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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Plant Physiology and Breeding
- نوع مقاله: Journal Article
- کلمات کلیدی: Antioxidants,oxidative stress,Nitric oxide,Zea mays L,Lead stress
- چکیده: آلودگی سرب یک چالش مهم در جهان محسوب می شود. در این پژوهش گیاه ذرت با غلظت های مختلف سرب (صفر، 0.25، 0.75، 1، 1.5، 2.5 و 5 میلی مولار) به مدت ده روز تیمار شدند. نتایج نشان داد که افزایش در غلظت سرب منجر به کاهش رشد، محتوای کلروفیل a و b و افزایش در خسارات اکسیداتیو گردید. کاربرد سرب به طور معنی داری میزان پراکسید هیدروژن را افزایش داد که سطح قابل توجهی از پراکسیداسیون لیپیدها را به دنبال داشت. این تغییرات با افزایش مقدار اکسید نیتریک در غلظت های پایین و کاهش آن در غلظت های بالا همراه بود. کمی افزایش در تولید پراکسید هیدروژن و نیتریک اکسید در غلظت های کم به عنوان پیامبر در مسیرهای سیگنالینگ انتظار می رود که در تحریک عملکردهای دفاعی برای سم زدایی سرب عمل کنند. بنابراین متغیرهای تحمل به تنش گیاهان، از جمله پرولین، اسید اسکوربیک، فلاونوئید و آنتوسیانین ها و فعالیت آنزیم های آنتی اکسیدانی تحت تیمار غلظت های مختلف سرب افزایش می یابد.
- چکیده انگلیسی: Lead pollution is an important issue in the world. In this research maize plants were treated with different concentrations of lead (0, 0. 25, 0.75, 1, 1.5, 2.5 and 5 mM) was applied as lead (II) nitrate for 10 days. The results showed that increase in lead concentration leads to reduction in growth, chlorophyll a and b content and increase in oxidative damages. Application of Pb significantly increased hydrogen peroxide content which followed by a significant level of lipid peroxidation. These changes were accompanied by increase in nitric oxide content in the low and its reduction in the high concentrations. Small increase in generation of hydrogen peroxide and nitric oxide are envisaged at low concentrations as messengers in signaling pathways that may act in triggering defense functions to detoxify lead. Therefore plant’s stress tolerance variables, including proline, ascorbic acid, flavonoid and anthocyanins content and activity of antioxidant enzymes enhanced under different lead applications.
- انتشار مقاله: 17-06-1396
- نویسندگان: Roya Zanganeh,Rashid Jamei
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Plant Physiology and Breeding
- نوع مقاله: Journal Article
- کلمات کلیدی: silver nanoparticles,Magnetic field,Calendula officinalis,Phytochemical compounds
- چکیده: تاثیر میدانهای مغناطیسی و کاربرد نانوذرات نقره بر یک ارگانیسم زیستی، بسته به نوع مواد و سیستم و برنامهی بکار رفته و زمان و شدت کاربرد آن متفاوت است. در سالهای اخیر تحقیقات زیادی در زمینه حساسیت موجودات زنده نسبت به میدانهای مغناطیسی و نانوذرات انجام شده است. به همین منظور، برای ارزیابی تأثیر میدان مغناطیسی و نانوذرات نقره روی فعالیتهای پاداکسایشی گیاه همیشه بهار (Calendula officinalis L.) آزمایشی درسال 1396 در گروه زیست شناسی دانشگاه ارومیه با استفاده از طرح بلوکهای کامل تصادفی با چهار تیمار و سه تکرار انجام شد. تیمارها به این شرح بودند: شاهد، میدان مغناطیسی (با شدت 3mT ) به مدت یک ساعت در روز، نانو ذرات نقره (50 ppm) و ترکیب این دو تیمار. نتایج نشان داد که در گیاهان تحت تیمار با میدان مغناطیسی، نانوذره نقره و میدان مغناطیسی + نانوذره نقره، محتوای فنل کل، فلاونویید و ظرفیت جاروب کنندگی رادیکال آزاد DPPH، میزان فعالیت آنزیمهای فنیل آلانین آمونیالیاز، پلی فنل اکسیداز، کاتالاز، آسکوربات پراکسیداز، گایاکول پراکسیداز و گلوتاتیون ردوکتاز نسبت به گروه شاهد افزایش معنیدار در سطح احتمال 05/0 داشت. تیمار میدان مغناطیسی و نانوذرهی نقره واجد بهترین تأثیر روی وضعیت پاداکسایشی گیاه همیشه بهار بود و میتواند به عنوان جایگزینی مناسب برای ترکیبات شیمیایی مورد استفاده در کشاورزی قرار گیرد.
