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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Testosterone,Extracellular matrix,Bovine testicular cells
- چکیده:
- چکیده انگلیسی: Testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. Given that extracellular matrix (ECM) facilitates differentiation of spermatogonial stem cells (SSCs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ECM on SSCs differentiation. In experiment 1, testosterone production was measured in testicular cells cultured for 12 days on ECM or plastic (control). In experiment 2, testosterone production was assessed in testicular cells cultured on ECM or plastic (control) and exposed to different concentrations of hCG. In experiment 3, the gene expression of factors involved in testosterone production was analyzed. Testosterone concentration was lower in ECM than in the control group in experiment 1 (p < 0.05). In experiment 2, testosterone concentration was increased in response to hCG in both groups but cells cultured on ECM were more responsive to hCG than those cultured on plastic (p < 0.05). In the experiment 3, qRT-PCR revealed the inhibitory effect of ECM on the gene expression of steroidogenic acute regulatory protein (StAR) (p < 0.05). Nevertheless, the expression of LH receptor was greater in ECM-exposed than in unexposed cells (p < 0.05). In conclusion, the present study showed that inhibiting the expression of StAR, ECM could lower testosterone production by Leydig cells during in vitro culture. In addition, the results indicated that ECM could augment the responsiveness of Leydig cells to hCG through stimulating the expression of LH receptor.
- انتشار مقاله: 24-11-1395
- نویسندگان: Vahid Akbarinejad,Parviz Tajik,Mansoureh Movahedin,Reza Youssefi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: bovine,Differentiation,Spermatogonial stem cells,Fibroblast growth factor receptors
- چکیده:
- چکیده انگلیسی: The receptors 1 and 2 of fibroblast growth factor (FGFR1 and FGFR2, respectively) have been observed in all types of testicular cells. Culture on extracellular matrix (ECM) has been observed to lead to initiation of differentiation in spermatogonial stem cells (SSCs). The present study was carried out to investigate whether FGFR1 and FGFR2 play a role in SSCs differentiation. Following isolation, bovine testicular cells were cultured on ECM-coated or uncoated (control) plates for 12 days. The gene expression of THY1, cKIT, FGFR1 and FGFR2 was evaluated using quantitative real-time polymerase chain reaction (PCR). Results related to the gene expression of markers of with undifferentiated (THY1) and differentiated (cKIT) spermatogonia implicated stimulation of self-renewal and differentiation in cells cultured on ECM-coated and uncoated plates, respectively (p < 0.05). Concomitantly, the expression of FGFR2 increased during culture in the ECM group (p < 0.05), whereas it did not change in the control group (p > 0.05). As a result, the gene expression of FGFR2 was greater in the ECM than control group (p < 0.05). Nevertheless, FGFR1 expression did not change during culture in the control and ECM groups (p > 0.05). In conclusion, the present study revealed the potential role of FGFR2 in differentiation of SSCs during culture on ECM.
- انتشار مقاله: 09-03-1395
- نویسندگان: Vahid Akbarinejad,Parviz Tajik,Mansoureh Movahedin,Reza Youssefi
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: bovine,Co-culture,FSH,Sertoli,SSCs
- چکیده:
- چکیده انگلیسی: The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be increased in vitro, at first. Follicle stimulating hormone (FSH) has been suggested to play a determinant role in the survival of germ cells in addition to increasing spermatogonial proliferation. In this study, the in vitro effects of FSH on spermatogonial cell colony formation were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. The identity of the Sertoli cells and spermatogonial stem cells were confirmed through immunocytochemistry and colony morphology, respectively. Co-cultured Sertoli and spermatogonial cells were treated with FSH in different dose of 10, 20 and 40 IU mL-1 FSH, before colony assay. Results indicated that, FSH increased in vitro colonization of spermatogonial cells in comparison with control group. In conclusion, using FSH provided proper bovine spermatogonial stem cell culture medium for in vitro study of these cells.
