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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Autophagy,Cyclophosphamide,Leydig cells,Squid ink polysaccharide
- چکیده:
- چکیده انگلیسی: Objective(s): The aim of this study was to explore the effects of Squid ink polysaccharide (SIP) on prevention of autophagy and oxidative stress induced by cyclophosphamide (CP) in Leydig cells of mice.
Materials and Methods: Examination of reproductive organ exponents, abnormal sperm rate, activities of superoxide dismutase (SOD), catalase (CAT), contents of malondialdehyde (MDA), and histological structure were performed to detect the optimal dose of SIP against oxidative stress damage in vivo, and autophagy-associated protein LC3 and Beclin-1 were examined by immunofluorescence, and their expression was detected by Western blot analysis. Leydig cells ultrastructural changes were observed by transmission fluorescent microscope.
Results: SIP significantly inhibited sperm aberration, histological structure and injury of seminiferous tubules caused by CP, as well as the antioxidant activity of SOD and CAT were increased; contents of MDA were decreased. The optimal dose of SIP for prevention of oxidative stress injury by CP was 80 mg/kg. In addition, LC3 and Beclin-1 fluorescent granules were much less in the Leydig cell layer after treatment via SIP compared with the CP-treated group, and the expression levels of LC3 and Beclin-1 were also decreased. Furthermore, characteristics of cell autophagy such as mitochondrial swelling, autophagic vacuoles, and chromatin pyknosis were observed in CP-treated Leydig cells, but SIP could effectively weaken injury of Leydig cell ultrastructure by CP.
Conclusion: SIP, as an antioxidant, prevents the cytoskeleton damage through up-regulation antioxidant capacity and inhibition autophagy caused by CP.- انتشار مقاله: 23-07-1396
- نویسندگان: Yi-Peng Gu,Xiao-Mei Yang,Zhen-Hua Duan,Jiang-Hua Shang,Ping Luo,Wei Xiao,Da-Yan Zhang,Hua-Zhong Liu
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Testis,Mice,Cyclophosphamide,Nrf2/ARE,Squid Ink Polysaccharides
- چکیده:
- چکیده انگلیسی: Objective(s):Cyclophosphamide (CP) toxicity on testis was hampered by squid ink polysaccharide (SIP) via restoration of antioxidant ability in our previous investigations. This study investigated roles of Nrf2/ARE signal pathway in testis of treated mice. Materials and Methods: Male Kunming mice were employed to undergo treatment with SIP and/or CP. Protein levels of Nrf2, keap-1, histone deacetylase 2 (HDAC2), quinone oxidoreductase 1 (NQO-1), and heme oxygenase 1 (HO-1) and phosphorylation level of protein kinase C (PKC) in testis were evaluated by Western blotting. Results: Data showed that SIP elevated expressions of NQO-1 and HO-1 genes, two downstream target molecules of Nrf2, via activating Nrf2 to play preventive roles on CP-treated testis, and further discovered that upstream regulators of Nrf2, keap-1, HDAC2, and PKC, were concerned with the regulation of Nrf2. Conclusion: These results suggest that SIP could effectively weaken CP-associated testicular damage via Nrf2/ARE signal pathway.
- انتشار مقاله: 03-06-1394
- نویسندگان: Xiaoyan Le,Ping Luo,Yipeng Gu,Yexing Tao,Huazhong Liu
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Oxidation,polyphenol,Drilling fluid,Lignin,Thinner
- چکیده:
- چکیده انگلیسی: In this work, a novel thinner derived from natural botany Chinese Larch Tree was obtained by combination lignin with phenol using H2O2 as oxidant and the acting efficiency of lignosulfonate in the drilling fluids is greatly enhanced after oxidation with H2O2. Both the chemical structure and the adsorptive ability of the oxidation product are investigated. From experimental results, it was shown that lignin couple with polyphenol and the combination was greatly improved during the process of oxidation under H2O2 in light of the increased molecular weight of the derivative and different major function groups content after oxidation. Furthermore, the oxidized derivative has the good adsorptive-warping ability on the outer surface of particulate clay, which gives great potential for developing novel polymer drilling fluid with good inhibitory and thinning properties.
- انتشار مقاله: 18-06-1394
- نویسندگان: Jie Zhang,Zhang Fan,Zhongzheng Lee,Pingya Luo,Ayodeji A. Jeje,Gang Chen
- مشاهده