- چکیده انگلیسی: Effect of magnetic fields (MFs) and silver nanoparticles (SNPs) on a biological organism vary depending on their system and applied materials, time and intensity. In recent years, many studies have been conducted on the sensitivity of living organisms to MFs and NPs. For this purpose, an experiment was carried out in 2016 to study the effects of MF and SNPs on marigold (Calendula officinalis L.) using randomized complete block design with four treatments and three replications. The treatments were as follows: control, MF with B=3 mT for an hour per day, SNPs (50 ppm) and combination of MF (B=3 mT) and SNPs (50 ppm). Results showed that phenolic content, DPPH radical scavenging, phenylalanine ammonia lyase, polyphenoloxidase, catalase, ascorbate peroxidase, guaiacol peroxidase and glutathione reductase activity were significantly increased by the application of MFs and SNPs as compared to the control group. It seems that the MF+SNPs treatment had the best effect on the antioxidant status of C. officinalis L. and may be suitable alternative for chemical compounds used in agriculture.
- انتشار مقاله: 22-08-1396
- نویسندگان: Elham Jalilzadeh,Rashid Jamei,Siavash Hosseini Sarghein
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Plant Physiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Phenolic compounds,Soybean,UV-B,Photosynthetic pigment
- چکیده:
- چکیده انگلیسی: The effects of UV-B radiation on the amount of chlorophyll a, b, carotenoids, flavonoids, anthocyanins, and polyphenols of three soybean cultivars was studied. After germination in the incubator, plants were put in pots of soil and during 3 weeks and irrigated with Hoagland solution every day. After 3 weeks, plants were exposed to UV-B for 20 min every day for a week. In each cultivar, UV-B reduced chlorophyll a, b, and carotenoids and increased anthocyanins, flavonoids, and polyphenols. The reduction of chlorophyll a and b content was significant between two cultivars Williames, Linfored and L17, Linfored. Carotenoids content showed significant decrease between cultivars Linfored, Williames and Linfored, L17. The anthocyanin and flavonoid contents were affected by UV-B and there was a significant difference in the increase between Williames and L17 and also between L17 and Linfored cultivars. Also increase in phenol content was significantly different between Williames and L17 and also between Williames and Linfored cultivars.
- انتشار مقاله: 25-02-1397
- نویسندگان: Farin Nouri,Siavash Hosseini Sarghein,Rashid Jamei
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antioxidant Activity,Phenolic compounds,Correlation,Prunusdomestica L
- چکیده:
- چکیده انگلیسی: Objectives: Consumption of fruits, such as plums and prunes, is useful in treating blood circulation disorder, measles, digestive disorder, and prevention of cancer, diabetes, and obesity. The paper presents a description of antioxidant and antiradical capacity of plum (Prunus domestica L.) in both fresh and dried samples.
Materials and Methods: Samples were mixed with methanol and ethanol (as solvents) and were extracted on magnetic shaker, separately. The experiments were carried out to measure the Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Antioxidant Capacity (TAC), Reducing Power Assay (RPA), Chain Breaking Activity (CBA), and quantity of Malondialdehyde (MDA), 2,2-Diphenyl-1-Picrylhydrazyl (DPPH),Nitric Oxide (NO),Hydrogen peroxide (H2O2) and superoxide(O2-) radicals inhibition.