- انتشار مقاله: 24-12-1391
- نویسندگان: Reza Narenji Sani,Parviz Tajik,Mohammad Hassan Yousefi,Mansoureh Movahedin,Babak Qasemi-Panahi,Shiva Shafiei,Mahmood Ahmadi Hamedani
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Citric acid,Epididymal sperm,Citrate-Protoplasmic Droplet
- چکیده:
- چکیده انگلیسی: For evaluation of citric acid and citrate effects on bovine epididymal protoplasmic droplets, fifty bovine testes were collected in the October 2007 till June 2008 from Urmia slaughterhouse and transported to the laboratory in a cool container filled with 5 °C ice pack. Caudal epididymis was incised and sperm cells were put into Petri dishes containing hams f10 media with 10% fetal calf serum (FCS), which were kept in 37 °C, CO2 incubator. Then sperm cells were counted and 50-milion per mL concentration was prepared. After this stage, three dilutions of citric acid (0.1, 0.2, 0.3 N) and one dilution of citrate (1N), based on normal osmolarity and normal pH were added to a micro tube containing 25 milion per mL sperm. Then one-step eosin-nigrosin staining in 30-60-120-240-360 minutes was performed and slides were evaluated with 1000-x phase contrast microscope and 200 sperm cells per slide were counted. The results revealed significant difference between blank and citric acid 0.3 N. The proportion of protoplasmic droplet in group consisting of 0.3 N acid citric in 120-240-360 minutes, was significantly lower than that of blank (P < 0.05). There was no significant difference between citrate – blank and citric acid 0.1N-blank groups, but after 240 minutes significant difference was observed between blank & citric acid 0.2 N (P < 0.05). In conclusions citric acid based on dilution and time duration can reduce the proportion of bovine epididymal sperm cytoplasmic droplets.
- انتشار مقاله: 08-10-1391
- نویسندگان: Keivan Abdy,Parviz Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Cat,Follicular ovarian cyst,cystic ovarian disease
- چکیده: در این گزارش، تشخیص و درمان یک مورد کیست تخمدانی فولیکولی در یک گربه پرشین ماده 5 ساله شرح داده میشود. گربه مورد نظر در ملامسه ناحیه پشت کمر، موقعیت لوردوزیس را به خود گرفته و پاهای عقب را بالا و پایین حرکت میداد.بررسی سونوگرافی ناحیه شکم دو کیست در تخمدان چپ گربه نشان داد. ارزیابی استروژن سرم خون، غلظت بالای 17 بتا_استرادیول (pg/mL 105) را نشان داد. با این حال غلظت پروژسترون نرمال (ng/mL 3/0) بود. بر این اساس، گربه مبتلا به کیست فولیکولار فعال تشخیص داده شد. حیوان مذکور با گنادوتروپین جفتی انسان (hCG) به صورت داخل عضلانی تحت درمان قرار گرفت. 30 روز پس از تجویز hCG، یک تزریق گنادوتروپین جفتی اسب (eCG) (50 واحد بین المللی) به صورت داخل عضلانی انجام شد. جفتگیری طبیعی با یک گربه نر پرشین بارور انجام گرفت. در نتیجه، به نظر میرسد که درمان دارویی کیستهای فولیکولار فعال را میتوان به منظور حفظ باروری در گربه سانان به ک ار گرفت.
- چکیده انگلیسی: In the present report, diagnosis and treatment of a case with follicular ovarian cysts in a 5-year-old Persian queen cat is described. In response to palpation of spines, the queen cat presented herself in lordosis and danced up and down with her rear legs. Trans-abdominal ultrasonography examination showed 2 cysts in the left ovary of the queen. Serum estrogen assay indicated elevated level of 17 β-estradiol concentration (105 pg/ml). However, progesterone concentration was normal (0.3 ng/ml). Accordingly, the queen was diagnosed with functional follicular cysts. The queen was treated with an administration of hCG intra-muscularly. Thirty (30) days after the administration of hCG, an injection of equine chorionic gonadotropin (eCG) (50 IU) was given intra-muscularly. Natural mating was done with a fertile Persian tom cat. In conclusion, it seems that treatment of functional follicular cysts can be applied to preserve fertility in cats.
