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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Esophageal Cancer,Haptoglobingenotype,Cancer risk factors
- چکیده:
- چکیده انگلیسی: Introduction: Esophageal cancer is one of the most lethal gastrointestinal cancers that has a complex and diverse etiology, with several genetic and nutritional factors involved in its etiology. The purpose of this study was to investigate the type of haptoglobin genotype and its relationship with some nutritional and biochemical risk factors affecting the prevalence of esophageal cancer in patients with early stage esophageal cancer. Materials and methods: In this study, 44 patients (20 males and 24 females) with early stage esophageal cancer and 44 healthy subjects, classified as control group, (19 males and 25 females) were selected. Haptoglobin (HP) genotype was determined employing PCR technique. Nutritional data were analyzed using standard food frequency questionnaire (FFQ) method. Serum levels of malondialdehyde (MDA), nitrate and nitrite were measured employing the colorimetric method. Serum levels of p53 protein were measured using the enzyme-linked immunosorbent assay (ELISA) technique. Results: The results of our study showed for the first time that HP1-1 genotype was the most prevalent genotype in esophageal cancer patients in Golestan province, Iran. HP2-2 genotype was the most frequent in the control group. Serum levels of MDA were significantly higher in the patients’ group compared to the control group (P˂0.001). Weight and body mass index (BMI) were significantly lower in the patients’ group than the control group (P<0.01). Food frequency analysis revealed that the consumption of fruits and vegetables in the patients’ group was lower than that of the control group (P<0.05). Conclusion: The results of our study showed for the first time that HP1-1 genotype is the dominant genotype in patients with esophageal cancer in Golestan province. As well, modification of nutritional pattern and consumption of high level of antioxidant compounds can be effective in reducing the prevalence of esophageal cancer in this region.
- انتشار مقاله: 06-07-1397
- نویسندگان: Sara Hosseinzadeh,Reza Alipanah-Moghadam,Fazel Isapanah Amlashi,Ali Nemati
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Glial cells,Tumor cell lysate,Dendritic Cells,MSC1-derived MVs,cancer immunotherapy
- چکیده:
- چکیده انگلیسی: Background: Immunotherapy is one promising therapeutic strategy against glioma, an aggressive form of brain
cancer. Previous studies have demonstrated that multiple tumor antigens exist and can be used to induce tumor specific
T cell responses. Furthermore, recently it was shown that TLR4-primed mesenchymal stem cells (MSCs), also known
as MSC1, mostly elaborate pro-inflammatory mediators. Compared to MSCs, MSC-derived microvesicles (MVs) have
advantageous properties that present them as stable, long lasting effectors with no risk of immune rejection. Therefore,
peripheral blood monocyte derived dendritic cells (MoDCs) have been used to load tumor antigens and stimulate T
cell mediated responses in the presence of MSC1-derived MVs in vitro. Methods: The B92 tumor cell line was heated
to 43°C for 90 min prior to preparation of tumor cell lysates. MVs were purified by differential ultracentrifugation
after isolation, stimulation of proliferation and treatment of MSCs. Autologous T cells isolated from non-adherent
cells were harvested during the procedure to generate MoDCs and then incubated with heat stressed tumor cell lysate
pulsed DCs in the presence of MSC1-derived MVs. T cells were then co-cultured with tumor cells in 96-well plates at
a final volume of 200 μl CM at an effector: target ratio of 100:1 to determine their specific cytotoxic activity. Results:
Flow cytometric analysis, T cell mediated cytotoxicity showed that heat stressed tumor antigen pulsed MoDCs and
MSC1-derived MVs primed T cells elicited non-significantly enhanced cytotoxic activity toward B92 tumor cells
(P≥0.05). Conclusion: These findings may offer new insights into tumor antigen presenting technology involving
dendritic cells and MSC1-derived MVs. Further exploration of the potential of such nanoscale particles in immunotherapy
and in novel cancer vaccine settings appears warranted.- انتشار مقاله: 25-10-1396
- نویسندگان: Nasim Rahmani Kukia,Reza Alipanah-Moghadam,Nowruz Delirezh,Mohammad Mazani
- مشاهده