Results: The results showed that the highest values for the TPC, TFC,TAC, RPA, CBA, DPPH, and NO were related to ethanolic extractsof dried sample which showed statistically significant differences (p2O2 and O2-were related to ethanolic extracts of fresh sample. The correlations data were analyzed among all parameters and the TPC and TFC had a significant correlation (r2=0.977). Moreover, it was found that methanol was more successful in extraction procedure than ethanol (p<0.01).
Conclusion: Findings suggest that the fresh samples are more successful in collecting oxygen free radicals such as superoxide (O2-) and peroxy radicals (ROO.) than dried.- انتشار مقاله: 13-08-1392
- نویسندگان: Amin Morabbi Najafabad,Rashid Jamei
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Flavonoid,Elaeagnus angustifolia,Phenolic compounds
- چکیده:
- چکیده انگلیسی: Objectives: Leaves and flowers ofElaeagnus angustifolia contain phenolic and flavonoid compounds. These compounds have antioxidant properties that protect cells from oxidative damage. The aim of this study was to determine and analyze total phenolic and flavonoid content of leaves and flowers in two E. angustifolia variants using different solvents (ethanol and methanol).
Materials and Methods: Ethanolic and methanolic extracts of the plant leaves and flowers were prepared. Experiments were carried out to measure their phenolic and flavonoid content using two solvents. Data were analyzed using Instat-N software.
Results:Results showed that the amount of phenolic and flavonoid compounds in both ethanolic and methanolic extracts was higher in Fariman variant compared with Mashhad variant. Ethanolic and methanolic extracts of Fariman variant had the highest amount of phenolic compound (10.91±0.18, 10.28± 0.36 mgGAE/100gFW, respectively) and also the highest amounts of flavonoids (5.80±0.10, 3.36±0.05 mgQE/100gFW, respectively). Phenolic and flavonoids compounds were better extracted using methanol and ethanol solvent.
Conclusion: In both varieties and solvents, the amount of phenolic and flavonoids compounds in leaves were higher than flowers. In addition, the phenolic and flavonoids compounds were higher in Fariman compared to Mashhad variants.- انتشار مقاله: 24-06-1392
- نویسندگان: Fereshte Saboonchian,Rashid Jamei,Siavash Hosseini Sarghein
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antioxidant Activity,Temperature,Capsicum annuum L,Phenolic extracts
- چکیده:
- چکیده انگلیسی: Objectives: Sweet peppers Capsicum annuum L. (C. annuum) are an excellent source of vitamins A and C as well as phenolic compounds, which are important antioxidant components that may reduce the risk of diseases. The objective of this study was to evaluate their antioxidant activity under various temperatures.
Materials and Methods: To compare the antioxidant activity in various temperatures (20, 35, 50, and 65 °C), two different types of colored (red and green) sweet bell peppers C annuum were selected. The red peppers were selected from those cultivated in Shahreza, Esfahan and the green peppers with the local name of Gijlar were selected from those cultivated in Urmia, West Azarbayjan. The experiments were carried out to measure the total phenolic and flavonoid content, ferric reducing antioxidant power (FRAP), chain-breaking activity, scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), and hydrogen peroxide radicals.
Results: Total phenol and flavonoid contents of pepper extracts were enhanced with increasing temperature to 65 °C. Scavenging capacity of DPPH radical of red pepper extract was enhanced because of putting at 50 °C for 30 min and for Gijlar pepper extract scavenging capacity was increased at 65 °C. Scavenging capacity of hydrogen peroxide radical of extracts was the highest at 35 °C. Chain-breaking activity of red pepper extract was increased for 60 min at 35 °C. FRAP (C) of red pepper extract was significantly different (p<0.05) in compare with Gijlar pepper.
