid arthritis in humans. There is much documentation about high risk phenotypes of PI in some regions of the world, however, there are no reliable reports on these phenotypes and genotypes and their related diseases in Iranian population. The aim of this study was to determine PI phenotypes and genotypes in Iranian patients suffering from PI deficiency. For this purpose, whole blood samples from 307 patients suspected of diseases
related to PI deficiency, and 156 healthy persons were examined. PI phenotypes and genotypes were determined by isoelectric focusing (IEF) and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP), respectively. Allele frequencies from patients and normal subjects were compared. For reliability, a family study of the patients was also carried out. The PI phenotype frequencies of all six possible combinations of M, S and Z haplotypes in patients were: MM, 77.20%; MS, 6.18%; MZ, 7.17%; SS,
3.91%; ZZ, 4.56%; SZ, 0.98% and in normal subjects were: MM, 78.20%; MS, 5.76%; MZ, 15.38%; SS, 0.64%; 0% for ZZ and SZ. Analysis of data showed that there was a significant difference between patients (with liver, lung diseases and rheumatoid arthritis) and control subjects (p< 0.05). In Conclusion, the allelic frequencies of S and Z in the patient group were 7.49% and 8.63%, while in the normal subjects
were 5.13% and 4.17%, respectively. This is the first report of the prevalence of high risk alleles (Z and S) in patients suspected of PI deficiency and related diseases in Iran.- انتشار مقاله: 09-07-1384
- نویسندگان: Abbas S. Lotfi,Seyed Alireza Mesbah-Namin,Bahman GH Goudarzi,Abbas Zare Mirakabadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Nested-PCR,Amelogenin,Ancient DNA,Restriction enzyme digestion,Sex determination and Molecular archaeology
- چکیده:
- چکیده انگلیسی: Extraction and analysis of DNA from ancient remains has numerous applications in archeology and molecular
evolution. However, it has become obvious that ancient DNA (aDNA) can be easily contaminated with
modern DNA, so it is crucial to detect contamination and to distinguish contaminant from authentic results.
In the present study, we report the successful extraction and amplification of aDNA from 3000-3500 yearold
human remains excavated from Masjede kabood (Tabriz, North-West of Iran) burial site. To test the
authenticity of the extracted aDNA, we have developed a nested PCR/restriction enzyme digestion
method for molecular sex determination of the skeletal remains, which their gender was known based on their
morphology and belongings (Crown, Sword, Bracelet etc.). A simple and effective modified ethanol precipitation-based protocol was used for DNA extraction from 35 human skeletal remains. A segment of Homologous Amelogenin Gene (AMG), which has different alleles on X and Y-chromosomes, was amplified and analyzed. The obtained data were compared with anthropometric reports as a control for the rate of precision in aDNA analysis. The results showed that reliable aDNA can be extracted and amplified from archeological
remains. The presented sex determination procedure could also be used as a reliable control for testing the
authenticity of aDNA results.- انتشار مقاله: 10-07-1383
- نویسندگان: Elmira Mohandesan,Seyed Javad Mowla,Alireza Hojabri Noobari,Mohammad Mehdi Yaghoobi,Seyed Alireza Mesbah-Namin
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Behavioral Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Validation,Keywords: Translation,Family Resilience
- چکیده:
- چکیده انگلیسی: Introduction: Resilience is the process by which families are able to maintain or regain positive outcomes despite stress and negative emotional experiences. Assessing family resilience is important to identify families’ strengths and vulnerabilities. There are currently no family resilience instruments available for Iranian families. This paper describes the translation and validation of Walsh Family Resilience Questionnaire (WFRQ) for being used with Iranian families.
Method: The WFRQ was translated into Persian and back-translated. Proportional simple random sampling was used to recruit a representative sample of 350 families selected from a military center in 2017 in Tehran. Total scale and subscale reliability were examined using Cronbach alpha. Test- retest reliability was conducted with 350 adolescents who completed the WFRQ twice with a two-week interval in between and assessed using Intra-class Correlation Coefficients (ICC).
Results: Cronbach alpha coefficients were equal to or greater than 0.70 for all scales. Test-retest reliability for the overall WFRQ score was excellent (ICC = 0.83, 95%CI, 0.76–0.93) and very good for family belief system subscales (ICC = 0.70–0.79).
