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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: HCC- Hepatocellular Carcinoma,LC- Liver Cirrhosis,miR- micro RNA,RT PCR- Real time Polymerase Chain Reaction,Ct -Threshold cycle
- چکیده:
- چکیده انگلیسی: Background/objective: HCC is a multistep process starting from chronic hepatitis that progress through cirrhosis
to HCC. MicroRNA expression level was found to be deregulated in HCC. To find out whether the expression level of
miR-34a and miR-183 was deregulated in HCC compared to controls without HCC. Methods: Real time quantitative
PCR was done to find out the miRNA expression level in terms of Ct value followed by statistical analysis. Results:
Over-expression of miR-183 and under-expression of miR-34a in HCC was detected. All changes in expression level
of miR-34a and miR-183 were found to be due to HCC compared to controls without HCC. So both miR-34a and
miR-183 were suitable to differentiate HCC from Cirrhosis and chronic hepatitis with an efficient diagnostic power of
sensitivity, specificity and expression level. But they might not have any role in patients’ survival. Conclusion: miR-
34a and miR-183 might be considered as potential markers of HCC screening molecule in addition to other approved
panel of marker. Our study warrants further expression level study.- انتشار مقاله: 28-10-1396
- نویسندگان: Dipu Bharali,Hakim B Jebur,Debabrat Baishya,Suresh Kumar,Manash P Sarma,Mirza Masroor,Juheb Akhter,Syed A Husain,Premashis Kar
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: HCC,HBV,genotype C,North East India,South India and North India
- چکیده:
- چکیده انگلیسی: Background and Objectives: Newer genotypes of HBV have been reported from India. This study was aimed to
determine the circulating genotypes of HBV in hepatocellular carcinoma patients from three different geographical
locations of India. Methods: 141 HBV related HCC cases were included from three different hospitals across the country.
Genotyping of HBV was performed by PCR using type specific primers specially designed in 70 cases. Samples of
interest were confirmed by direct sequencing of the precore/core region of HBV genome. Results: Genotypes could
be detected in 40 (57.14%) out of the 70 HBV DNA positive HCC cases by type specific primers. HBV genotype D
was documented in 20 (50%), genotype A in 10 (25.0%) and genotype C in 10 (25.0%) of these HCC cases. Genotype
C of HBV was detected only in the samples from North East India. No significant difference was observed for the
biochemical profile. Conclusion: Although Genotype D is the major HBV genotype in India followed by A, detection
of HBV genotypes C in HCC patients indicates a changing epidemiology of the virus in India that may require region
based management of the virus.- انتشار مقاله: 26-10-1396
- نویسندگان: Manash P Sarma,Minakshi Bhattacharjee,Premashis Kar,Subhash Medhi
- مشاهده