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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Genetics and Plant Breeding
- نوع مقاله: Journal Article
- کلمات کلیدی: Gene expression,Stevioside,elicitation,Rebaudioside A,Stevia rebausiana
- چکیده: گیاه استویا (S. rebaudiana)، استویول گلیکوزیدهای استویوزید و ربادیوزید A را تولید میکند که به عنوان شیرینکنندههای کمکالری ارزش زیادی دارند. هدف از این مطالعه، بررسی آثار تحریک توسط اسید سالیسیلیک و زمانهای نمونهبرداری در بهبود تولید استویوزید و ربادیوزید A و بیان ژنهای KA13H، UGT74G1 و UGT76G1 است. نتایج نشان میدهد که افزودن غلظتهای مختلف اسید سالیسیلیک، بسته به مقدار استفاده از آن، آثاری متفاوت بر تولید استویوزید و ربادیوزید A دارد. بین غلظتهای مختلف اسید سالیسیلیک، بیشترین مقدار استویوزید 33/38 میلیگرم در گرم وزن خشک و ربادیوزید A 93/2 میلیگرم در گرم وزن خشک بود که به ترتیب 96 ساعت پس از تحریک با 90 میلیگرم در لیتر و 48 ساعت پس از تحریک با 60 میلیگرم در لیتر اسید سالیسیلیک به دست آمد. تحریک با اسید سالیسیلیک باعث افزایش بیان ژنهای KA13H و UGT74G1 و کاهش بیان ژن UGT76G1 شد. آنالیز همبستگی نشان داد که بیان ژن KA13H همبستگی مثبت با تولید استویوزید دارد. بیان ژن UGT74G1 همبستگی مثبت با تولید استیوزید و همبستگی منفی با تولید ربادیوزید A داشت. بیان ژن UGT76G1 نیز با تولید ربادیوزید A همبستگی مثبت و با تولید استویوزید همبستگی منفی داشته است. آنالیز qRT-PCR نشان داد که با افزایش تولید استویوزید ناشی از تحریک اسید سالیسیلیک، بیان ژنهای KA13H و UGT74G1 افزایش و بیان ژن UGT76G1 کاهش مییابد.
- چکیده انگلیسی: S. rebaudiana produces steviol glycosides including stevioside and rebaudioside A that are valuable as low calorie sweeteners. The objective of this study was to investigate the effects of salicylic acid elicitation and sampling times on the improvment of stevioside and rebaudioside A production and KA13H, UGT74G1 and UGT76G1 genes expression. The results showed that the addition of different concentrations of salicylic acid had different effects on stevioside and rebaudioside A production in a dosage-dependent manner. Among different concentrations of salicylic acid the highest amount of stevioside was 38.33 mg/g DW and the highest amount of rebaudioside A was 2.93 mg/g DW, observed 96 h after elicitation with 90 mg/L and 48 h after elicitation with 60 mg/L salicylic acid, respectively. Elicitation with salicylic acid increased KA13H and UGT74G1 genes expressions and decreased UTG76G1 gene expression. The correlation analysis indicated that, KA13H gene expression had a positive correlation with stevioside production. The UGT74G1 gene expression had a positive correlation with stevioside production and a negative correlation with rebaudioside A production. The UGT76G1 gene expression had a positive correlation with rebaudioside A and negative correlation with stevioside production. The qRT-PCR analysis indicated that, by increasing stevioside production under salicylic acid elicitation, the KA13H and UGT74G1 genes expression increased and UGT76G1 gene expression decreased.
