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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Echocardiography,Scaffold,Ejection fraction,mesenchymal stem cell,fractional shortening
- چکیده:
- چکیده انگلیسی: BACKGROUND: Bone marrow-derived mesenchymal cells
can transdifferentiate into Cardiomyocyte cells and improve
heart function after transplantation. Since biomaterials can
improve the cell retention in the site, cell survival and differentiation,
heart tissue engineering is now being explored as an
applied solution to support cell-based therapies and increase
their efficacy for myocardial diseases. Chitosan in combination
with Glycerol Phosphate (GP) can produce a thermo sensitive
material that in body temperature can form a jellylike material.
OBJECTIVES:The aim of this study was to evaluate the effects of
a combination of autologous undifferentiated bone marrow
mesenchymal stem cells (MSCs) and injectable scaffold on cardiac
function improvement in rabbits after inducing myocardial
infarction. METHODS: The Left Anterior Descending (LAD)
coronary artery was ligated by No. 6-0 polyamide suture
material, and autologous MSCs with injectable scaffold were
injected into the margins of the infarcted zone at the time of
surgery. At 4 weeks after transplantation, the cardiac function
and structure was detected using echocardiography. RESULTS:
There was no significant difference among the three groups (MI
only, MI Scaffold, and MI+Scaffold+MSCs) in the Echocardiographic
parameters including, heart rate (HR), Ejection Fraction
(EF), Fractional Shortening (FS), Left Ventricular Diameter
(LVD) and Left Ventricular Parietal Wall Diameter (LVPW).
CONCLUSIONS: A combination of autologous undifferentiated
bone marrow MSCs and injectable scaffold made of Chitosan+
Glycerol Phosphate in echocardiographic evaluation did not
have a positive influence on achieving functional improvement.- انتشار مقاله: 24-07-1391
- نویسندگان: Nazanin Jafari,Mohammad Mahdi Dehghan,Mohammad Abarkar,Mohammad Hejazi,Pegah Abbasnia,Mohammad Molazem,Amir Tavakoli,Rouh-allah Mehdinavaz Aghdam,Seyed Hosein Ahmadi Tafti,Parviz Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Bone marrow,mesenchymal stem cell,Equine
- چکیده:
- چکیده انگلیسی: Objective- To evaluate growth characteristics and differentiation capacity of equine mesenchymal stem cell (eMSCs) derived from bone marrow (BM).
Study design- In vitro experimental study.
Animals- Four young adult horses (2-5 years old)
Procedure- Cell morphology and growth characteristics of eMSCs harvested from BM were evaluated in standard culture conditions. eMSCs in passage 3 were subjected to osteogenic and adipogenic differentiation induction to investigate their differentiation potential.PCR analysis of differentiated cells was done to determine differentiation.
Results-The cells expanded to sufficient quantity for therapeutic purposes within days and they survived to later passages while sustained their fibroblast-like morphology. Positive osteogenesis was detected via Alizarin Red staining and adipogenesis was confirmed by Oil Red O staining. The cells were more potential to differentiate into osteoblasts rather than adipocytes. PCR analysis approve relative expression of osteogenic and adipogenic genes.
Conclusions and Clinical relevance - The results of this study further support eBM-MSCs as a cell population with the capacity to proliferate and differentiate down the osteogenic and adipogenic lineages. Equine BM is a potentials source of MSCs for cell-based regenerative therapies in horse.
- انتشار مقاله: 28-04-1393
- نویسندگان: Elnaz Azad,Farajollah Adibhashemi,Mohammad Mehdi Dehghan,Seyed Mehdi Ghamsari,Reza Sedaghat,Seyed Mehdi Nasiri,Pegah Abbasnia
- مشاهده