Conclusion: The present study provides evidence of good validity, reliability and test-retest reliability of the WFRQ for Iranian families. Further testing, including construct validity, and testing across different contexts will strengthen the evidence. The instrument will have applications in resilience research, educational and clinic settings and could facilitate the development and evaluation of intervention programs to build resilience in Iranian families.- انتشار مقاله: 06-04-1397
- نویسندگان: Mehdi Dadashi Haji,Reza Karaminia,Seyed Hossein Salimi,Mohsen Ahmadi Tahour
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Community Based Nursing and Midwifery
- نوع مقاله: Journal Article
- کلمات کلیدی: Education,Diabetes Mellitus,Peer group,Hemoglobin A,Glycosylated
- چکیده:
- چکیده انگلیسی: Background: Diabetes Education by Peer Coaching is a strategy which helps the patients with diabetes in the field of behavioral and emotional problems. However, the results of studies in this field in other countries could not be generalized in our context. So, the current study aimed to examine the effectiveness of Diabetes Education by Peer Coaching on Diabetes Management. Methods: Outcome variables for patients and peer coaches are measured at baseline and in3,6 and 12 months. The primary outcome consisted of Fasting Blood Sugar (FBS) and HbA1c. Secondary outcomes included Blood Pressure (BP), Body Mass Index (BMI,) Waist–Hip Ratio (WHR), Lipid Profile, diabetes self-care activities, diabetes-related quality of life, depression, and Social Capital levels.Initial analyses compared the frequency of baseline levels of outcome and other variables using a simple Chi-square test, t-test and the Mann-Whitney- U test. Sequential measurements in each group were evaluated by two-way analysis of variance. If significant differences in baseline characteristics were found, analyses were repeated adjusting for these differences using ANOVA and logistic regression for multivariate analyses. Additional analyses were conducted to look for the evidence of effect modification by pre-specified subgroups.Conclusion: The fact is that self-control and self-efficacy in diabetes management and treatment of diabetes could be important components. It seems that this research in this special setting with cultural differences would provide more evidence about peer-coaching model. It seems that if the peer-coaching model improves learning situations between patients with diabetes by offering one-on-one Diabetes Self Management Education, it could be an interactive approach to diabetic education.Trial Registration Number: IRCT201501128175N3
- انتشار مقاله: 24-12-1394
- نویسندگان: Maryam Aalaa,Mahnaz Sanjari,Hamid Reza Aghaei Meybodi,Mohammad Reza Amini,Mostafa Ghorbani,Hossien Adibi,Neda Mehrdad
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Mining and Geo-Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Sedimentary model,intrinsic compression,fabric,overconsolidation,Kerman City
- چکیده:
- چکیده انگلیسی: In this research, the effects of a sedimentary basin, environmental conditions, and the passage of time were investigated on consolidation processes and engineering characteristics of fine-grained soils in Kerman city. For this purpose, the natural consolidation curves of soil samples extracted from different locations of Kerman city were compared with the Kerman city intrinsic consolidation line (ICL). To obtain the ICL, 25 soil samples were taken from several locations and depths of the study area and mixed with high water content equivalent 1.0 to 1.5 times their liquid limit. Then, one-dimensional consolidation tests were performed on reconstituted soils. Using the normalizing parameter (void index) proposed by Burland and the ICL of Kerman city deposits, the results of consolidation tests were plotted on Iv- Log(σ́v) space. To investigate the effects of the sedimentary basin on engineering properties of fine-grained deposits in Kerman city, natural sedimentation compression curves were compared with ICL of Kerman soils. This comparison was applied in three regions along the paths of rivers that supply the fine sediments of Kerman plain. The results have shown that soil structure and fabric have not been developed abundantly and the effect of sedimentary model has caused the compression.
- انتشار مقاله: 31-01-1396
- نویسندگان: MohammadReza Aminizadeh,Iman Aghamolaie,Gholam Reza Lashkaripour,Mohammad Ghafoori,Naser Hafezi Moghadas
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Real-time PCR,Aqueous extract,Hexane extract,HSV-1,Chelidonium majus
- چکیده:
- چکیده انگلیسی: Herpes simplex virus type 1 (HSV-1) leads to various clinical manifestations in humans. Due to drug resistance, the use of herbs extracts for treatment has been considered as a significant solution. One of these compounds is Chelidonium majus L. herb extracts which is known as Mamiran in Iran. In this study, two aqueous and hexane extracts of Chelidonium majus L. were used to investigate the antiviral effects against HSV-1. The toxicity of hexane and aqueous extracts on HeLa cells was determined by MTT assay. Anti-viral effects of the extracts were evaluated directly, at different concentrations and times. The effect of the aforementioned extracts on reducing the virus replication was evaluated by quantitative Real-time PCR based on the glycoprotein D (gD) gene expression level of the herpes simplex virus. We observed that a 3500 μg/ml concentration of aqueous extract of C.majus and 600 μg/ml concentration of hexane extract were not toxic to HeLa cells. None of the extracts had a direct anti-viral effect on the virus. The aqueous extract at the above concentrations after 1, 2, 4, and 8 h of adsorption significantly reduced on virus replication. In contrast, the hexanic extract of this plant had no viral and inhibitory effect on HSV-1 replication. The aqueous extract of C.majus has an antiviral effect on HSV-1 compared to the hexane extract.
