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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Objectives- To assess the ectopic bone formation in canine masseter muscle following the implantation of the natural bovine bone minerals (NBM) loaded with canine mesenchymal stem cells (MSCs). Design- Experimental study. Animals- four mongrel dogs. Procedures- Tripotent MSCs isolated from the canine bone marrow were loaded onto the NBM sponges and allowed to adhere. The cell-loaded scaffolds were then implanted in parallel with cell-free control scaffolds in masseter muscles of four mongrel dogs. Eight weeks after, the animals were sacrificed and the ectopic bone formation in implantation site was studied using the sections prepared from the parts of the muscle containing the implants. Furthermore, the amount of bone formation in two studied groups was quantified using Image-Pro Plust software. Results- The implants from the both groups were appeared to be encapsulated by fibrous tissue in implantation site which included some trabecular bone containing osteocyte and osteoblast. There were no indications of inflammation and foreign body reaction, nor were there any indications of cartilage tissue formation. In contrast to control, in MSCs group, lamellar bone was observed in some area. More importantly, in cell loaded scaffolds more amount of bone was formed compared to that of control cell free scaffolds (P<0.05). Conclusion and Clinical Relevance- Taken together it seems that in vivo bone forming capacity of the NBM sponges would be improved by loading it with MSCs.
- انتشار مقاله: 14-05-1386
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Mohammad Jafarian,Arash Khojasteh,Fatemeh Mashhadi Abbas,Mohammad Mehdi Dehghan,Bahar Houshmand
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Ultrasonography,Collagenase,SDFT,tendinitis
- چکیده:
- چکیده انگلیسی: Objective- The aim of this study was to assess the clinical and ultrasonographic alterations in the superficial digital flexor tendon (SDFT) of horses with collagenase-induced tendinitis. Design- Experimental study. Animals- Five clinically normal adult horse. Procedures- Two thousand units of collagenase were injected in the center of the SDFT of forelimbs of each horse under ultrasound guidance. Clinical examinations were performed to evaluate heat, response to palpation, presence of swelling, and lameness grade. Ultrasound images of the SDFT were recorded prior to injury and at 1, 3, 5, 7, and 14 days after injection. Images were digitized, and percentage of lesion, the echogenicity score (ES), and the fiber alignment score of the fibers (FS) at the maximum injury zone (MIZ) were measured. The sum of the core lesion from 8–20 cm distal to the accessory carpal bone was also measured. Results- Collagenase resulted in anechoic core lesion involving 20%-35% of the tendon cross section at MIZ. There were not any significant difference in the size, ES, and FS between all the days except between the first day and the other days. There were no significant differences between observations made of each structure in the left and right forelimbs of the same horse. Conclusions and Clinical Relevance- Collagenase-induced injury is ultrasonographically similar to naturally occurring injury and causes the same tendinitis in both left and right forelimbs and also in different horses. Tendon lesions using collagenase injection represent an experimental model for tendon injury in research trials.
- انتشار مقاله: 28-05-1392
- نویسندگان: Mohammad Mehdi Dehghan,Hossein Kazemi Mehrjerdi,Majid Masoudifard,Mohammad Reza Baghban Eslaminejad,Davood Sharifi,Ali Reza Vajhi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Screening,Prostate cancer,Prostate-Specific Antigen,Neoplasm grading
- چکیده:
- چکیده انگلیسی: Background: Prostate cancer is the most common malignancy among the male population in the United States and the 3rd most common non-skin cancer among men in Iran. Its prevalence has shown a rising trend in recent decades. The aim of this study was to report the epidemiological features of prostate cancer in patients referred for prostate biopsy in the south of Iran and to evaluate the accuracy of the levels of the prostate-specific antigen (PSA) and the PSA-density (PSAD) as well as the extension of the disease in the prediction of the biological behavior of prostate cancer.Methods: This is a retrospective study on the medical records of 1982 consecutive patients who underwent transrectal ultrasound-guided biopsy due to an abnormal digital rectal examination and/or an elevated PSA level following referral from the Urology Ward to the Radiology Department of Shahid Faghihi Hospital in Shiraz, southern Iran, between December 2003 and July 2014.Results: The overall cancer detection rate was 33.1%. Although the cancer was more prevalent among the elderly patients, a significant fraction (7%) of the patients were aged < 55 years. The sensitivity and specificity of the PSA were 97.4% and 8.7% and those of the PSAD were 82.9% and 52%, respectively. Of the 637 patients with prostate cancer, 250 (39.2%) had unilateral disease, 378 (59.4%) had bilateral disease, and 9 (1.4%) had inner-gland involvement. Most of the patients with bilateral involvement had high-grade Gleason scores.Conclusion: Our study underlines the relationship between age and the frequency of cancer; the levels of the PSA and the PSAD and the Gleason score; and the extent of tumor involvement and the grade of prostate cancer and also highlights the significance of screening, especially in younger patients.
