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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Metastasis,Invasion,Rhinacanthin-C,migration
- چکیده:
- چکیده انگلیسی: Cholangiocarcinoma is a malignant tumor with high metastatic and mortality rates. We investigated the effects
of rhinacanthin-C on cell proliferation, migration, invasion and the expression of proteins regulating cancer cell
invasion-regulated proteins in a cholangiocarcinoma (KKU-M156) cell line. Cytotoxicity of rhinacanthin-C was
determined by the SRB assay. Using wound-migration, chamber-migration and chamber-invasion assays, we assessed
the effects of rhinacanthin-C against KKU-M156 cells. The activities of matrix metalloproteinases 2 and 9 (MMP-2,
MMP-9) and urokinase-type plasminogen activator (uPA) were determined using gelatinase and uPA zymography
assays. The expression of invasion-regulated proteins was investigated using western-blot analysis. After treatment
with rhinacanthin-C, KKU-M156 cells exhibited antiproliferative effects in a dose-dependent manner with greater
efficacy than in Vero cells: IC50 values were 1.50 and 2.37 μM, respectively. Rhinacanthin-C significantly inhibited cell
migration and invasion of KKU-M156 cells in a dose-dependent manner. Consistent with this observation, treatment
with rhinacanthin-C was associated with a decrease in the expression levels of FAK, p-FAK, MMP-2, and a decrease in
the levels of p38-, JNK1/2- and ERK1/2-MAPK pathways as well as inhibiting NF-κB/p65 expression and translocation
of NF-κB/p65 to the nucleus. We have shown for the first time that the anti-metastatic effects of rhinacanthin-C on
KKU-M156 cells are mediated via inhibition of the expression of invasion-regulated proteins. Rhinacanthin-C may
deserve consideration as a potential agent for the treatment of cholangiocarcinoma.- انتشار مقاله: 15-03-1397
- نویسندگان: Parichart Boueroy,Sunitta Saensa‑Ard,Pongpun Siripong,Sakawrat Kanthawong,Chariya Hahnvajanawong
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Liver Fluke,Cyprinoids fish,Opisthorchis viverini,heating,Fermentation
- چکیده:
- چکیده انگلیسی:
Consumption of fluke-free fish is the most important factor in controlling Opisthorchis viverrini (OV) infection in endemic areas such as northeast Thailand and thereby reducing the risk of cholangiocarcinoma. Cooking fish is the best way to avoid infection; however, the cultural practice of eating raw or fermented fish is difficult to change. We investigated the food preparation process, using freezing, heating and fermentation to kill OV metacercariae in fish. Uncooked cyprinid fish infected with OV were divided into three groups: refrigerated at 4 oC for 24, 48 or 72 h (control group); frozen at -20 oC for 24, 48 or 72 h; or heated by microwaving (at 400 or 800 W) or boiling at 90 oC for 1, 5 or 10 min. Moreover, pickled (fermented) fish were divided into two groups: refrigerated at 4 oC (control) or frozen at -20 oC for 24 or 48 h. The infectivity of recovered metacercariae was confirmed by infecting hamsters with OV and then evaluating the recovery of adult worms after 1 month. We found that a heating process, by boiling or microwaving at 400 or 800 W for at least 5 min, could kill OV metacercariae, and freezing pickled fish at -20 oC for 48 h could kill OV metacercariae in all sizes of fish. The present study found that heating and freezing processes, as well as the fermentation process under optimal conditions, could kill OV metacercariae in a timely manner. This knowledge is valuable for implementation in endemic areas to control OV infection and cholangiocarcinoma.- انتشار مقاله: 09-11-1395
- نویسندگان: Panupan Sripan,Thidarut Boonmars,Jiraporn Songsri,Ratchadawan Aukkanimart,Pranee Sriraj,Panaratana Ratanasuwan,Parichart Boueroy,Apiporn Suwannatrai,Surasit Aunpromma,Narong Khuntikeo,Watcharin Loilome,Nisana Namwat,Puangrat Yongvanit,Aung Phyo Wai,Sukhonthip Khueangchaingkhwang,Wu Zhiliang,Benjamabhorn Pumhirunroj,Atchara Artchayasawat,Sirintip Boonjaraspinyo
- مشاهده