- انتشار مقاله: 21-08-1397
- نویسندگان: Sajad Tahmasi,Ghasemali Garoosi,Jafar Ahmadi,Reza Farjaminezhad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Genetics and Plant Breeding
- نوع مقاله: Journal Article
- کلمات کلیدی: cumin,SSR,Genetic variability,Iranian ecotypes
- چکیده: این تحقیق جهت ارزیابی 49 اکوتیپ زیره سبز از 9 استان ایران با استفاده از نشانگرهای SSR انجام شد. بیشترین و کمترین باند چند شکل با استفاده از ترکیبات پرایمری به ترتیب 89 درصد برای Elap1479 ،Elap040 و Elap1493 و همچنین 56 درصد برای Elap017 و Elap1340 تولید شد. حجم اطلاعات چند شکلی بر اساس داده های مولکولی 13 ترکیب پرایمری (37/0-18/0) در بین تمام اکوتیپ ها متغیر بود. بالاترین میزان چند شکلی به میزان 0/37 متعلق به ترکیبات پرایمری Elap017 و Elap134 وکمترین آن به میزان 0/18 متعلق به ترکیبات پرایمری Elap1479 ،Elap040 و Elap1493 بود. نتایج در خصوص جمعیت های سمنان و خراسان شمالی با 8 باند بیشترین اختلاف را نشان داد در حالی که اصفهان و کرمان با کمترین اختلاف در باند ها نزدیک ترین جمعیت های به یکدیگر بودند. جمعیت های زیره سبز در سطح تشابه 0/71 به سه گروه تقسیم شدند. گروه اول شامل سمنان و خراسان جنوبی، گروه دوم فارس، کرمان، خراسان شمالی، خراسان رضوی، اصفهان و گلستان و در نهایت یزد گروه سوم را تشکیل دادند. بر اساس نتایج حاصل از کلاسترها اکوتیپ های کرمان و خراسان شمالی به دلیل سابقهی ژنتیکی نزدیک ممکن است متعلق به اجداد مشترکی باشند. می توان با استفاده از نشانگرهای مولکولی این تحقیق اطلاعات جامعی برای نشان دار کردن صفات اقتصادی به دست آورد. با توجه به تنوع پذیری و پتانسیل بالای جمعیت های زیره سبز ایران، این اطلاعات می توانند منبع مهمی برای اصلاح این گیاه باشند.
- چکیده انگلیسی: In this study, Simple Sequence Repeat markers (SSR) were used to investigate the genetic variation between 49 cumin ecotypes collected from 9 different provinces of Iran. SSR primers Elap1479, Elap040 and Elap1493 showed the highest (89%), while Elap1340 and Elap017 showed the lowest (56%) number of polymorphic bands. Polymorphism information content (PIC) values varied between 0.18 - 0.37. The highest and the lowest PIC value were obtained by Elap017 and Elap1340 (0.37) and Elap040, Elap1493 and Elap1479 (0.18), respectively. Cumin populations of Semnan and Northern-Khorasan showed the highest difference, whereas Kerman and Esfahan populations exhibited the lowest difference. The obtained dendrogram classified all cumin population into three clusters at similarity level of 0.71; the first group was Semnan and Southern-Khorasan populations, the second includes Fars, Kerman, Northern-Khorasan, Khorasan-Razavi, Esfahan, Golestan and the third class consisted of Yazd populations. Based on these clusters, Kerman and Northern-Khorasan showed the closet genetic background and may belong to the same ancestor. The genetic markers used in this study can provide impetus information for tagging of economic traits. It can be concluded that the variability in the Iranian cumin populations have potential important source for cumin breeding objectives.
- انتشار مقاله: 01-11-1391
- نویسندگان: Alireza Bahraminejad,Ghasem Mohammadinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Expression,Gene delivery,Vector,Ex-vivo,Human SOD1,Rat BMSCs
- چکیده:
- چکیده انگلیسی: Objective(s): Human superoxide dismutase 1 (SOD1) is the cytosolic form of this enzyme it detoxifies superoxide anions and attenuates their toxicities and concomitant detrimental effects on the cells. It is believed that the amount of these enzymes present in the oxidative stress-induced diseases is crucial for preventing disease progression. Transfection of rat bone marrow stromal cells (BMSCs) by a constructed vector carrying the human wild-type SOD1 gene, a non-viral gene transfer method, was the main aim of this study.
Materials and Methods: For this purpose, the rat BMSCs were transfected with the vector using Turbofect reagent and then stabilized. Western-blot and real-time PCR were also used for evaluation of SOD1 expression.
