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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: SCIENTIA IRANICA
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: In this paper, two methods to solve multi-response statistical problems are presented. In these methods, desirability function, genetic algorithm and simulation methodology are applied. The desirability function is responsible for modeling the multi-response statistical problem, the genetic algorithm tries to optimize the model and, finally, the simulation approach generates the required input data from a simulated system. The methods differ from each other in controlling the randomness of the problem. In the first method, replications control this randomness, while, in the second method, the randomness is controlled by a statistical test. Furthermore, these methods are compared by designed experiments and the results are reported.
- انتشار مقاله: 18-05-1385
- نویسندگان: S.H.R. Pasandideh,S.T. Akhavan Niaki
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Guinea Pig,Cytokine,mRNA expression,FMD,Real-time PCR
- چکیده:
- چکیده انگلیسی: Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed
livestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs with
FMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after the
first vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN-
γ, TNF-α and IL-10 expression using relative real-time PCR assay. Our results showed that all of the genes were
upregulated. The expression of TNF-α and IL-10 genes significantly increased (P<0.05) in day 28th in
comparison to the day 7th post the first vaccination. It can be concluded that the vaccine induced immune
responses by increasing expression of the cytokines. Therefore, effects of DNA vaccines on immune system also
may be evaluated using these genes.- انتشار مقاله: 26-10-1392
- نویسندگان: R. Pasandideh,M.R. Nassiri,M. Raouf Delgosha,A.A. Aslaminejad,M. Tahmoorespur,S. Zibaei,M. Doosti,M. Pasandideh
- مشاهده