- انتشار مقاله: 11-12-1395
- نویسندگان: Mehrzad Lotfi,Reza Beheshti,Mohammad Reza Rouhezamin,Abbas Rezaianzadeh,Pouya Farhadi,Zeinab Daneshi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Diet,lipid profile,Glucose,Body weight,Multiple birth offspring
- چکیده:
- چکیده انگلیسی: Background: Health status of offspring is programmed by maternal diet throughout gestation and lactation. The present study investigates the lasting effects of maternal supplementation with different amounts of soy oil or extra virgin olive oil (EVOO) on weight and biochemical parameters during gestation and lactation of female mice offspring.Methods: Eight weeks old female C57BL/6 mice (n=40) were assigned through simple randomization into four isocaloric dietary groups (16% of calories as soy oil (LSO) or EVOO (LOO) and 45% of calories as soy oil (HSO) or EVOO (HOO)) during three weeks of gestation and lactation. After weaning (at 3 weeks), all offspring received a diet containing 16% of calories as soy oil and were sacrificed at 6 weeks. Two-way ANOVA was used to adjust for confounding variables and repeated measures test for weight gain trend. Statistical analyses were performed with the IBM SPSS package.Results: At birth and adolescence, the weight of offspring was significantly higher in the soy oil than the olive oil groups (P<0.001 and P<0.001, respectively). Adolescence weight was significantly higher in the offspring born to mothers fed with 16% oil than those with 45% oil (P=0.001). Serum glucose, triglyceride and total cholesterol were significantly higher in the LSO than LOO (P<0.001, P<0.001 and P<0.001), LSO than HSO (P<0.001, P=0.03 and P<0.001), and LOO than HOO (P<0.001, P<0.001 and P<0.001) dietary groups, respectively. Serum triglyceride and total cholesterol were significantly higher in the offspring of HSO than HOO fed mothers (P<0.001 and P<0.001, respectively).Conclusion: A maternal diet containing EVOO has better effects on birth weight, as well as weight and serum biochemical parameters in offspring at adolescence.
- انتشار مقاله: 16-08-1394
- نویسندگان: Seyedeh Neda Mousavi,Fariba Koohdani,Farzad Shidfar,Mohamadreza Baghban Eslaminejad,Pantea Izadi,Mohamadreza Eshraghian,Leila Shafieineek,Hamidreza Tohidinik
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Mesenchymal Stem Cells,Mouse,6-bromoindirubin-3 -oxim
- چکیده:
- چکیده انگلیسی: Background: Hyaline cartilage defects exhibit a major challenge in the field of orthopedic surgery owing to its limited repair capacity. On the other hand, mesenchymal stem cells (MSCs) are regarded as potent cells with a property of cartilage regeneration. We aimed to optimize marrow-derived MSC chondrogenic culture using a small bioactive molecule referred to as BIO. Methods: MSCs from the marrow of NMRI mice were extracted, culture-expanded, and characterized. Micro-mass culture was then established for chondrogenic differentiation (control group). The cultures of MSC in chondrogenic medium supplemented with 0.01, 0.05, 0.1, and 1 µM BIO were taken as the experimental groups. Cartilage differentiation was examined by both histological sections and real-time PCR for Sox9, aggrecan, and collagen II at different time points. Moreover, the involvement of the Wnt pathway was investigated. Results: Based on histological sections, there was seemingly more intense metachromatic matrix produced in the cultures with 0.01 µM BIO. In this experimental group, cartilage-specific genes tended to be upregulated at day 14 compared to day 21 of the control group, indicating the accelerating effect of BIO on cartilage differentiation. Overall, there was statistically a significant increase (P=0.01) in the expression level of cartilage-specific genes in cultures with 0.01 µM BIO (enhancing effects). These upregulations appeared to be mediated through the Wnt pathway evident from the significant upregulation of T-cell factor and beta-catenin molecules (P=0.01). Conclusion: Taken together, BIO at 0.01 µM could accelerate and enhance in vitro chondrogenesis of mouse marrow-derived MSCs.
- انتشار مقاله: 10-12-1392
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Nasrin Fallah
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی:
- انتشار مقاله: 01-10-1392
- نویسندگان: Mohammad Mehdi Hosseini,Saeed Shakeri,Farhad Manaheji,Alireza Aminsharifi,Shahrokh Ezatzadegan,Maryam Pakfetrat,Mitra Basiratnia,Mahsa Hosseini
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: immunohistochemistry,Bladder tumor,Renal Stone
- چکیده:
- چکیده انگلیسی: Nephrogenic adenoma is a rare benign lesion of bladder that may be confused with malignant lesions. There is a strong relation with urinary tract irritation and intravesicle instrumentations. Nephrogenic adenoma was initially thought to originate from urothelial metaplasia; however, no solid proof is available. We present a case of 55-year-old lady with urinary problem. Cystocopic examination showed a sessile mass, and biopsy revealed circumscribed proliferation of tubules, cysts, and papillae that were lined by low cuboidal to columnar epithelial cells. Nephrogenic adenoma can be a significant diagnostic pitfall due to the presence of certain histological features such as the presence of enlarged nuclei with prominent nucleoli. Immunohistochemistery study was strongly positive for CK7, P504S, CD10, and EMA, but negative for CK20, PSA, and P63.