Results: Data analysis from RT-PCR and Western-blot techniques revealed that the stable transfected cells could secrete human wild-type SOD1 in the supernatant. Also, the total activity of SOD1 was about 0.5±0.09 U/ml and 0.005±0.002 U/ml in the supernatants of the transfected and not-transfected of rat BMSCs, respectively.
Conclusion: This study showed that expansion of the stable transfected rat BMSCs by a constructed vector carrying the human wild-type SOD1 gene is capable of secreting the active SOD1 enzyme under ex-vivo conditions. The recommendation of this study is that the same experiment would be applicable for expression of the other form of this enzyme, SOD3, as well. More valuable information could probably be provided about the variety of the diseases caused by superoxide anions toxicities by intervention and application of the non-viral method for expressions of SOD1 and SOD3 enzymes.- انتشار مقاله: 21-09-1396
- نویسندگان: Mohsen Abedi,Seyed Alireza Mesbah-Namin,Ali Noori-Zadeh,Taki Tiraihi,Taher Taheri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: eugenol,Stem cells,aging,Telomere,Telomerase,Cinnamaldehyde
- چکیده:
- چکیده انگلیسی: Objective(s): To investigate the effect of cinnamaldehyde and eugenol on the telomere-dependent senescence of stem cells. In addition, to search the probable targets of mentioned phytochemicals between human telomere interacting proteins (TIPs) using in silico studies.
Materials and Methods: Human adipose derived stem cells (hASCs) were studied under treatments with 2.5 µM/ml cinnamaldehyde, 0.1 µg/ml eugenol, 0.01% DMSO or any additive. The expression of TERT, AKT1 and DKC1 genes and the telomere length were assessed over 48-hr treatment. In addition, docking study was conducted to show probable ways through which phytochemicals interact with TIPs.
Results: Treated and untreated hASCs had undetectable TERT expression, but they did affect the AKT1 and DKC1 expression levels (CI=0.95; P<0.05). The telomere lengths reduced in phytochemicals treated with hASCs when compared with the untreated cells (P<0.05). Docking results showed that the TIPs might be the proper targets for cinnamaldehyde and eugenol. Data mining showed there are many targets for cinnamaldehyde and eugenol in the intracellular environment.
Conclusion: The general effect of cinnamaldehyde and eugenol is their induction of stem cell senescence. Therefore, they could be applicable as chemo-preventive or antineoplastic agents.- انتشار مقاله: 11-12-1395
- نویسندگان: Abdorrahim Absalan,Seyed Alireza Mesbah-Namin,Taki Tiraihi,Taher Taheri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Carbopol 934,Mucoadhesive discs,Pharmaceutical tests,PVP(Polyvinylpyrrolidone),Ziziphus jujuba stem bark
- چکیده:
- چکیده انگلیسی: Objective (s): Mucoadhesive disc is one of the various routes of drug delivery for curing buccal disease.Materials and Methods:Everydiscs containing 70 mg stem bark extract of Ziziphus jujuba were formulated by using Carbopol 934, PVP k30 and gelatin as polymers. Discs were made by granulation and direct compression. Discs were standardized based on the total phenol. Properties such as in vitro and in vivo mucoadhesion, drug release, water uptake, and disintegration were carried out. Results: Discs showed excellent mucoadhesion and released high amount of the active ingredients (47%) immediately and completed after approximately the first hour. They had a good adhesion in buccal cavity. Conclusion:This study showed that the kinetics of release of the active substance from the mucoadhesive disc obeyed the zero order kinetic and didn’t follow the fick’s law. The water uptake and dissolution (DS), increased with the passing of time.
