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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: RT-PCR,Saudi Arabia,HCC,Golgi glypican73,Sandwich ELISA
- چکیده:
- چکیده انگلیسی: Background: Due to the prevalence of Hepatocellular carcinoma (HCC) in Saudi Arabia, using new markers to
give best diagnostic performance than alpha-feto protein (AFP) are important in early diagnosis. The aim of this work
was to compare the significance between serum and mRNA Golgi glypican73 (GP-73) as newly identified diagnostic
and prognostic markers for HCC among Saudi patients. Materials and Methods: A total of 300 subjects were divided
into: 250 blood samples where 145 samples from HCC, 105 samples from chronic liver cirrhosis (CLC) and 50 normal
controls were investigated for serum GP73 (sGP73) by ELISA. GP-73 mRNA from peripheral blood mononuclear cells
was amplified by RT-PCR. The sensitivity and specificity of both techniques was compared. Results: Serum Golgi
glypican 73 was significantly higher in HCC group compared to cirrhotic and normal controls (p<0.001). Sensitivity
and specificity were 95% for sGP-73, 100% and 90% for Golgi glypican 73 mRNA. The combination of sensitivity
between AFP and sGP73 was 80% and 95% respectively. Conclusion: Both serum Golgi glypican-73 and GP-73Mrna
are good diagnostic biomarkers for early detection of HCC in Saudi patients. RT-PCR is more accurate and sensitive
(100%) than ELISA (95%) in detecting Golgi glypican 73.- انتشار مقاله: 10-05-1397
- نویسندگان: Randa Mohamed Ahmed Farag,Dujana Al Ayobi,Khalid A Alsaleh,Hye-Joo Kwon,Afaf EL-Ansary,Emad Anwar Dawoud
- مشاهده