Conclusion: An appropriate temperature maintained a high antioxidant activity of phenolic compound, which could be due to the combined effect of non enzymatic reaction and phenolic compound stability.- انتشار مقاله: 25-03-1391
- نویسندگان: Narmin I Yazdizadeh Shotorban,Rashid Jamei,Reza Heidari
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Walnut,Juglans regia L,fatty acids,DPPH,Genotype
- چکیده:
- چکیده انگلیسی: Background and aims: Walnuts are good sources of polyunsaturated fatty acids (PUFA) and polyphenols which have beneficial effects such as proper growth, decreasing coronary heart disease, prevention of several kinds of cancer, anti-inflammatory and anti-mutagenic activities. In this study, the fatty acid content and antiradical activity of different walnut (Juglans regia L.) genotypes grown in Kolyaei region located in Kermanshah Province (Iran) were investigated. Methods: In this experimental study, fatty acid compositions in different genotypes of Persian walnut were determined using a GC–FID coupled with a flame ionization detector. For antiradical activity, methanolic extracts of different genotypes affected on DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical. BHA (2-tert-butyl-4-methoxyphenol) was used as the reference compound. Results: Total oil content of walnuts ranged from 63.3 to 78.5%. Oleic acid, linoleic acid, linolenic acid, palmitic acid and stearic acid contents ranged respectively from 17.9 to 28.6%, 46.9 to 56.8%, 10.8 to 13.9%, 5.5 to 7.2% and 2.0 to 3.9%, while trace amounts of other fatty acids (<0.1% each) were detected in the samples. The results demonstrated that fatty acid composition is genotype dependent and the highest amounts of PUFA (due to the high content of linoleic acid) were observed in B2 genotype. Among different studied genotypes, the extract of B2 had also the highest radical scavenging activity and therefore the lowest EC50. Conclusion: It was concluded that pellicle is a necessary protecting layer that can help to inhibit the oxidation of fatty acids.
- انتشار مقاله: 17-05-1393
- نویسندگان: Vali Akbari,Reza Heidari,Rashid Jamei
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Walnut,Juglans regia L,fatty acids,DPPH,Genotype
- چکیده:
- چکیده انگلیسی: Background and aims: Walnuts are good sources of polyunsaturated fatty acids (PUFA) and polyphenols which have beneficial effects such as proper growth, decreasing coronary heart disease, prevention of several kinds of cancer, anti-inflammatory and anti-mutagenic activities. In this study, the fatty acid content and antiradical activity of different walnut (Juglans regia L.) genotypes grown in Kolyaei region located in Kermanshah Province (Iran) were investigated. Methods: In this experimental study, fatty acid compositions in different genotypes of Persian walnut were determined using a GC–FID coupled with a flame ionization detector. For antiradical activity, methanolic extracts of different genotypes affected on DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical. BHA (2-tert-butyl-4-methoxyphenol) was used as the reference compound. Results: Total oil content of walnuts ranged from 63.3 to 78.5%. Oleic acid, linoleic acid, linolenic acid, palmitic acid and stearic acid contents ranged respectively from 17.9 to 28.6%, 46.9 to 56.8%, 10.8 to 13.9%, 5.5 to 7.2% and 2.0 to 3.9%, while trace amounts of other fatty acids (<0.1% each) were detected in the samples. The results demonstrated that fatty acid composition is genotype dependent and the highest amounts of PUFA (due to the high content of linoleic acid) were observed in B2 genotype. Among different studied genotypes, the extract of B2 had also the highest radical scavenging activity and therefore the lowest EC50. Conclusion: It was concluded that pellicle is a necessary protecting layer that can help to inhibit the oxidation of fatty acids.
- انتشار مقاله: 17-05-1393
- نویسندگان: Vali Akbari,Reza Heidari,Rashid Jamei
- مشاهده