در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Neonatology
- نوع مقاله: Journal Article
- کلمات کلیدی: Polymorphism,Thrombophilia,Recurrent pregnancy loss
- چکیده:
- چکیده انگلیسی: Background: Recurrent pregnancy loss (RPL) is a common problem among couples, and acquired thrombophilia is the well-known etiology of RPL. The aim of this study was to establish the association between inherited thrombophilic gene polymorphisms and RPL.
Methods: This case-control study was conducted on 50 women with unexplained RPL and 50 parous women with no history of miscarriage (age range: 17–48 years). The data were collected during 2013–2015 in Sarem Hospital, Tehran, Iran. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for glycoprotein IIIa (PLA1/PLA2), KDR (Q472H), and β-fibrinogen (-455G/A); tetra-primer amplification refractory mutation system (ARMS)-PCR for glycoprotein Ia (807c/t) and vascular endothelial growth factor (VEGF) (2578c/a), and ins/del PCR for angiotensin I-converting enzyme (ACE) (intron 16 I/D). The association between the frequency of the genotypes and RPL was determined by Chi-square and Fisher’s exact tests.
Results: The results of the present study revealed a significant relationship between glycoprotein Ia (807C/T), VEGF (2578C/A), and ACE (intron 16 I/D) polymorphisms and RPL (P=0.00, 0.02, and 0.00, respectively). In contrast, no relationship was observed between β-fibrinogen (-455G/A), KDR (Q472H), and glycoprotein IIIa (PLA1/PLA2) polymorphisms and increased risk of RPL (P>0.05).
Conclusion: This study demonstrated that glycoprotein Ia (807C/T), VEGF (2578C/A), and ACE (intron 16 I/D) polymorphisms may be a risk factor for the women with a history of RPL.
- انتشار مقاله: 29-09-1396
- نویسندگان: Parisa Maziri,Golnaz Asaadi Tehrani,Fereshteh Bahrami Hidagi,Masoumeh Nejatollahi,Sedigheh Asadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: DNA Methylation,PTEN,Endometrial neoplasms,APC
- چکیده:
- چکیده انگلیسی: Background: Endometrial neoplasms is one of the most typical gynecologic diseases with harmful effects. Promoter
hypermethylation is an important mechanism of the inactivation of tumor suppressor genes in endometrial neoplasms.
Epigenetic changes of the PTEN and APC genes have shown to be present in various cancers. Therefore, in this study,
we have investigated the association between the promoter hypermethylation of PTEN and APC genes with endometrial
neoplasms. Methods: For this study, 28 patients with endometrial neoplasms as well as 22 controls were studied.
Analysis of the promoter methylation regions of PTEN and APC genes were performed by Methylation-Specific PCR.
Results: The frequency of PTEN and APC genes promoter methylation was 28.57% and 17.86% in tumor tissues, and
11.54% and 3.85% in blood samples, respectively. We found a significant relationship between blood and tissue in
PTEN methylation (p = 0.0353). Additionally, we determined a closely significant difference between normal tissue
and tumor tissue of the PTEN gene (p = 0.0787) and blood and tissue samples of the APC gene in methylated promoter
regions (p=0.0623). Furthermore, these results suggest that there is no significant relationship between the promoter
methylation of PTEN and APC with clinical characteristics. Conclusion: DNA methylation deficiency is a well known
highlighted factor in tumorigenesis, therefore the promoter hypermethylation of PTEN and APC can be indicated as a
risk factor in endometrial neoplasms.- انتشار مقاله: 07-04-1397
- نویسندگان: Tayebeh Ghazanfari,Golnaz Asaadi Tehrani,Parisa Maziri
- مشاهده