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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Glioblastoma,Invasion,migration,astaxanthin,matrix metalloproteinase
- چکیده:
- چکیده انگلیسی: Objectives: The study was to investigate anti-migration and invasion effects of astaxanthin (ATX), a natural carotenoid derivative distributed in marine environments, against A172 human glioblastoma cells. Materials and Methods: Cell viability after ATX treatment was measured by MTT assays. Tumor cell migration and invasion were observed by scratch and Boyden chamber assays, respectively. Expression of MMP-2 and activity of MMP-9 were observed by immunoblotting and gelatin zymography, respectively. Results: ATX up to 150 µM was not toxic to A172 cells at 48 h post-treatment. In contrast, ATX at 50 and 100 µM significantly decreased migration and invasion of A172 cells at 24 and 48 h post-treatment. Metastatic-reducing effect of ATX is associated with the reduction of MMP-2 and MMP-9 expressions in a dose-dependent manner. Conclusion: This finding indicated that ATX has anti-migration and invasion effects against human glioblastoma cells and might be applicable for the protection against metastasis of glioblastoma.
- انتشار مقاله: 07-12-1398
- نویسندگان: Tanapan Siangcham,Pornpun Vivithanaporn,Kant Sangpairoj
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Breast cancer,Proliferation,montelukast,zafirlukast
- چکیده:
- چکیده انگلیسی: Despite a discovery of hormonal pathways regulating breast cancer, a definitive cure for the disease requires further
identification of alternative targets that provide a hormone-independent support. Apart from their role in inflammatory
diseases, cysteinyl leukotriene (CysLT) receptor antagonists (LTRAs) decrease the risk of lung cancer in asthma patients
and inhibit tumor progression in several malignancies. In the present study, we evaluate the effects of two chemically
different, clinically relevant LTRAs (montelukast and zafirlukast) in a triple negative breast cancer cell line, MDAMB-
231. We found that these two LTRAs reduced breast cancer cell viability in a dose-dependent manner with the
50% inhibitory concentration (IC50) between 5-10 μM. Although both LTRAs have several pharmacological properties
in common, we noticed that montelukast mainly induced apoptosis, while zafirlukast mainly exerted its action on cell
cycle. However, the precise mechanisms responsible for such different effects remain unclear. In summary, our results
suggest that CysLT plays a role in proliferation and survivability of breast cancer cells in the absence of hormonal stimuli.- انتشار مقاله: 15-08-1396
- نویسندگان: Kran Suknuntha,Ruedeemars Yubolphan,Kanokpan Krueaprasertkul,Sirada Srihirun,Nathawut Sibmooh,Pornpun Vivithanaporn
- مشاهده