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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Antiproliferative,Dolichol,Nypa fruticans,mangrove
- چکیده:
- چکیده انگلیسی: Background: Secondary metabolites from the group of isoprenoid compounds are widely distributed in mangrove
plants. Polyisoprenoids (dolichol and polyprenol) are known to have benefits as anticancer agents. The present study
was conducted to determine the cytotoxic potential of polyisoprenoids in leaves from seventeen selected mangrove
species against colon cancer (WiDr) cells. Methods: Cytotoxic activity was evaluated by MTT assay in vitro using
WiDr human colon cancer cells and 3T3 fibroblasts from Swiss albino mouse embryo tissue as controls. Mechanisms
of action were approached by assessing apoptosis and the cell cycle using flow cytometry and fluorescence microscopy
with annexin V-FITC, as well as expression of Bcl-2 and cyclin D1 by immunocytochemistry. Results: Polyisoprenoids
from N. fruticans leaves demonstrated the highest anticancer activity, with an IC50 of 180.2 μg/mL, as compared to
397.7 μg/mL against 3T3 normal cells. Significant decrease in the expression of Bcl-2 and cyclin D1 was also noted,
facilitating apoptosis and arrest of the cell cycle in the G0-G1 phase in WiDr cells. The present study showed for the
first time that polyisoprenoids from N. fruticans exhibit concrete anticancer activity in vitro, decreasing cell proliferation
and inducing apoptosis in colon cancer cells. Conclusions: Polyisoprenoids isolated from N. fruticans leaves may have
promise as a source of anticancer agents.- انتشار مقاله: 04-09-1396
- نویسندگان: Dini P Sari,Mohammad Basyuni,Poppy AZ Hasibuan,Sumardi Sumardi,Arif Nuryawan,Ridha Wati
- مشاهده