- انتشار مقاله: 22-10-1393
- نویسندگان: Reza Youssefi,Parviz Tajik,Vrya Tohidi,Vahid Akbarinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Spermatogonial stem cell,CSF1,bovine spermatogonia,SSC coculture,SSC niche
- چکیده: زمینه مطالعه: سلولهای بنیادی اسپرماتوگونی (SSCs) گروه کوچکی از سلولهای اسپرماتوگونی نوع A هستند که می توانند سلولهایی کاملاً مشابه خود ایجاد نموده (خودسازی) و همچنین تمایز یابند و در پایان روند اسپرماتوژنز، اسپرم را تولید نمایند. فرآیند خودسازی SSCs به طور کامل شناخته نشده است. برای فراهم شدن امکان مطالعه خصوصیات و عملکرد این سلولها که منجر به خودسازی و تمایز آنها می گردد، دسترسی به تعداد کافی از آنها ضروری است. هدف: در این مطالعه اثر دوزهای مختلف CSF1 روی کلونیزاسیون SSCs در همکشتی با سلولهای سرتولی بررسی شد. CSF1 از عواملی است که پیشنهاد شده ممکن است در SSC niche (جایگاه قرارگیری SSCs در لوله های سمینیفروس) که در آن خودسازی به تمایز برتری دارد، نقش داشته باشد. روش کار: تعداد و مساحت کلونی های اسپرماتوگونی ایجاد شده در حضور دوزهای متفاوت CSF1 (0، 10، 50 و 100 ng/ml) طی روزهای 4، 7 و 11 کشت ارزیابی گردید. توسط رنگ آمیزی ایمیونوسیتوفلورسنت علیه مارکرهای OCT4 و vimentin ماهیت SSCs و سلولهای سرتولی تاٌیید شد. نتایج: تعداد کلونی ها از روز 4 تا روز 11 در تمامی گروهها مستقل از حضور و یا دوز CSF1 به شکل معنی داری افزایش نشان داد. مشاهده گردید که تعداد کل کلونی ها و مجموع مساحت آنها در گروههای SCF1 10 و 50 ng/ml نسبت به گروههای CSF1 100 و 0 (کنترل) بالاتر است اما این تقاوت تنها در مورد مقایسه مجموع مساحت کلونی ها بین گروه CSF1 10 ng/ml و کنترل و تنها در روز 4 معنی دار بود (p
- چکیده انگلیسی: BACKGROUND: Spermatogonial stem cells (SSCs) are infrequent
self-renewing cells among the type A spermatogonia
within the seminiferous tubules and are the basis of spermatogenesis
in mammalian testis. An adequate number of SSCs is a
primary requirement for the study of their behavior, regulation, and
further biomanipulation. OBJECTIVES: In this paper, we studied
the development of the primary co-cultures of type A spermatogonia
and prepubertal bovine sertoli cells in the presence of Colony
Stimulating Factor 1 (CSF1), a potential contributor in the SSC
niche. METHODS: The effect of different concentrations of CSF1
(0, 10, 50 and 100 ng/mL) on the colonization activity of spermatogonial
cells was assessed 4, 7 and 11 days after the beginning of the
culture by counting the total number of colonies and measuring their
area in each group of the present experiment. Immunofluorescent
staining against OCT4 and vimentin led to the confirmation of the
nature of both the SSCs and sertoli cells. RESULTS: Results showed
that the total number of colonies from day 4 to 11 increased
significantly in all groups, independent of CSF1 concentration. In
addition, the total number and total area of colonies were higher (not
significant) in 10 and 50 ng/mL CSF1 treatments than the control
and 100 ng/mL CSF1 groups in all the three evaluations during the
experiment. However, this difference was only significant (p<0.05)
between the total area of colonies in the control and 10 ng/mLCSF1
groups at day 4 of co-culture. CONCLUSIONS:It was concluded that
CSF1 can be a suitable growth factor for improving SSCs colonization
in vitro, particularly during the first days of culture where
accompanying sertoli cells still have not proliferated sufficiently to
support the propagating spermatogonial cells.- انتشار مقاله: 15-08-1391
- نویسندگان: Shiva Shafiei,Parviz Tajik,Hamid Ghasemzadeh-nava,Mansoureh Movahedin,Massoud Talebkhan Garoussi,Babak Qasemi-Panahi,Peyman Rahimi Feyli
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Echocardiography,Scaffold,Ejection fraction,mesenchymal stem cell,fractional shortening
- چکیده:
- چکیده انگلیسی: BACKGROUND: Bone marrow-derived mesenchymal cells
can transdifferentiate into Cardiomyocyte cells and improve
heart function after transplantation. Since biomaterials can
improve the cell retention in the site, cell survival and differentiation,
heart tissue engineering is now being explored as an
applied solution to support cell-based therapies and increase
their efficacy for myocardial diseases. Chitosan in combination
with Glycerol Phosphate (GP) can produce a thermo sensitive
material that in body temperature can form a jellylike material.