- انتشار مقاله: 24-06-1397
- نویسندگان: Shiva Mohammad,Gholamreza Amin,Mohammad Hassan Pouriayevali,Masoud Parsania
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR,Enterococcus,VanB and VanA
- چکیده:
- چکیده انگلیسی: Enterococci, as the major cause of nosocomial infections, have been shown to have an increase in drug resistance during the past two decades. TheEntrococcalemergence of Vancomycin-resistant is very important nowadays. The purpose of this study was to determine the drug sensitivity of Enterococcus faecalis and Enterococcus faecium species against Vancomycin, Tetracycline, Gentamicin, Erythromycin and the presence of Van A / B genes in resistant Enterococci to Vancomycin. A total of 189 samples, including urine, blood, wound and stool were collected from patients referred to Masoud Medical laboratory in Tehran, Iran, during years 2011-2012. Strains of Enterococci were identified by standard methods and antibiotic susceptibility testing was performed by disk diffusion according to standard methods (CLSI). The test was carried out on the samples resistant to Vancomycin for determining MIC levels. The frequencies of VanA and VanB were studied by using of PCR. A total of 89 Enterococcus were isolated in this study. In this study, 89 Enterococcus were obtained, of which 8 were resistant to Vancomycin based on the disk diffusion method. All Vancomycin resistant isolates showed VanA gene, while none of them (0%) carried VanB gene. The results obtained by this study indicated that Vancomycin-resistant Enterococci isolates did not commonly carry the VanA gene. However, VanA gene was fairly frequent as it was detected in 8 strains, while VanB gene was found in none of the strains.
- انتشار مقاله: 24-08-1393
- نویسندگان: Mohammad Reza Khataminejad,Zahra Ghanizadeh Dizaj,Saeed Zaker Bostanabad
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: children,Iran,Arthritis,Juvenile idiopathic arthritis,Uveitis
- چکیده:
- چکیده انگلیسی: Introduction: The purpose of this study was to describe clinical features and serological findings of children with idiopathic arthritis in south-western Iran.
Methods: This descriptive study included 60 patients with juvenile idiopathic arthritis who were referred to a pediatric rheumatology clinic at a university hospital during 6-month period. Initial manifestations, first laboratory tests and clinical course of patients were reviewed.
Results: Sixty children (32 boys and 28 girls) with idiopathic arthritis ranged in age from 1.5 to 16 years. The mean age at the first presentation was 4.92 years (SD= 3.68). Oligoarthritis was the most common subtype in 27 (45%), followed by systemic- onset in 17 (28.3%) and polyarthritis in 16 (26.7%) of patients. The most commonly involved joints were knee 53(88.3%), ankle 28(46.6%) and wrist 27(45%). Uveitis was detected in two patients, and positivity for ANA titer was revealed in one patient.
Conclusions: In this study, the pattern of most clinical features in different subtypes of juvenile idiopathic arthritis resembles to other studies. Positive ANA was less; however, the low numbers of Iranian patients with uveitis was noteworthy.- انتشار مقاله: 02-06-1393
- نویسندگان: Soheila Alyasin,Mozhgan Moghtaderi,Mansour Rahimi,Reza Amin,Sara Kashef
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Désert,Underground Dam,Subsurface flow,Collecting well,Ravar city,Land farming
- چکیده:
- چکیده انگلیسی: Agricultural droughts are droughts that affect the crop production or the ecology of a region. Ravar city that is located in margin of desert and the north of Kerman province is one of these areas. Due to the existence of enough underground water that entrance to desert and high evaporation to improving agricultural and cultivar activities, it needed to study the construction of underground dam in Ravar area. Therefore in order to (sake) controlling the subsurface flow that entrance to desert in west of Ravar city that is arid for cultivar agriculture, a site in the margin of desert in space of ten kilometers in the north of Kerman province (Ravar city) has selected and the needed studies has done in several step. At first the physiographic, hydrology, climatology, social economics and engineering geology study has done and the result shows the need of supplementary study. In second the geophysics study has done for Assessment of alluvial thickness and bed rock depths. in the third step the geotechnics activities for Assessment of exact depth of bed rock ,ground water table and sampling for field and laboratory testing in order to ( sake) Assessment of physical and mechanical specifics. In fourth step the pumpage test has done Assessment of hydrodynamics coefficient such as (T and S). At finally the design and executive of subsurface dam with the modern methods has done and the result of monitoring shows than the dam has constructed very successfully with positive effects to ground water flows
- انتشار مقاله: 27-05-1393
- نویسندگان: Alireza Karimzadeh,Mohammad Reza Aminizadeh Bazanjani
- مشاهده