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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Fisheries Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rainbow trout,Digestive enzymes,Fry development
- چکیده:
- چکیده انگلیسی: The aim of this study was to determine the activity of digestive enzymes during the fry development of Rainbow trout ( Oncorhynchus mykiss ), which has a remarkable role in food digestion and absorption in the first feeding. The assessment of digestive enzymes activity of gastric (pepsin), pancreatic (trypsin, chymotrypsin, α-amylase and lipase) and intestinal (alkaline phosphatase) revealed that enzymes were available on the first day after hatching (DAH) but their activity showed no significant difference from hatching to day 12 (P > 0.05). An increased activity was seen between 12 and 18 DAH and this activity was significantly higher than the first 12 days (P < 0.05). In the primary stages after yolk sac absorption (after 20 to 31 days), enzymes activity showed an increased profile however none of them showed a significant difference between 20 to 31 DAH (P > 0.05). These results could be used as a basis for formulating a suitable feeding and also selecting the best time for starting the feeding so that optimum nutritional values and cost-effectiveness are achieved.
- انتشار مقاله: 12-10-1396
- نویسندگان: F. Golchinfar,A. Zamani,A. Hajimoradloo,R. Madani
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Antigen,Enzyme-Linked Immunosorbent Assay (ELISA),Goat,Przhevalskiana
- چکیده:
- چکیده انگلیسی: Goat warble-fly infestation is one of the parasitic diseases caused by the larvae of przhevalskiana spp. It is known to be a major challenge for health and welfare in infested goats and causes high economic losses in livestock worldwide. The detection of goat grub was previously conducted by direct palpation of second and third stage larvae in the back and flank site of the live animals or visual evaluation of the carcasses in slaughterhouses. However, due to the small size of the first instar larvae of przhevalskiana (less than 1 mm during emerging from the egg), some of the infected cases were ignored and recorded as negative samples. Immunodiagnostic procedures as easy and cost-effective diagnostic methods provide early detection of myiasis in living animals (even when the larvae are still migrating or are undetectable in the animal body).This study was conducted to evaluate the competitive enzyme-linked immunosorbent assay (ELISA) system in order to detect the antibodies of przhevalskiana larvae in the goat sera. In order to prepare the larval antigen, 200 first instar larvae of przhevalskiana were collected from the subdermal region of the back and flanks of the infested goats in Khoramabad slaughterhouse, Khoramabad, Iran, from September 2017 to March 2018. Totally, 37 and 46 sera samples were taken from the infected and uninfected goats. The sensitivity and specificity at cut-off 3SD were obtained at 89.18% and 84.78%. Moreover, the measures of inter-and intra-assay coefficients of variability to express the precision or replicability of ELISA kit results were 5.33% and 2.81%, respectively.
- انتشار مقاله: 12-09-1397
- نویسندگان: A. Bagheri,R. Madani,Sh. Navidpour,N. Hoghoooghi-Rad
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR,Hydatid cyst,DNA extraction,Genetic affinity,Sequencing
- چکیده:
- چکیده انگلیسی: Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran. The genetic diversity of this parasite is very high, particularly in areas where the disease is endemic. It has been suggested in the literature from different parts of the world that diverse factors, such as parasite life cycle, transmission pathways, pathologic disease, immunization, and disease control can be affected by the genetic diversity of the parasite. Various studies indicated sheep strain G1 as the most common genotype throughout the world. This strain is commonly found in the liver and lung repeatedly causing echinococcosis in humans, sheep, and cattle. The present study was conducted to determine the genetic affinity between the protoscolex of E. granulosus in humans and sheep in East Azerbaijan province, Iran for the first time. A total of 120 hydatid cyst samples were collected, 60 of which were from people who referred to the hospitals of East Azerbaijan and 60 were from the sheep slaughtered in Tabriz slaughterhouse. Following DNA extraction, certain regions of the cox1 gene were amplified and evaluated by the polymerase chain reaction. The replicated parts in all isolates had the same size of 450 bp. Electrophoresis was followed by selecting a total of 60 suitable samples, including 30 human samples and 30 sheep samples and sending them for genome sequencing. The overlap of the samples was investigated using the BLAST software. The results of BLAST, sequencing, and overlap demonstrated a genetic linkage of approximately 91.76% between the protoscolex of E. granulosus in human and sheep.