OBJECTIVES:The aim of this study was to evaluate the effects of
a combination of autologous undifferentiated bone marrow
mesenchymal stem cells (MSCs) and injectable scaffold on cardiac
function improvement in rabbits after inducing myocardial
infarction. METHODS: The Left Anterior Descending (LAD)
coronary artery was ligated by No. 6-0 polyamide suture
material, and autologous MSCs with injectable scaffold were
injected into the margins of the infarcted zone at the time of
surgery. At 4 weeks after transplantation, the cardiac function
and structure was detected using echocardiography. RESULTS:
There was no significant difference among the three groups (MI
only, MI Scaffold, and MI+Scaffold+MSCs) in the Echocardiographic
parameters including, heart rate (HR), Ejection Fraction
(EF), Fractional Shortening (FS), Left Ventricular Diameter
(LVD) and Left Ventricular Parietal Wall Diameter (LVPW).
CONCLUSIONS: A combination of autologous undifferentiated
bone marrow MSCs and injectable scaffold made of Chitosan+
Glycerol Phosphate in echocardiographic evaluation did not
have a positive influence on achieving functional improvement.- انتشار مقاله: 24-07-1391
- نویسندگان: Nazanin Jafari,Mohammad Mahdi Dehghan,Mohammad Abarkar,Mohammad Hejazi,Pegah Abbasnia,Mohammad Molazem,Amir Tavakoli,Rouh-allah Mehdinavaz Aghdam,Seyed Hosein Ahmadi Tafti,Parviz Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی:
- انتشار مقاله: 24-07-1386
- نویسندگان: Mohammad Rostaei,Gholamreza Nikbakht Brujeni,Ali Baghbanzadeh,Parviz Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی:
- انتشار مقاله: 08-01-1384
- نویسندگان: Mohammad Hosein Motedayen,Fatemeh Todehdehghan,Parviz Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Rabbit,Stem cells,defect,PVA,cartilage
- چکیده:
- چکیده انگلیسی: Objective- To evaluate the biological compatibility of differentiated stem cells embedded in poly-vinyl-alcohol (PVA) scaffolds for repair of distal femoral cartilage defect. Design- Experimental in vivo study. Animals- Twelve adult male New Zealand white rabbits were used which were divided into two groups (I, II) six rabbits each. Procedures- Mesenchymal stem cells were isolated from humerus bone marrow of group I rabbits and were cultured and differentiated on PVA scaffolds to chondrocytes. Scanning Electron Microscopy (SEM) showed well distribution of the cells inside the scaffold. A 4 mm diameter full thickness cartilage defect was created on central region of bilateral distal femoral joint surface (patellar groove) in all rabbits. In group (I) the defects were covered with autologous differentiated MSCs-seeded scaffolds; whereas the group II rabbits were left without any treatment as control ones. One month and three months after operation, three rabbits were sacrificed from each group, randomly. Histopathologic evaluation of defects was performed with H&E and trichrome staining. Results- The findings showed that in the engineered cartilage with the PVA scaffold, the defects were filled with smooth, shiny white tissue macroscopically at three months after the transplantation. Despite much connective tissue formed in defect area after three months, there was no evidence of chondrocytes in control group, whereas the defects of experimental group were almost completely filled with hyaline cartilage. Conclusion and Clinical Relevance- The results indicated there is positive possibility for partial resurfacing of cartilage defect using stem cell-seeded PVA scaffolds
- انتشار مقاله: 23-05-1392
- نویسندگان: Davood Sharifi,Pejman Mortazavi,Mohammad Mehdi Dehghan,Parviz Tajik,Mohammad Abedi,Masoud Soleimani
- مشاهده