- انتشار مقاله: 27-12-1394
- نویسندگان: Shokouhsadat Hamedi,Mohammad Reza Shams-Ardakani,Omid Sadeghpour,Gholamreza Amin,Dawood Hajighasemali,Hossein Orafai
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Systematic review,Methicillin-resistant staphylococcus aureus
- چکیده:
- چکیده انگلیسی: Objective(s)
Staphylococcus aureus is a prevalent pathogen worldwide. Methicillin resistant S. aureus (MRSA), which is usually multi-resistant in hospitals, has been a daunting challenge for clinicians for more than half a century. The aim of this systematic review and meta-analysis is to determine the relative frequency (RF) of MRSA in different regions of Iran.
Materials and Methods
Search terms “Staphylococcus aureus”, “Methicillin”, “mecA” and “Iran” were used in PubMed, Scirus and Google Scholar. Two Persian scientific search engines and ten recent national congresses were also explored. Articles/abstracts, which used clinical specimens and had done PCR to detect the mecA gene, were included in this review. Comprehensive Meta-Analysis and Meta-Analyst software were used for statistical analysis.
Results
Out of 2690 results found in the mentioned databases, 48 articles were included in the final analysis. These studies were done in Ahvaz, Falavarjan, Fasa, Gorgan, Hamedan, Isfahan, Kashan, Mashhad, Sanandaj, Shahrekord, Shiraz, Tabriz, Tehran and Tonekabon. Pooled estimation of 7464 S. aureus samples showed that 52.7%±4.7 (95% confidence interval [CI]) of strains were mecA positive. MRSA RF in different studies varied from 20.48% to 90% in Isfahan and Tehran, respectively. We found a moderate heterogeneity (I2= 48.5%) of MRSA RF among studies conducted in Tehran (ranging from 28.88% to 90%, mean 52.7% [95% CI: 46.6%±58.8%]).
Conclusion
According to the results of this study, MRSA RF in Iran is in the high range. Thus, measures should be taken to keep the emergence and transmission of these strains to a minimum.- انتشار مقاله: 26-06-1394
- نویسندگان: Emran Askari,Fatemeh Soleymani,Arash Arianpoor,Seyed Meghdad Tabatabai,Aminreza Amini,Mahboobeh Naderi Nasab
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: mesenchymal stem cell,Cell Proliferation,Adipose tissue,Cell aging,Cell differentiation
- چکیده:
- چکیده انگلیسی: Objective(s)
Some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. In the present study proliferation capacity and aging of such cells were explored.
Materials and Methods
Adherent cells were isolated from the collagenase digests of adipose tissues excised from rat epicardial and epididymal regions and propagated with several subcultures. The cells were then investigated whether or not they were able to differentiate into bone, cartilage and adipose cell lineages. Studied cells from two adipose tissues were also compared with respect to their in vitro proliferation capacity. The presence of senescent cells in the culture was determined and compared using senescence-associated (SA) ß-galactosidase staining method.
Results
Successful differentiations of the cells were indicative of their mesenchymal stem cells (MSCs) identity. Epicardial adipose-derived cells tended to have a short population doubling time (45±9.6 hr) than the epididymal adipose-derived stem cells (69±16 hr, P< 0.05). Colonogenic activity and the growth curve characteristics were all better in the culture of stem cells derived from epicardial compared to epididymal adipose tissue. Comparatively more percentage of senescent cells was present at the cultures derived from epididymal adipose tissue (P< 0.05).
Conclusion
Our data emphasize on the differences existed between the stem cells derived from adipose depots of different anatomical sites in terms of their proliferative capacity and in vitro aging. Such data can help understand varying results reported by different laboratories involved in adipose stem cell investigations.- انتشار مقاله: 29-06-1394
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Soura Mardpour,Marzieh Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Osteogenesis,Adipogenesis,Chondrogenesis,Bovine serum,Cell seeding density,Goat mesenchymal stem cells
- چکیده:
- چکیده انگلیسی: Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then differentiated among the osteogenic, adipogenic and chondrogenic cell lineages to determine their MSC nature. Differentiations were determined by RT-PCR analysis of related gene expression. To identify the best culture conditions for propagation, passage-3 cells were plated either at varying cell densities or different fetal bovine serum (FBS) concentrations for a week, at the end of which the cultures were statistically compared with respect to the cell proliferation. In this study, we also determined goat MSC population doubling time (PDT) as the index of their in vitro expansion rate. Results Passage-3 fibroblastic cells tended to differentiate into skeletal cell lineages. This was evident in both specific staining as well as the specific gene expression profile. Moreover, there appeared to be more expansion when the cultures were initiated at 100 cells/cm[1] in a medium supplemented with 15% FBS. A relatively short PDT (24.94±2.67 hr) was a reflection of the goat MSC rapid rate of expansion. Conclusion Taken together, fibroblastic cells developed at goat marrow cell culture are able to differentiate into skeletal cell lineages. They undergo extensive proliferation when being plated at low cell density in 15% FBS concentration.