- انتشار مقاله: 11-09-1392
- نویسندگان: Mohamad Reza Farzaneh,Akbar Safaei,Ali Reza Amin Sharifi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Proliferation,Mesenchymal Stem Cells,Dental pulp,odontoblast
- چکیده:
- چکیده انگلیسی: Background: It is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (MSCs). The aim of the present study was to examine whether or not they are MSCs, or are distinct stem cells population residing in tooth pulp. Methods: Adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through several successive subcultures. Passaged-3 cells with a tendency to differentiate into odontoblastic cells were used to examine the key properties of MSCs including typical tripotent differentiation potential into bone, cartilage and adipose cell lineages and the expression of typical surface antigens. Moreover, they were examined for growth capacity in culture. Results: Dental pulp stem cells successfully progressed towards differentiation among three skeletal cell lineages. More than 90% of the cell population exhibited the expression of surface antigens known to be found on mesenchymal lineages such as CD105, CD90, CD44, and CD73, while only less than 2% expressed endothelial-hematopoietic epitopes including CD56, CD11b, CD34, CD31, CD33, and CD45. The cells exhibited a relatively high proliferation capacity with population doubling time of about 21.9 hours. Conclusion: The dental pulp stem cells are of MSC population, and may be considered suitable for use in regenerative medicine, owing to their relatively rapid rate of in vitro propagation.
- انتشار مقاله: 21-12-1393
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Hamid Nazarian,Mahsa Shariati,Sourena Vahabi,Fahimeh Falahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Estrogen might play an important role in the pathogenesis of diabetes mellitus type 2. Estrogens inhibit diabetes via distinct mechanisms particularly by reducing both hyperglycemia and plasma insulin levels. Estrogen exerts its physiological effects mainly through estrogen receptors including α and β types. Estrogen receptors are found in many tissues that participate in the pathogenesis of type 2 diabetes. Two common polymorphisms, PvuII and XbaI in estrogen receptor α gene, are reported to be associated with decreased receptor activity and increased risk of diabetes. We aimed to investigate the association between estrogen receptor α polymorphisms and diabetes, where a genetic component may be the major risk factor for this disease. One hundred women with diabetes type 2 were compared with one hundred women without diabetes for PvuII and XbaI polymorphisms. Of whom 61% of cases and 29% of controls had coronary artery disease. The participants were genotyped for these polymorphisms using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. The genotype distribution and frequency of mutated allele showed no significant differences between diabetic and non-diabetic groups in PvuII (χ2=0.981; P=0.612) and XbaI (χ2=0.362; P=0.83) polymorphisms. When coronary artery disease as the potential confounding factor was controlled by logistic regression analysis, it was found that the PvuII and XbaI variants were not related to the type 2 diabetes mellitus (P=0.60 and P=0.99, respectively). Neither PvuII nor XbaI genotypes was associated with increased susceptibility to the type 2 diabetes mellitus in selected Iranian women with diabetes and coronary artery disease.
- انتشار مقاله: 28-02-1394
- نویسندگان: Shekufeh Golkhu,Mahboobe Ghaed,Narges Mohammad Taghvaie,Mohammad Ali Boroumand,Gholamreza Davoodi,Alireza Aminzadegan,Leila Poorgoli,Mahmood Sheikh Fathollahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background: Cell-based treatment approach using differentiated mesenchymal stem cells (MSCs) and mature chondrocytes has been considered as an advanced treatment for cartilage repair. We investigated the differentiated level of these two cell types that is crucial for their repair capacity for cartilage defect at a co-culture micro mass system. Methods: Passaged-2 MSCs isolated from the mouse bone marrow and the primary-cultured chondrocytes obtained from rat costal cartilage were mixed at different ratios including 1:1, 1:2, and 2:1, and cultivated in the micro mass culture systems (experimental groups). Both the MSCs and chondrocytes alone in micro mass cultures were considered as the controls. After 21 days, the cultures were sectioned and examined by toluidine blue staining. Furthermore, the cells at different groups were analyzed by semiquantitative reverse transcription-polymerase chain reaction using the specific primers designed to detect the expression of both mouse and rat cartilage-specific genes. Results: According to the toluidine blue staining, metachromatic stain appeared to be more intense at 1:2 ratios than the other groups. Based on semiquantitative analysis, all co-cultures possessed significantly more cartilage-specific gene expression than the controls (P<0.01). While mouse aggrecan and collagen II genes had significantly more expression at 1:2 ratio, rat collagen II gene was expressed in higher rate at co-culture with 2:1 ratio (P<0.01). Conclusion: Co-culture of MSCs with mature chondrocytes seemed to provide an appropriate microenvironment whereby the two cell types exhibit higher differentiated phenotype than when they were cultured alone, and sufficient to be used as the cellular material for repair of cartilage defects.
- انتشار مقاله: 23-02-1394
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Leila Taghiyar,Fahimeh Falahi
- مشاهده