- انتشار مقاله: 23-05-1397
- نویسندگان: S. Zarrabi Ahrabi,R. Madani,B. Shemshadi,Sh. Ranjbar Bahadori,H. Hashemzadeh Farhang
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Mycoplasma,Contagious Agalactiae,P48,Variation,Antigen Heterogeneity
- چکیده:
- چکیده انگلیسی: P48 protein of Mycoplasma agalactiae is used to diagnose infection and was identified as potential vaccine candidate. According to the genetic nature of mycoplasma and variable sensitivity in P48-based serological diagnosis tests, intra species variation of P48 nucleotide sequence investigated in 13 field isolates of difference province of Iran along with three vaccine strains. Samples were collected from sheep and goat and were cultured in modified PPLO broth. Two pair of primer employed to confirm genus and species of isolates and a pair of primer has developed to amplify the P48 gene. The sequencing results of PCR products were aligned and analyzed besides published sequences in GenBank. T-Cell and B-Cell epitopes and antigenicity of sequence were computationally predicted. The results have shown P48 nucleotide sequences are 99.9% identical in field isolates and vaccine strain of Iran, but analysis of GenBank published sequences have shown divergence up to 5.3% at the nucleotide level and up to 4.9% divergence in protein level of P48 sequences of Iran isolates and other available sequences in GenBank. Single nucleotide polymorphism exists in 89 positions and variable amino acid was observed at 25 residues. Phylogenetic analyses have shown that Mycoplasma agalactiae isolates fall into three main groups based on P48 nucleotide sequences. Immunoinformatics analysis of all available P48 nucleotide sequences have revealed that gene variation lead to differences in immunological properties, but the gene in Iranian isolates are conservative and stable. The sequence variation in epitopes can be underlying source of antigen heterogeneity as a result, affect serological tests accuracy. Due to the high level of divergence in worldwide isolates and high degree of similarity in P48 protein of Iranian isolates, designing recombinant P48 protein based on local pattern can increase the sensitivity and consistency of serological test.
- انتشار مقاله: 21-05-1396
- نویسندگان: P. Panahi,S. A. Pourbakhsh,T. Zahraei Salehi,M. Esmaelizad,R. Madani
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,Avian Influenza,Gold nanoparticle
- چکیده:
- چکیده انگلیسی: Influenza is one of the most important viral diseases that is common among the birds and the mammals
and is caused by specific viruses that belong to the Orthomyxoviridae family. Migratory aquatic birds are
the reservoir of the disease and there is a likelihood of the disease in any region. There are different
methods for detecting the avian influenza, but by the point of detection rates, the ELISA may be one of
the most important current methods. In this work we synthesized Gold nanoparticles and conjugated it
with rabbit-anti-chicken IgG-HRP. An ELISA test was done to compare the bioactivity of Au-antichicken
HRP with anti-chicken HRP in order to detect the antibody against the H9N2 subtype of avian
influenza virus. Using 133 field chicken sera, the sensitivity of nano-ELISA as compared to traditional
ELISA was calculated to be 100%, whereas the specificity was 92%. This method was significantly more
sensitive than the traditional ELISA and didn’t require extra costs. It can therefore be concluded that the
AuNP-HRP conjugate can be applicable in immune analysis procedure where a more confident result is
required.- انتشار مقاله: 20-12-1393
- نویسندگان: S. Imani Gheshlaghchaei,R. Madani,F. Golchinfar,T. Emami,H. Gholami
- مشاهده