- انتشار مقاله: 08-07-1394
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Hamid Nazarian,Fahimeh Falahi,Leila Taghiyar,Mohamad Taghi Daneshzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Mesenchymal Stem Cells,Osteogenesis,Lithium chloride
- چکیده:
- چکیده انگلیسی: Objective(s)
It is believed that the mesenchymal stem cell (MSC) differentiation and proliferation are the results of activation of wnt signaling pathway. On the other hand, lithium chloride is reported to be able to activate this pathway. The objective of this study was to investigate the effect of lithium on in vitro proliferation and bone differentiation of marrow-derived MSC.
Materials and Methods
In this experimental study, rat marrow cells were plated in a medium supplemented either with or without 2-10 mM lithium and expanded through three successive subcultures. To explore the impact of lithium on cell growth, doubling time (DT) of marrow cell population was determined for all the cultures. To determine the lithium effects on osteogenesis, the proliferation medium of passged-3 cells from all cultures were replaced by osteogenic media, with or without 2-12 mM lithium. Osteogenesis was then quantified by measurement of the amount of matrix mineralization and the expression of bone-specific genes.
Results
DT results indicated that the marrow cells in 4 mM lithium concentration were grown faster than the others (P<0.05). Intensive matrix mineralization and abundance of bone specific gene expression were observed in the cultures with 10-12 mM lithium concentration. All these differences were statistically significant. According to the results, all lithium -treated cultures possessed more differentiation than the control. Moreover, lithium low concentration was associated with more proliferation and its high concentration with more differentiating effects.
Conclusion
Lithium chloride at 4 mM concentration promotes MSC proliferation and at 10-12 mM enhances MSC osteogenic differentiation.- انتشار مقاله: 14-07-1394
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Mahmood Talkhabi,Bahman Zeynali
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Mesenchymal Stem Cells,Articular cartilage,Bone and fat differentiation,cartilage
- چکیده:
- چکیده انگلیسی: Objective Articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. On the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. Little is known about the presence of mesenchymal stem cells in articular cartilage tissue. This subject is investigated in the present study. Materials and Methods Articular cartilage was obtained from rats of Wistar strain and subjected to enzymatic digestion using 1:1 ratio of 0.2% collagenase I and 0.1% pronase. The released cells were then collected and cultivated in 25 cm2 culture flask, which was resulted in the appearance of two morphologically distinct cell populations. Fibroblastic population were purified and expanded through several subcultures, using their loose adherence on culture surfaces and examined in terms of differentiation potential. Results The primary culture mainly contained polygonal cells with few fibroblastic cells among them. Upon the first subculture, the fibroblastic cells appeared as aggregates of few cells among the polygonal cartilage cells. It was found that the fibroblastic cell population could easily detach from the culture surface following to a brief exposure to tripsin solution. Using this property, these cells purified and expanded by several subcultures. According to the results, the isolated cells were able to easily differentiate into chondrocytic, osteocytic and adipocytic cell lineages. Conclusion It seems that articular cartilage contains a distinct fibroblastic cell population with mesenchymal stem cell nature. These cells can be purified and expanded by cell culture methods, thanks to their loose adherence on the culture dish surfaces.
- انتشار مقاله: 19-07-1394
- نویسندگان: Mohammad Reza Baghaban Eslaminejad,Leila Taghiyar
- مشاهده